Cited 31 times in
Evaluation of cardiac adrenergic neuronal damage in rats with doxorubicin-induced cardiomyopathy using iodine-131 MIBG autoradiography and PGP 9.5 immunohistochemistry
DC Field | Value | Language |
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dc.contributor.author | 양우익 | - |
dc.contributor.author | 이종두 | - |
dc.contributor.author | 하종원 | - |
dc.date.accessioned | 2019-11-11T05:07:13Z | - |
dc.date.available | 2019-11-11T05:07:13Z | - |
dc.date.issued | 2000 | - |
dc.identifier.issn | 1619-7070 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/171628 | - |
dc.description.abstract | Doxorubicin is one of the most useful anticancer agents, but its repeated administration can induce irreversible cardiomyopathy as a major complication. The purpose of this study was to investigate doxorubicin toxicity on cardiac sympathetic neurons using iodine-131-metaiodobenzylguanidine (MIBG) and protein gene product (PGP) 9.5 immunohistochemistry, which is a marker of cardiac innervation. Wistar rats were treated with doxorubicin (2 mg/kg, i.v.) once a week for 4 (n=5), 6 (n=6) or 8 (n=7) weeks consecutively. Left ventricular ejection fraction (LVEF), calculated by M-mode echocardiography, was used as an indicator of cardiac function. Plasma noradrenaline (NA) concentration was measured by high-performance liquid chromatography (HPLC). 131I-MIBG uptake of the left ventricular wall (24 ROIs) was measured by autoradiography. 131I-MIBG uptake pattern was compared with histopathological results, the neuronal population on PGP 9.5 immunohistochemistry and the degree of myocyte damage assessed using a visual scoring system on haematoxylin and eosin and Masson's trichrome staining. LVEF was significantly decreased in the 8-week group (P<0.05). The serum NA level also showed no statistical difference until 4 weeks and was significantly increased in the 8-week group (P<0.05). MIBG uptake was decreased in the 6- and 8-week groups (P<0.05), and was closely correlated with the reduction in the number of nerve fibres on PGP 9.5 stain. Myocyte damage was seen only in the 8-week group. Neuronal population and the 131I-MIBG uptake ratio of subepicardium to subendocardium were significantly increased (P<0.05) in the 8-week group as compared with the control group. It may be concluded that radioiodinated MIBG is a reliable marker for the detection of cardiac adrenergic neuronal damage in doxorubicin-induced cardiomyopathy; it detects such damage earlier than do other clinical parameters and in this study showed a good correlation with the reduction in the neuronal population on PGP 9.5 stain. The subendocardial layer appeared to be more vulnerable to doxorubicin than the subepicardium. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Springer-Verlag Berlin | - |
dc.relation.isPartOf | European Journal of Nuclear Medicine and Molecular Imaging | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | 3-Iodobenzylguanidine* | - |
dc.subject.MESH | Adrenergic Fibers/drug effects* | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Antibiotics, Antineoplastic/toxicity* | - |
dc.subject.MESH | Cardiomyopathies/chemically induced* | - |
dc.subject.MESH | Cardiomyopathies/diagnostic imaging | - |
dc.subject.MESH | Doxorubicin/toxicity* | - |
dc.subject.MESH | Heart/diagnostic imaging | - |
dc.subject.MESH | Heart/innervation* | - |
dc.subject.MESH | Immunohistochemistry | - |
dc.subject.MESH | Norepinephrine/blood | - |
dc.subject.MESH | Radionuclide Imaging | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Rats, Wistar | - |
dc.subject.MESH | Thiolester Hydrolases/analysis* | - |
dc.subject.MESH | Ubiquitin Thiolesterase | - |
dc.subject.MESH | Ventricular Function, Left/drug effects | - |
dc.title | Evaluation of cardiac adrenergic neuronal damage in rats with doxorubicin-induced cardiomyopathy using iodine-131 MIBG autoradiography and PGP 9.5 immunohistochemistry | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Pathology (병리학교실) | - |
dc.contributor.googleauthor | Tae Joo Jeon | - |
dc.contributor.googleauthor | Jong Doo Lee | - |
dc.contributor.googleauthor | Jong-Won Ha | - |
dc.contributor.googleauthor | Woo Ick Yang | - |
dc.contributor.googleauthor | Sang Ho Cho | - |
dc.identifier.doi | 10.1007/s002590050563 | - |
dc.contributor.localId | A02300 | - |
dc.contributor.localId | A03138 | - |
dc.contributor.localId | A04257 | - |
dc.relation.journalcode | J00833 | - |
dc.identifier.eissn | 1619-7089 | - |
dc.identifier.pmid | 10901455 | - |
dc.identifier.url | https://link.springer.com/article/10.1007%2Fs002590050563 | - |
dc.subject.keyword | Cardiomyopathy | - |
dc.subject.keyword | Doxorubicin | - |
dc.subject.keyword | Iodine-131 metaiodobenzylguanidine | - |
dc.subject.keyword | Immunohistochemistry | - |
dc.subject.keyword | Protein gene product 9.5 | - |
dc.contributor.alternativeName | Yang, Woo Ick | - |
dc.contributor.affiliatedAuthor | 양우익 | - |
dc.contributor.affiliatedAuthor | 이종두 | - |
dc.contributor.affiliatedAuthor | 하종원 | - |
dc.citation.volume | 27 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 686 | - |
dc.citation.endPage | 693 | - |
dc.identifier.bibliographicCitation | European Journal of Nuclear Medicine and Molecular Imaging, Vol.27(6) : 686-693, 2000 | - |
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