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Selecting short length nucleic acids localized in exosomes improves plasma EGFR mutation detection in NSCLC patients

DC FieldValueLanguage
dc.contributor.author신새암-
dc.contributor.author이경아-
dc.date.accessioned2019-10-28T02:05:37Z-
dc.date.available2019-10-28T02:05:37Z-
dc.date.issued2019-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/171479-
dc.description.abstractBackground: Exosomal nucleic acid (exoNA) is a feasible target to improve the sensitivity of EGFR mutation testing in non-small cell lung cancer patients with limited cell-free DNA (cfDNA) mutant copies. However, the type and size of target exoNA related to the sensitivity of EGFR mutation testing has not been explored extensively. Methods: The type and size of target exoNA related to the sensitivity of EGFR mutation testing was evaluated using ddPCR. A total of 47 plasma samples was tested using short-length exoTNA (exosomal DNA and RNA) and cfDNA. Results: The sensitivity of short-length exoTNA (76.5%) was higher than that of cfDNA (64.7%) for detecting EGFR mutations in NSCLC patients. In EGFR-mutant NSCLC patients with intrathoracic disease (M0/M1a) or cases with low-copy T790M, the positive rate was 63.6% (N = 7/11) and 45.5% (N = 5/11) for short-length exoTNA and cfDNA, respectively. On average, the number absolute mutant copies of short-length exoTNA were 1.5 times higher than that of cfDNA. The mutant allele copies (Ex19del and T790M) in short-length exoTNA were relatively well preserved at 4 weeks after storage. The difference (%) in absolute mutant allele copies (Ex19del) between 0 days and 4 weeks after storage was - 61.0% for cfDNA. Conclusion: Target nucleic acids and their size distribution may be critical considerations for selecting an extraction method and a detection assay. A short-length exoTNA (200 bp) contained more detectable tumor-derived nucleic acids than exoDNA (~ 200 bp length or a full-length) or cfDNA. Therefore, a short-length exoTNA as a sensitive biomarker might be useful to detect EGFR mutants for NSCLC patients with low copy number of the mutation target.-
dc.description.statementOfResponsibilityopen-
dc.languageEnglish-
dc.publisherBioMed Central-
dc.relation.isPartOfCancer Cell International-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.titleSelecting short length nucleic acids localized in exosomes improves plasma EGFR mutation detection in NSCLC patients-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Laboratory Medicine (진단검사의학교실)-
dc.contributor.googleauthorYoonjung Kim-
dc.contributor.googleauthorSaeam Shin-
dc.contributor.googleauthorBoyeon Kim-
dc.contributor.googleauthorKyung-A Lee-
dc.identifier.doi10.1186/s12935-019-0978-8-
dc.contributor.localIdA02108-
dc.contributor.localIdA02647-
dc.contributor.localIdA02647-
dc.relation.journalcodeJ00436-
dc.identifier.eissn1475-2867-
dc.identifier.pmid31582907-
dc.subject.keywordCirculating tumor DNA-
dc.subject.keywordEpidermal growth factor receptor-
dc.subject.keywordExtracellular vesicles-
dc.subject.keywordLiquid biopsy-
dc.subject.keywordNon-small cell lung cancer-
dc.subject.keywordddPCR-
dc.contributor.alternativeNameShin, Saeam-
dc.contributor.affiliatedAuthor신새암-
dc.contributor.affiliatedAuthor이경아-
dc.contributor.affiliatedAuthor이경아-
dc.citation.volume19-
dc.citation.startPage251-
dc.identifier.bibliographicCitationCancer Cell International, Vol.19 : 251, 2019-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers

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