Cited 126 times in
CD44s expression in human colon carcinomas influences growth of liver metastases
DC Field | Value | Language |
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dc.contributor.author | 최승호 | - |
dc.date.accessioned | 2019-11-11T05:09:34Z | - |
dc.date.available | 2019-11-11T05:09:34Z | - |
dc.date.issued | 2000 | - |
dc.identifier.issn | 0020-7136 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/171658 | - |
dc.description.abstract | CD44 is a family of cell-surface adhesion molecules which exist in several isoforms arising from mRNA alternative. Malignant transformation of colonic mucosa is associated with alterations in CD44 expression, which result in up-regulation of high-molecular-weight CD44 isoforms and down-regulation of CD44s. We have demonstrated that stable transfection of CD44s into colon-carcinoma cell lines reduces their tumorigenicity. To understand the influence of CD44s expression on the metastatic potential of human colon carcinomas, we measured the ability of several different CD44s-transfected colon carcinomas to establish experimental liver metastases following splenic inoculation in mice. We observed that introduction of CD44s into 2 different human colon carcinoma cell lines, HT29 and KM12C6, resulted in reduced growth of liver metastases by as much as 75%. To explore the relationship between hyaluronate adhesion and metastasis, we transfected HT29 cells with cDNA encoding a mutant CD44s that does not bind to hyaluronate. HT29 transfectants expressing this mutant CD44s demonstrate an 84% reduction in growth of liver metastases, despite minimal binding to hyaluronate by the mutant CD44s. In concert, these results indicate that CD44s down-regulation, which occurs with malignant transformation of colonic mucosa, is associated with enhanced growth of experimental liver metastases. Consequently, the functional consequences of CD44s down-regulation in colon carcinomas may be just as significant as the consequences of up-regulation of other CD44 isoforms. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Wiley-Liss | - |
dc.relation.isPartOf | International Journal of Cancer | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Alternative Splicing | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Cell Division | - |
dc.subject.MESH | Colonic Neoplasms/genetics | - |
dc.subject.MESH | Colonic Neoplasms/pathology* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Hyaluronan Receptors/genetics | - |
dc.subject.MESH | Hyaluronan Receptors/physiology* | - |
dc.subject.MESH | Liver Neoplasms/pathology | - |
dc.subject.MESH | Liver Neoplasms/secondary* | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mice, Nude | - |
dc.subject.MESH | Protein Isoforms/genetics | - |
dc.subject.MESH | Protein Isoforms/physiology | - |
dc.subject.MESH | Recombinant Proteins/metabolism | - |
dc.subject.MESH | Transfection | - |
dc.subject.MESH | Tumor Cells, Cultured | - |
dc.title | CD44s expression in human colon carcinomas influences growth of liver metastases | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Surgery (외과학교실) | - |
dc.contributor.googleauthor | Seung Ho Choi | - |
dc.contributor.googleauthor | Kazuhisa Takahashi | - |
dc.contributor.googleauthor | Hiroshi Eto | - |
dc.contributor.googleauthor | Sam S. Yoon | - |
dc.contributor.googleauthor | Kenneth K. Tanabe | - |
dc.identifier.doi | 10.1212/WNL.54.1.65 | - |
dc.contributor.localId | A04102 | - |
dc.relation.journalcode | J01092 | - |
dc.identifier.eissn | 1097-0215 | - |
dc.identifier.pmid | 10699925 | - |
dc.contributor.alternativeName | Choi, Seung Ho | - |
dc.contributor.affiliatedAuthor | 최승호 | - |
dc.citation.volume | 85 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 523 | - |
dc.citation.endPage | 526 | - |
dc.identifier.bibliographicCitation | International Journal of Cancer, Vol.85(4) : 523-526, 2000 | - |
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