Cited 81 times in
Effects of gamma radiation on the conformational and antigenic properties of a heat-stable major allergen in brown shrimp.
DC Field | Value | Language |
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dc.contributor.author | 박중원 | - |
dc.contributor.author | 홍천수 | - |
dc.date.accessioned | 2019-11-11T05:12:44Z | - |
dc.date.available | 2019-11-11T05:12:44Z | - |
dc.date.issued | 2000 | - |
dc.identifier.issn | 0362-028X | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/171700 | - |
dc.description.abstract | This study was performed to evaluate the application of food irradiation technology as a method for reducing shrimp allergy without adverse effects. Shrimp heat-stable protein (HSP) was isolated and gamma irradiated at 0, 1, 3, 5, 7, or 10 kGy in the condition of solution (1 mg/ml), and fresh shrimp was also irradiated. Conformational change of irradiated HSP was monitored by means of spectrometric measures, enzyme-linked immunosorbent assay with mouse monoclonal antibody, or human patients' sera and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The ability of the immunoglobulin E of patients allergic to shrimp to bind to irradiated HSP was dose dependently reduced. The amount of intact HSP in an irradiated solution was reduced by gamma irradiation, depending on the dose. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that the main band disappeared and the traces induced from coagulation appeared at a higher molecular weight zone. The binding ability of immunoglobulin E to allergens in the extracts from irradiated shrimp decreased, depending on the dose. The results provide a new method so that food irradiation technology can be applied to reduce allergenicity of shrimp. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | International Association for Food Protection | - |
dc.relation.isPartOf | Journal of Food Protection | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Allergens/chemistry | - |
dc.subject.MESH | Allergens/immunology | - |
dc.subject.MESH | Allergens/isolation & purification | - |
dc.subject.MESH | Allergens/radiation effects* | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Decapoda (Crustacea)/immunology | - |
dc.subject.MESH | Decapoda (Crustacea)/radiation effects* | - |
dc.subject.MESH | Dose-Response Relationship, Radiation | - |
dc.subject.MESH | Electrophoresis, Polyacrylamide Gel | - |
dc.subject.MESH | Enzyme-Linked Immunosorbent Assay | - |
dc.subject.MESH | Food Irradiation* | - |
dc.subject.MESH | Gamma Rays* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Immunoglobulin E/immunology | - |
dc.subject.MESH | Molecular Weight | - |
dc.subject.MESH | Protein Conformation/radiation effects | - |
dc.subject.MESH | Spectrometry, Fluorescence | - |
dc.subject.MESH | Surface Properties/radiation effects | - |
dc.title | Effects of gamma radiation on the conformational and antigenic properties of a heat-stable major allergen in brown shrimp. | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학교실) | - |
dc.contributor.googleauthor | MYUNG-WOO BYUN | - |
dc.contributor.googleauthor | JAE-HUN KIM | - |
dc.contributor.googleauthor | JU-WOON LEE | - |
dc.contributor.googleauthor | JUNG-WON PARK | - |
dc.contributor.googleauthor | CHEIN-SOO HONG | - |
dc.contributor.googleauthor | IL-JUN KANG | - |
dc.identifier.doi | 10.4315/0362-028x-63.7.940 | - |
dc.contributor.localId | A01681 | - |
dc.contributor.localId | A04448-1 | - |
dc.relation.journalcode | J03685 | - |
dc.identifier.eissn | 1944-9097 | - |
dc.identifier.pmid | 10914665 | - |
dc.contributor.alternativeName | Park, Jung Won | - |
dc.contributor.affiliatedAuthor | 박중원 | - |
dc.contributor.affiliatedAuthor | 홍천수 | - |
dc.citation.volume | 63 | - |
dc.citation.number | 7 | - |
dc.citation.startPage | 940 | - |
dc.citation.endPage | 944 | - |
dc.identifier.bibliographicCitation | Journal of Food Protection, Vol.63(7) : 940-944, 2000 | - |
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