Cited 53 times in
Two Novel Bacteriophages Improve Survival in Galleria mellonella Infection and Mouse Acute Pneumonia Models Infected with Extensively Drug-Resistant Pseudomonas aeruginosa
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 용동은 | - |
dc.contributor.author | 전종수 | - |
dc.date.accessioned | 2019-09-20T07:42:55Z | - |
dc.date.available | 2019-09-20T07:42:55Z | - |
dc.date.issued | 2019 | - |
dc.identifier.issn | 0099-2240 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/171029 | - |
dc.description.abstract | Extensively drug-resistant Pseudomonas aeruginosa (XDR-PA) is a life-threatening pathogen that causes serious global problems. Here, we investigated two novel P. aeruginosa bacteriophages (phages), Bϕ-R656 and Bϕ-R1836, in vitro, in silico, and in vivo to evaluate the potential of phage therapy to control XDR-PA clinical strains. Bϕ-R656 and Bϕ-R1836 belong to the Siphoviridae family and exhibited broad host ranges which could lyse 18 (64%) and 14 (50%) of the 28 XDR-PA strains. In addition, the two phages showed strong bacteriolytic activity against XDR-PA host strains from pneumonia patients. The whole genomes of Bϕ-R656 and Bϕ-R1836 have linear double-stranded DNA of 60,919 and 37,714 bp, respectively. The complete sequence of Bϕ-R656 had very low similarity to the previously discovered P. aeruginosa phages in GenBank, but phage Bϕ-R1836 exhibited 98% and 91% nucleotide similarity to Pseudomonas phages YMC12/01/R24 and PA1/KOR/2010, respectively. In the two in vivo infection models, treatment with Bϕ-R656 and Bϕ-R1836 enhanced the survival of Galleria mellonella larvae (50% and 60%, respectively) at 72 h postinfection and pneumonia-model mice (66% and 83%, respectively) at 12 days postinfection compared with untreated controls. Treatment with Bϕ-R656 or Bϕ-R1836 also significantly decreased the bacterial load in the lungs of the mouse pneumonia model (>6 log10 CFU and >4 log10 CFU, respectively) on day 5.IMPORTANCE In this study, two novel P. aeruginosa phages, Bϕ-R656 and Bϕ-R1836, were evaluated in vitro, in silico, and in vivo for therapeutic efficacy and safety as an alternative antibacterial agent to control XDR-PA strains collected from pneumonia patients. Both phages exhibited potent bacteriolytic activity and greatly improved survival in G. mellonella larva infection and a mouse acute pneumonia model. Based on these results, we strongly predict that these two new phages could be used as fast-acting and safe alternative biological weapons against XDR-PA infections. | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/pdf | - |
dc.language | English | - |
dc.publisher | American Society for Microbiology | - |
dc.relation.isPartOf | Applied and Environmental Microbiology | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | Two Novel Bacteriophages Improve Survival in Galleria mellonella Infection and Mouse Acute Pneumonia Models Infected with Extensively Drug-Resistant Pseudomonas aeruginosa | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Laboratory Medicine (진단검사의학교실) | - |
dc.contributor.googleauthor | Jongsoo Jeon | - |
dc.contributor.googleauthor | Dongeun Yong | - |
dc.identifier.doi | 10.1128/AEM.02900-18 | - |
dc.contributor.localId | A02423 | - |
dc.relation.journalcode | J00197 | - |
dc.identifier.eissn | 1098-5336 | - |
dc.identifier.pmid | 30824445 | - |
dc.subject.keyword | Galleria mellonella infection | - |
dc.subject.keyword | Pseudomonas aeruginosa | - |
dc.subject.keyword | Siphoviridae | - |
dc.subject.keyword | bacteriophage | - |
dc.subject.keyword | biofilm | - |
dc.subject.keyword | mouse acute pneumonia | - |
dc.subject.keyword | phage therapy | - |
dc.contributor.alternativeName | Yong, Dong Eun | - |
dc.contributor.affiliatedAuthor | 용동은 | - |
dc.citation.volume | 85 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | e02900-18 | - |
dc.identifier.bibliographicCitation | Applied and Environmental Microbiology, Vol.85(9) : e02900-18, 2019 | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.