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Effects of Oxytocin on Cell Proliferation in a Corticotroph Adenoma Cell Line

Authors
 Jung Soo Lim  ;  Young Woo Eom  ;  Eun Soo Lee  ;  Hyeong Ju Kwon  ;  Ja-Young Kwon  ;  Junjeong Choi  ;  Choon Hee Chung  ;  Young Suk Jo  ;  Eun Jig Lee 
Citation
 Endocrinology and Metabolism (대한내분비학회지), Vol.34(3) : 302-313, 2019 
Journal Title
 Endocrinology and Metabolism (대한내분비학회지) 
ISSN
 2093-596X 
Issue Date
2019
Keywords
ACTH-secreting pituitary adenoma ; Cell proliferation ; Corticotrophs ; Oxytocin
Abstract
BACKGROUND: Oxytocin (OXT) has been reported to act as a growth regulator in various tumor cells. However, there is a paucity of data on the influence of OXT on cell proliferation of corticotroph adenomas. This study aimed to examine whether OXT affects cell growth in pituitary tumor cell lines (AtT20 and GH3 cells) with a focus on corticotroph adenoma cells. METHODS: Reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay were conducted with AtT20 cells to confirm the effects of OXT on hormonal activity; flow cytometry was used to assess changes in the cell cycle after OXT treatment. Moreover, the impact of OXT on proliferating cell nuclear antigen (PCNA), nuclear factor κB, and mitogen-activated protein kinase signaling pathway was analyzed by Western blot. RESULTS: OXT treatment of 50 nM changed the gene expression of OXT receptor and pro-opiomelanocortin within a short time. In addition, OXT significantly reduced adrenocorticotropic hormone secretion within 1 hour. S and G2/M populations of AtT20 cells treated with OXT for 24 hours were significantly decreased compared to the control. Furthermore, OXT treatment decreased the protein levels of PCNA and phosphorylated extracellular-signal-regulated kinase (P-ERK) in AtT20 cells. CONCLUSION: Although the cytotoxic effect of OXT in AtT20 cells was not definite, OXT may blunt cell proliferation of corticotroph adenomas by altering the cell cycle or reducing PCNA and P-ERK levels. Further research is required to investigate the role of OXT as a potential therapeutic target in corticotroph adenomas.
Files in This Item:
T201903628.pdf Download
DOI
10.3803/EnM.2019.34.3.302
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Obstetrics and Gynecology (산부인과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Kwon, Ja Young(권자영) ORCID logo https://orcid.org/0000-0003-3009-6325
Lee, Eun Jig(이은직) ORCID logo https://orcid.org/0000-0002-9876-8370
Jo, Young Suk(조영석) ORCID logo https://orcid.org/0000-0001-9926-8389
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/171475
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