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A hepatocyte growth factor/MET-induced antiapoptotic pathway protects against radiation-induced salivary gland dysfunction

DC FieldValueLanguage
dc.contributor.author임재열-
dc.date.accessioned2019-07-23T06:58:05Z-
dc.date.available2019-07-23T06:58:05Z-
dc.date.issued2019-
dc.identifier.issn0167-8140-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/170396-
dc.description.abstractOBJECTIVE: Hepatocyte growth factor (HGF) and its receptor MET are expressed in the salivary glands during developmental stages and tumor formation; however, the function of HGF in injured salivary gland tissues remains unclear. The present study investigated the role of HGF in protecting the salivary glands against radiation-induced injury using an organotypic culture method. MATERIALS AND METHODS: Acinar-like organoids were formed by means of a three-dimensional (3D) human parotid tissue-derived spheroids (hPTS) culture method. Radioprotective effects of HGF on irradiated hPTS and signaling pathways on radioprotection were investigated. RESULTS: We detected MET expression in hPTS grown in a 3D culture. Treatment of irradiated hPTS with recombinant human HGF (rhHGF) restored salivary marker expression and secretory function of hPTS. Changes in the phosphorylation levels of apoptosis-related proteins through HGF-MET axis inhibited radiation-induced apoptosis. Treatment with PHA665752, a MET inhibitor, blocked MET-PI3K-AKT pathway, increased apoptosis, and suppressed the radioprotective effect of rhHGF against IR-induced damage of hPTS. CONCLUSIONS: These results suggest that HGF is a key effector of radioprotection and that HGF-MET-PI3K-AKT axis is involved in protecting the salivary glands from radiation-induced apoptosis.-
dc.description.statementOfResponsibilityopen-
dc.languageEnglish-
dc.publisherElsevier Scientific Publishers-
dc.relation.isPartOfRADIOTHERAPY AND ONCOLOGY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleA hepatocyte growth factor/MET-induced antiapoptotic pathway protects against radiation-induced salivary gland dysfunction-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Otorhinolaryngology (이비인후과학교실)-
dc.contributor.googleauthorYeo Jun Yoon-
dc.contributor.googleauthorHyun-Soo Shin-
dc.contributor.googleauthorJae-Yol Lim-
dc.identifier.doi10.1016/j.radonc.2019.05.012-
dc.contributor.localIdA03396-
dc.relation.journalcodeJ02597-
dc.identifier.eissn1879-0887-
dc.identifier.pmid31136962-
dc.subject.keywordHepatocyte growth factor-
dc.subject.keywordRadiation-
dc.subject.keyword Salivary gland-
dc.subject.keywordThree-dimensional cell culture-
dc.contributor.alternativeNameLim, Jae Yol-
dc.contributor.affiliatedAuthor임재열-
dc.citation.volume138-
dc.citation.startPage9-
dc.citation.endPage16-
dc.identifier.bibliographicCitationRADIOTHERAPY AND ONCOLOGY, Vol.138 : 9-16, 2019-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers

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