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Two Novel Bacteriophages Improve Survival in Galleria mellonella Infection and Mouse Acute Pneumonia Models Infected with Extensively Drug-Resistant Pseudomonas aeruginosa

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dc.contributor.author용동은-
dc.contributor.author전종수-
dc.date.accessioned2019-09-20T07:42:55Z-
dc.date.available2019-09-20T07:42:55Z-
dc.date.issued2019-
dc.identifier.issn0099-2240-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/171029-
dc.description.abstractExtensively drug-resistant Pseudomonas aeruginosa (XDR-PA) is a life-threatening pathogen that causes serious global problems. Here, we investigated two novel P. aeruginosa bacteriophages (phages), Bϕ-R656 and Bϕ-R1836, in vitro, in silico, and in vivo to evaluate the potential of phage therapy to control XDR-PA clinical strains. Bϕ-R656 and Bϕ-R1836 belong to the Siphoviridae family and exhibited broad host ranges which could lyse 18 (64%) and 14 (50%) of the 28 XDR-PA strains. In addition, the two phages showed strong bacteriolytic activity against XDR-PA host strains from pneumonia patients. The whole genomes of Bϕ-R656 and Bϕ-R1836 have linear double-stranded DNA of 60,919 and 37,714 bp, respectively. The complete sequence of Bϕ-R656 had very low similarity to the previously discovered P. aeruginosa phages in GenBank, but phage Bϕ-R1836 exhibited 98% and 91% nucleotide similarity to Pseudomonas phages YMC12/01/R24 and PA1/KOR/2010, respectively. In the two in vivo infection models, treatment with Bϕ-R656 and Bϕ-R1836 enhanced the survival of Galleria mellonella larvae (50% and 60%, respectively) at 72 h postinfection and pneumonia-model mice (66% and 83%, respectively) at 12 days postinfection compared with untreated controls. Treatment with Bϕ-R656 or Bϕ-R1836 also significantly decreased the bacterial load in the lungs of the mouse pneumonia model (>6 log10 CFU and >4 log10 CFU, respectively) on day 5.IMPORTANCE In this study, two novel P. aeruginosa phages, Bϕ-R656 and Bϕ-R1836, were evaluated in vitro, in silico, and in vivo for therapeutic efficacy and safety as an alternative antibacterial agent to control XDR-PA strains collected from pneumonia patients. Both phages exhibited potent bacteriolytic activity and greatly improved survival in G. mellonella larva infection and a mouse acute pneumonia model. Based on these results, we strongly predict that these two new phages could be used as fast-acting and safe alternative biological weapons against XDR-PA infections.-
dc.description.statementOfResponsibilityopen-
dc.formatapplication/pdf-
dc.languageEnglish-
dc.publisherAmerican Society for Microbiology-
dc.relation.isPartOfApplied and Environmental Microbiology-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.titleTwo Novel Bacteriophages Improve Survival in Galleria mellonella Infection and Mouse Acute Pneumonia Models Infected with Extensively Drug-Resistant Pseudomonas aeruginosa-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Laboratory Medicine (진단검사의학교실)-
dc.contributor.googleauthorJongsoo Jeon-
dc.contributor.googleauthorDongeun Yong-
dc.identifier.doi10.1128/AEM.02900-18-
dc.contributor.localIdA02423-
dc.relation.journalcodeJ00197-
dc.identifier.eissn1098-5336-
dc.identifier.pmid30824445-
dc.subject.keywordGalleria mellonella infection-
dc.subject.keywordPseudomonas aeruginosa-
dc.subject.keywordSiphoviridae-
dc.subject.keywordbacteriophage-
dc.subject.keywordbiofilm-
dc.subject.keywordmouse acute pneumonia-
dc.subject.keywordphage therapy-
dc.contributor.alternativeNameYong, Dong Eun-
dc.contributor.affiliatedAuthor용동은-
dc.citation.volume85-
dc.citation.number9-
dc.citation.startPagee02900-18-
dc.identifier.bibliographicCitationApplied and Environmental Microbiology, Vol.85(9) : e02900-18, 2019-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers

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