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CD133+/C-kit+Lin- endothelial progenitor cells in fetal circulation demonstrate impaired differentiation potency in severe preeclampsia

DC Field Value Language
dc.contributor.author권자영-
dc.contributor.author김영한-
dc.contributor.author김현옥-
dc.contributor.author박예진-
dc.contributor.author이준호-
dc.contributor.author정윤지-
dc.date.accessioned2019-07-23T06:52:32Z-
dc.date.available2019-07-23T06:52:32Z-
dc.date.issued2019-
dc.identifier.issn2210-7789-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/170351-
dc.description.abstractOBJECTIVES: Individuals delivered from preeclamptic pregnancies exhibit a long-term increased risk of developing cardiovascular and metabolic diseases, likely caused by aberrant fetal cell reprogramming incurred in utero. The present study investigated the functional impairment and epigenetic changes exhibited by endothelial progenitor cells derived from offspring born to preeclamptic pregnancies. STUDY DESIGN: The capacity of CD133+/C-kit+/Lin- (CKL-) human umbilical cord blood endothelial progenitor cells (EPCs) derived from gestationally matched normal and preeclamptic (n = 10 each) pregnancies to differentiate to form outgrowth endothelial cells (OECs) was assessed by observing both their morphology, and the number and size of generated OECs colonies. Likewise, OECs angiogenic function was evaluated via migration, adhesion, and tube-formation assays. EPCs from preeclampsia were cultured in normal-, and preeclampsia-derived serum-conditioned media to assess the effects of environmental factors on EPC differentiation potency and OEC angiogenic function, and finally, EPCs H3K4, H3K9, and H3K27 trimethylation levels were assayed. RESULTS: The preeclampsia-derived CKL- EPCs exhibited decreased H3K4 and H3K9 trimethylation levels, significantly delayed differentiation times, and a significant reduction in both their number of generated OECs colonies, and exhibited reduced OECs migration, adhesion, and tube formation activities compared to those achieved by the normal-derived EPCs. Interestingly, the reduced differentiation potency of the preeclampsia-derived EPCs was not rescued via exposure to normal serum. CONCLUSIONS: Exposure to preeclampsia significantly and irreversibly reduced CKL- EPC differentiation potency and OEC angiogenic function, likely reflecting incurred irreversible epigenetic changes.-
dc.description.statementOfResponsibilityrestriction-
dc.languageEnglish-
dc.publisherElsevier-
dc.relation.isPartOfPREGNANCY HYPERTENSION-AN INTERNATIONAL JOURNAL OF WOMENS CARDIOVASCULAR HEALTH-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAC133 Antigen/blood-
dc.subject.MESHAdult-
dc.subject.MESHAnalysis of Variance-
dc.subject.MESHCase-Control Studies-
dc.subject.MESHCell Movement-
dc.subject.MESHEndothelial Progenitor Cells/cytology-
dc.subject.MESHEpigenesis, Genetic-
dc.subject.MESHFemale-
dc.subject.MESHFetal Blood-
dc.subject.MESHHumans-
dc.subject.MESHPre-Eclampsia/blood-
dc.subject.MESHPre-Eclampsia/physiopathology-
dc.subject.MESHPregnancy-
dc.titleCD133+/C-kit+Lin- endothelial progenitor cells in fetal circulation demonstrate impaired differentiation potency in severe preeclampsia-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Obstetrics and Gynecology (산부인과학교실)-
dc.contributor.googleauthorYejin Park-
dc.contributor.googleauthorHwa Ji Lee-
dc.contributor.googleauthorYun Ji Jung-
dc.contributor.googleauthorHa Yan Kwon-
dc.contributor.googleauthorHeeyon Kim-
dc.contributor.googleauthorJoonHo Lee-
dc.contributor.googleauthorYoung-Han Kim-
dc.contributor.googleauthorHyun Ok Kim-
dc.contributor.googleauthorYong-Sun Maeng-
dc.contributor.googleauthorJa-Young Kwon-
dc.identifier.doi10.1016/j.preghy.2018.12.005-
dc.contributor.localIdA00246-
dc.contributor.localIdA00730-
dc.contributor.localIdA01122-
dc.contributor.localIdA04836-
dc.contributor.localIdA04846-
dc.contributor.localIdA04797-
dc.relation.journalcodeJ03639-
dc.identifier.eissn2210-7797-
dc.identifier.pmid30825912-
dc.identifier.urlhttps://www.sciencedirect.com/science/article/pii/S2210778918301284-
dc.subject.keywordCord blood-
dc.subject.keywordEndothelial Progenitor Cells-
dc.subject.keywordEpigenetic changes-
dc.subject.keywordOutgrowth Endothelial Cells-
dc.subject.keywordPreeclampsia-
dc.contributor.alternativeNameKwon, Ja Young-
dc.contributor.affiliatedAuthor권자영-
dc.contributor.affiliatedAuthor김영한-
dc.contributor.affiliatedAuthor김현옥-
dc.contributor.affiliatedAuthor박예진-
dc.contributor.affiliatedAuthor이준호-
dc.contributor.affiliatedAuthor정윤지-
dc.citation.volume15-
dc.citation.startPage146-
dc.citation.endPage153-
dc.identifier.bibliographicCitationPREGNANCY HYPERTENSION-AN INTERNATIONAL JOURNAL OF WOMENS CARDIOVASCULAR HEALTH, Vol.15 : 146-153, 2019-
dc.identifier.rimsid62836-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Obstetrics and Gynecology (산부인과학교실) > 1. Journal Papers

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