Cited 9 times in
Surface expression of HLA-DM on dendritic cells derived from CD34-positive bone marrow haematopoietic stem cells.
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 민유홍 | - |
dc.date.accessioned | 2019-11-11T05:03:31Z | - |
dc.date.available | 2019-11-11T05:03:31Z | - |
dc.date.issued | 2000 | - |
dc.identifier.issn | 0007-1048 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/171579 | - |
dc.description.abstract | HLA-DM has been known to be largely absent from the cell surface of antigen-presenting cells, accumulating instead in the intracellular compartment. In this study, we demonstrated that a population of HLA-DM-positive (HLA-DM+) dendritic cells (DCs) can be identified in an in vitro culture of CD34+ bone marrow haematopoietic stem cells. CD34+ bone marrow cells of healthy donors were used to generate DCs with the recombinant human cytokines granulocyte-macrophage colony-stimulating factor (GM-CSF), tumour necrosis factor alpha (TNF-alpha) and stem cell factor (SCF), both with and without interleukin 4 (IL-4). Flow cytometric analysis demonstrated that HLA-DM+ cells comprised 2.5 +/- 0.9% and 1.8 +/- 0.4% of the CD34+ cell-derived progeny in the presence of GM-CSF, TNF-alpha and SCF after 7 d and 14 d of culture respectively. The number of HLA-DM molecules expressed per HLA-DM+ cell on d 7 was significantly higher than that on d 14 (1410 +/- 47 versus 370 +/- 25, P < 0.05). The addition of IL-4 to the cytokines from the commencement of culture increased the proportion of HLA-DM+ cells and increased the number of HLA-DM molecules per HLA-DM+ cell significantly (P < 0.05). Although most of the HLA-DM+ cells expressed CD1a, CD80 or CD86 antigen, only a small proportion of CD1a+, CD80+ or CD86+ cells expressed HLA-DM. About half the HLA-DM+ cells expressed CD83. The addition of IL-4 resulted in a decrease in the expression of CD83 on the HLA-DM+ cells on d 7. Microscopic evaluations of sorted HLA-DM+ cells revealed the characteristic morphological features of DCs. Primary mixed lymphocyte cultures demonstrated that the HLA-DM+ cells elicited a vigorous proliferation of allogeneic T cells. The level of antigen-specific T-cell activation induced by antigen-pulsed, chloroquine-treated HLA-DM+ cells was substantially higher than that induced by HLA-DM- cells (P < 0.05). These results show that HLA-DM can be used as a useful DC lineage-specific marker, as well as a tool for the characterization of DCs and human immunotherapy. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Wiley-Blackwell | - |
dc.relation.isPartOf | British Journal of Haematology | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Antigens, CD1/immunology | - |
dc.subject.MESH | Antigens, CD34/immunology* | - |
dc.subject.MESH | B7-1 Antigen/immunology | - |
dc.subject.MESH | Cell Division | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Dendritic Cells/immunology* | - |
dc.subject.MESH | Flow Cytometry | - |
dc.subject.MESH | Granulocyte-Macrophage Colony-Stimulating Factor/immunology | - |
dc.subject.MESH | HLA-D Antigens/immunology* | - |
dc.subject.MESH | Hematopoietic Stem Cells/immunology* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Interleukin-4/immunology | - |
dc.subject.MESH | Lymphocytes/cytology | - |
dc.subject.MESH | Stem Cell Factor/immunology | - |
dc.subject.MESH | Tumor Necrosis Factor-alpha/immunology | - |
dc.title | Surface expression of HLA-DM on dendritic cells derived from CD34-positive bone marrow haematopoietic stem cells. | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학교실) | - |
dc.contributor.googleauthor | Yoo Hong Min | - |
dc.contributor.googleauthor | Seung Tae Lee | - |
dc.contributor.googleauthor | Kyung Mi Choi | - |
dc.contributor.googleauthor | Jee Sook Hahn | - |
dc.contributor.googleauthor | Yun Woong Ko | - |
dc.identifier.doi | 10.1046/j.1365-2141.2000.02180.x | - |
dc.contributor.localId | A01407 | - |
dc.relation.journalcode | J00409 | - |
dc.identifier.eissn | 1365-2141 | - |
dc.identifier.pmid | 10971396 | - |
dc.identifier.url | https://onlinelibrary.wiley.com/doi/abs/10.1046/j.1365-2141.2000.02180.x | - |
dc.subject.keyword | HLA‐DM | - |
dc.subject.keyword | CD34 | - |
dc.subject.keyword | bone marrow | - |
dc.subject.keyword | haematopoietic | - |
dc.subject.keyword | dendritic cell | - |
dc.contributor.alternativeName | Min, Yoo Hong | - |
dc.contributor.affiliatedAuthor | 민유홍 | - |
dc.citation.volume | 110 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 385 | - |
dc.citation.endPage | 393 | - |
dc.identifier.bibliographicCitation | British Journal of Haematology, Vol.110(2) : 385-393, 2000 | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.