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CRISPR/Cas9-mediated knockout of CD47 causes hemolytic anemia with splenomegaly in C57BL/6 mice
DC Field | Value | Language |
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dc.contributor.author | 남기택 | - |
dc.date.accessioned | 2019-05-29T05:18:51Z | - |
dc.date.available | 2019-05-29T05:18:51Z | - |
dc.date.issued | 2018 | - |
dc.identifier.issn | 1738-6055 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/169524 | - |
dc.description.abstract | CD47 (integrin-associated protein), a multi-spanning transmembrane protein expressed in all cells including red blood cells (RBCs) and leukocytes, interacts with signal regulatory protein α (SIRPα) on macrophages and thereby inhibits phagocytosis of RBCs. Recently, we generated a novel C57BL/6J CD47 knockout (CD47 -/- hereafter) mouse line by employing a CRISPR/Cas9 system at Center for Mouse Models of Human Disease, and here report their hematological phenotypes. On monitoring their birth and development, CD47 -/- mice were born viable with a natural male-to-female sex ratio and normally developed from birth through puberty to adulthood without noticeable changes in growth, food/water intake compared to their age and sex-matched wild-type littermates up to 26 weeks. Hematological analysis revealed a mild but significant reduction of RBC counts and hemoglobin in 16 week-old male CD47 -/- mice which were aggravated at the age of 26 weeks with increased reticulocyte counts and mean corpuscular volume (MCV), suggesting hemolytic anemia. Interestingly, anemia in female CD47 -/- mice became evident at 26 weeks, but splenomegaly was identified in both genders of CD47 -/- mice from the age of 16 weeks, consistent with development of hemolytic anemia. Additionally, helper and cytotoxic T cell populations were considerably reduced in the spleen, but not in thymus, of CD47 -/- mice, suggesting a crucial role of CD47 in proliferation of T cells. Collectively, these findings indicate that our CD47 -/- mice have progressive hemolytic anemia and splenic depletion of mature T cell populations and therefore may be useful as an in vivo model to study the function of CD47. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | Korean | - |
dc.publisher | 한국실험동물학회 | - |
dc.relation.isPartOf | Laboratory Animal Research | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | CRISPR/Cas9-mediated knockout of CD47 causes hemolytic anemia with splenomegaly in C57BL/6 mice | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | BioMedical Science Institute (의생명과학부) | - |
dc.contributor.googleauthor | Joo-Il Kim | - |
dc.contributor.googleauthor | Jin-Sung Park | - |
dc.contributor.googleauthor | Jina Kwak | - |
dc.contributor.googleauthor | Hyun-Jin Lim | - |
dc.contributor.googleauthor | Soo-Kyung Ryu | - |
dc.contributor.googleauthor | Euna Kwon | - |
dc.contributor.googleauthor | Kang-Min Han | - |
dc.contributor.googleauthor | Ki-Taek Nam | - |
dc.contributor.googleauthor | Han-Woong Lee | - |
dc.contributor.googleauthor | Byeong-Cheol Kang | - |
dc.identifier.doi | 10.5625/lar.2018.34.4.302 | - |
dc.contributor.localId | A01243 | - |
dc.relation.journalcode | J02149 | - |
dc.identifier.eissn | 2233-7660 | - |
dc.identifier.pmid | 30671119 | - |
dc.subject.keyword | CD47 | - |
dc.subject.keyword | CRISPR/Cas9 | - |
dc.subject.keyword | hemolytic anemia | - |
dc.subject.keyword | splenomegaly | - |
dc.contributor.alternativeName | Nam, Ki Taek | - |
dc.contributor.affiliatedAuthor | 남기택 | - |
dc.citation.volume | 34 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 302 | - |
dc.citation.endPage | 310 | - |
dc.identifier.bibliographicCitation | Laboratory Animal Research, Vol.34(4) : 302-310, 2018 | - |
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