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Excretory-secretory product of newly excysted metacercariae of Paragonimus westermani directly induces eosinophil apoptosis

DC FieldValueLanguage
dc.contributor.author신명헌-
dc.date.accessioned2019-11-11T05:19:37Z-
dc.date.available2019-11-11T05:19:37Z-
dc.date.issued2000-
dc.identifier.issn0023-4001-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/171782-
dc.description.abstractEosinophils are important effector cells in host defense against parasites. Excretory-secretory product (ESP) produced by helminthic worms plays important roles in the uptake of nutrients, migration in the host tissue, and in immune modulation. However, little is known about the ability of the ESP to directly trigger eosinophil apoptosis. This study investigated whether the ESP of newly excysted metacercariae of Paragonimus westermani could induce apoptosis in human eosinophils. Apoptosis was assayed by staining the cells with FITC-annexin V, and the cells were analyzed by flow cytometry. It was found that the ESP of newly excysted metacercariae of P. westermani induced a direct time- and concentration-dependent increase in the rate of constitutive apoptosis in mature human eosinophils. Eosinophil apoptosis was first apparent 3 hr after treatment with the ESP and continued to increase after 6 hr of incubation with respect to the cells cultured in the absence of the ESP. While only 2.8% of the eosinophils incubated in the medium for 3 hr were apoptotic, 7.6%, 10.9% and 22.6% of the eosinophils treated with 10, 30 and 100 micrograms/ml ESP were apoptotic, respectively. This result suggests that the ESP of newly excysted metacercariae of P. westermani directly induce eosinophil apoptosis, which may be important for the survival of the parasites and the reduction of eosinophilic inflammation in vivo.-
dc.description.statementOfResponsibilityopen-
dc.languageEnglish, Korean-
dc.publisherKorean Society for Parasitology-
dc.relation.isPartOfKorean Journal of Parasitology-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.subject.MESHAnimals-
dc.subject.MESHApoptosis*-
dc.subject.MESHCells, Cultured-
dc.subject.MESHEosinophils/cytology-
dc.subject.MESHEosinophils/physiology*-
dc.subject.MESHHelminth Proteins/biosynthesis-
dc.subject.MESHHelminth Proteins/physiology*-
dc.subject.MESHHost-Parasite Interactions-
dc.subject.MESHHumans-
dc.subject.MESHLarva-
dc.subject.MESHParagonimus*/metabolism-
dc.titleExcretory-secretory product of newly excysted metacercariae of Paragonimus westermani directly induces eosinophil apoptosis-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Environmental Medical Biology (환경의생물학교실)-
dc.contributor.googleauthorMyeong Heon Shin-
dc.identifier.doi10.3347/kjp.2000.38.1.17-
dc.contributor.localIdA02099-
dc.relation.journalcodeJ01957-
dc.identifier.eissn1738-0006-
dc.identifier.pmid10743354-
dc.contributor.alternativeNameShin, Myeong Heon-
dc.contributor.affiliatedAuthor신명헌-
dc.citation.volume38-
dc.citation.number1-
dc.citation.startPage17-
dc.citation.endPage23-
dc.identifier.bibliographicCitationKorean Journal of Parasitology, Vol.38(1) : 17-23, 2000-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers

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