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MiR-150-5p May Contribute to Pathogenesis of Human Leiomyoma via Regulation of the Akt/p27Kip1 Pathway In Vitro

DC FieldValueLanguage
dc.contributor.author박주현-
dc.contributor.author서석교-
dc.contributor.author원영빈-
dc.contributor.author윤보현-
dc.contributor.author이병석-
dc.contributor.author이인하-
dc.contributor.author이재훈-
dc.contributor.author조시현-
dc.contributor.author최영식-
dc.date.accessioned2019-07-11T03:40:02Z-
dc.date.available2019-07-11T03:40:02Z-
dc.date.issued2019-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/170080-
dc.description.abstractUterine leiomyoma is found in ~50-80% of women of a reproductive age and is the most common reason for hysterectomy. Recently, posttranscriptional gene silencing by microRNAs (miRs) has been reported as a mechanism for regulating gene expression stability in the pathogenesis of uterine leiomyomas. In this study, miR microarray analysis of leiomyomas and paired myometrial tissue revealed numerous aberrantly expressed miRs, including miR-150. In functional assays, transfection with miR-150 mimic resulted in decreased migration and fibrosis, implying an inhibition of leiomyoma growth. To identify the target genes of miR-150 in leiomyoma, gene set analysis and network analysis were performed. To overcome the limitations of in silico analysis, changes in expression levels of hallmark genes in leiomyoma after transfection with a miR-150 mimic were also evaluated using qRT-PCR. As a result, the Akt/p27Kip1 pathway was presumed to be one of the target pathways of miR-150. After transfecting cultured leiomyoma cells with the miR-150 mimic, expression levels of its target gene Akt decreased, whereas those of p27Kip1 increased significantly. Our results suggest that miR-150 affects the cell cycle regulation in uterine leiomyoma through the Akt/p27Kip1 pathway.-
dc.description.statementOfResponsibilityopen-
dc.languageEnglish-
dc.publisherMDPI-
dc.relation.isPartOfINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleMiR-150-5p May Contribute to Pathogenesis of Human Leiomyoma via Regulation of the Akt/p27Kip1 Pathway In Vitro-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Obstetrics and Gynecology (산부인과학교실)-
dc.contributor.googleauthorJae Hoon Lee-
dc.contributor.googleauthorYoung Sik Choi-
dc.contributor.googleauthorJi Hyun Park-
dc.contributor.googleauthorHeeyon Kim-
dc.contributor.googleauthorInha Lee-
dc.contributor.googleauthorYoung Bin Won-
dc.contributor.googleauthorBo Hyon Yun-
dc.contributor.googleauthorJoo Hyun Park-
dc.contributor.googleauthorSeok Kyo Seo-
dc.contributor.googleauthorByung Seok Lee-
dc.contributor.googleauthorSiHyun Cho-
dc.identifier.doi10.3390/ijms20112684-
dc.contributor.localIdA01668-
dc.contributor.localIdA01888-
dc.contributor.localIdA01888-
dc.contributor.localIdA05484-
dc.contributor.localIdA05484-
dc.contributor.localIdA02555-
dc.contributor.localIdA02555-
dc.contributor.localIdA02795-
dc.contributor.localIdA02795-
dc.contributor.localIdA05497-
dc.contributor.localIdA05497-
dc.contributor.localIdA04636-
dc.contributor.localIdA04636-
dc.contributor.localIdA03846-
dc.contributor.localIdA03846-
dc.contributor.localIdA04114-
dc.contributor.localIdA04114-
dc.relation.journalcodeJ01133-
dc.identifier.eissn1422-0067-
dc.identifier.pmid31159158-
dc.subject.keywordAkt-
dc.subject.keywordleiomyoma-
dc.subject.keywordmicroRNA 150-5p-
dc.subject.keywordp27Kip1-
dc.contributor.alternativeNamePark, Joo Hyun-
dc.contributor.affiliatedAuthor박주현-
dc.contributor.affiliatedAuthor서석교-
dc.contributor.affiliatedAuthor서석교-
dc.contributor.affiliatedAuthor원영빈-
dc.contributor.affiliatedAuthor원영빈-
dc.contributor.affiliatedAuthor윤보현-
dc.contributor.affiliatedAuthor윤보현-
dc.contributor.affiliatedAuthor이병석-
dc.contributor.affiliatedAuthor이병석-
dc.contributor.affiliatedAuthor이인하-
dc.contributor.affiliatedAuthor이인하-
dc.contributor.affiliatedAuthor이재훈-
dc.contributor.affiliatedAuthor이재훈-
dc.contributor.affiliatedAuthor조시현-
dc.contributor.affiliatedAuthor조시현-
dc.contributor.affiliatedAuthor최영식-
dc.contributor.affiliatedAuthor최영식-
dc.citation.volume20-
dc.citation.number11-
dc.citation.startPageE2684-
dc.identifier.bibliographicCitationINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, Vol.20(11) : E2684, 2019-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Obstetrics and Gynecology (산부인과학교실) > 1. Journal Papers

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