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Specific determination of endothelial cell viability in the whole cell fraction from cryopreserved canine femoral veins using flow-cytometry.

Authors
 Jong‐Chul Park  ;  Hak‐Joon Sung  ;  Dong Hee Lee  ;  Young Hwan Park  ;  Bum Koo Cho  ;  Hwal Suh 
Citation
 Artificial Organs, Vol.24(10) : 829-833, 2000 
Journal Title
 Artificial Organs 
ISSN
 0160-564X 
Issue Date
2000
MeSH
Animals ; Cell Survival ; Cryopreservation ; Dogs ; Endothelium, Vascular/cytology* ; Endothelium, Vascular/metabolism ; Female ; Femoral Vein ; Flow Cytometry* ; Humans ; Male ; Middle Aged ; Saphenous Vein ; Staining and Labeling
Keywords
Ceramic ; Dialysate ; Membranes ; Sterilization ; Water
Abstract
An efficient method for specifically determining the viability of endothelial cells (EC) from cells dissociated from the human saphenous vein was investigated. Three different methods, trypan blue staining assay, [3H]-proline incorporation assay, and flow cytometry (FCM), combined with the fluorescein isothiocyanate conjugated with Griffonia simplicifolia agglutins (GS1-FITC)/propidium iodide (PI) double staining, were used. Both trypan blue staining and [3H] proline incorporation assays demonstrated less sensitivity to determine viability of EC differentially from the other cells. FITC-GS1 showed prominent binding to the vascular EC and could be counted by FCM including PI on dead cells. Following the cryopreservation process, the GS1-FITC/PI FCM analytical method was adopted to test simultaneously the viability of whole cells and EC from the same tissue, human saphenous veins, and mongrel dogs' femoral veins after harvesting, antibiotic solution treatment, and thawing. The viability of the whole cells from veins decreased with a significant difference (p < 0.05) from that of EC after thawing.
Full Text
https://onlinelibrary.wiley.com/doi/abs/10.1046/j.1525-1594.2000.06514-2.x
DOI
10.1046/j.1525-1594.2000.06514-2.x
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Thoracic and Cardiovascular Surgery (흉부외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
Yonsei Authors
Park, Young Hwan(박영환) ORCID logo https://orcid.org/0000-0001-9802-8017
Park, Jong Chul(박종철) ORCID logo https://orcid.org/0000-0003-0083-5991
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/171642
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