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Identification and characterization of the major allergens of buckwheat

DC FieldValueLanguage
dc.contributor.author김규언-
dc.contributor.author박중원-
dc.contributor.author홍천수-
dc.contributor.author박중원-
dc.contributor.author홍천수-
dc.date.accessioned2019-11-11T04:59:43Z-
dc.date.available2019-11-11T04:59:43Z-
dc.date.issued2000-
dc.identifier.issn0105-4538-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/171528-
dc.description.abstractBACKGROUND: Buckwheat (BW) has been recognized as a common food allergen in Korea, Japan, and other countries. Until now, serologic findings of BW food-allergic patients and its major allergenic components have not been clarified. In this study, we analyzed the serologic findings of BW food allergy and characterized its major allergenic components. METHODS: Nineteen BW-allergic subjects with symptoms after BW ingestion and 15 asymptomatic control subjects with positive skin prick test to BW were recruited. BW-specific IgE was measured with the Pharmacia CAP kit. Allergenic components of BW were analyzed by IgE immunoblotting, periodate oxidation, two-dimensonal PAGE, and sequencing of N-terminal amino acids. RESULTS: From the BW-allergic patients and asymptomatic controls, the sensitivity (100%), specificity (53%), and negative (100%) and positive predictive values (73%) of Pharmacia CAP specific IgE for diagnosis were estimated. The prevalence of IgE binding to 24-kDa (pI 8.3), 16-kDa (pI 5.6), and 9-kDa (pI 5.0/ 6.0) allergens was higher than 50% in BW-allergic and asymptomatic subjects. However, the specific IgE to split 19-kDa (pI 6.5/7.0) allergens were more specifically found in BW-allergic patients than in asymptomatic subjects (78% vs 7%). N-terminal amino-acid sequences of 19-kDa and 16-kDa allergens showed moderate and weak homology to the 19-kDa globulin protein of rice and alpha-amylase/trypsin inhibitor of millet, respectively. The N-terminus of the 9-kDa isoallergens were not different from each other and were identified as the reported trypsin inhibitors of BW. Attenuation of the IgE binding to the 9-kDa allergen was found with periodate oxidation. CONCLUSIONS: The allergens of 24, 19, 16, and 9 kDa are strong candidates to be major allergens, and the 19-kDa allergen was relatively specific for BW-allergic patients. Moreover, measurement of BW-specific IgE and the features of immunoblotting should be very useful tools in the diagnosis of BW allergy.-
dc.description.statementOfResponsibilityrestriction-
dc.languageEnglish-
dc.publisherWiley-Blackwell-
dc.relation.isPartOfAllergy-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.subject.MESHAdolescent-
dc.subject.MESHAdult-
dc.subject.MESHAllergens/analysis*-
dc.subject.MESHAllergens/chemistry-
dc.subject.MESHAmino Acid Sequence-
dc.subject.MESHDouble-Blind Method-
dc.subject.MESHFemale-
dc.subject.MESHFood Hypersensitivity/etiology*-
dc.subject.MESHHumans-
dc.subject.MESHImmunoblotting-
dc.subject.MESHImmunoglobulin E/blood-
dc.subject.MESHMale-
dc.subject.MESHMolecular Sequence Data-
dc.subject.MESHMolecular Weight-
dc.subject.MESHPolygonaceae/immunology*-
dc.titleIdentification and characterization of the major allergens of buckwheat-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Pediatrics (소아청소년과학교실)-
dc.contributor.googleauthorJ. W. Park-
dc.contributor.googleauthorD. B. Kang-
dc.contributor.googleauthorC. W. Kim-
dc.contributor.googleauthorS. H. Ko-
dc.contributor.googleauthorH. Y. Yum*-
dc.contributor.googleauthorK. E. Kim*-
dc.contributor.googleauthorC.-S. Hong-
dc.contributor.googleauthorK. Y. Lee*-
dc.identifier.doi10.1034/j.1398-9995.2000.00763.x-
dc.contributor.localIdA00327-
dc.contributor.localIdA01681-
dc.contributor.localIdA01681-
dc.contributor.localIdA04448-1-
dc.contributor.localIdA04448-1-
dc.contributor.localIdA01681-
dc.contributor.localIdA01681-
dc.contributor.localIdA04448-1-
dc.contributor.localIdA04448-1-
dc.relation.journalcodeJ00062-
dc.identifier.eissn1398-9995-
dc.identifier.pmid11097313-
dc.identifier.urlhttps://onlinelibrary.wiley.com/doi/full/10.1034/j.1398-9995.2000.00763.x-
dc.subject.keywordbuckwheat allergy-
dc.subject.keywordmajor allergen-
dc.contributor.alternativeNameKim, Kyu Earn-
dc.contributor.affiliatedAuthor김규언-
dc.contributor.affiliatedAuthor박중원-
dc.contributor.affiliatedAuthor박중원-
dc.contributor.affiliatedAuthor홍천수-
dc.contributor.affiliatedAuthor홍천수-
dc.contributor.affiliatedAuthor박중원-
dc.contributor.affiliatedAuthor박중원-
dc.contributor.affiliatedAuthor홍천수-
dc.contributor.affiliatedAuthor홍천수-
dc.citation.volume55-
dc.citation.number11-
dc.citation.startPage1035-
dc.citation.endPage1041-
dc.identifier.bibliographicCitationAllergy, Vol.55(11) : 1035-1041, 2000-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pediatrics (소아청소년과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers

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