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Browsing "1. Journal Papers" by Identifier : Objective: Entamoeba histolytica induces secretion of interleukin-8 by cultured colon epithelial cells. However, expression of cytokine genes in colon epithelial cells exposed to E. histolytica in the presence of enteric bacteria are not reported at all. We assessed the array of cytokine genes expressed by colon epithelial cells in response to co-culture with E. histolytica trophozoites and tested the hypothesis that enteric bacteria may alter E. histolytica induced expression of cytokine genes. Methods: After HT-29 human colon carcinoma epithelial cells were co-cultured with E. histolytica trophozoites in the presence or absence of E. coli, RNA was extracted from the epithelial cells by a guanidinium thiocyanate-phenol-chloroform method. Cytokine gene expression was assessed by quantitative RT-PCR. Results: IL-8 mRNA was upregulated following exposure to E. histolytica and this was paralleled by increased IL-8 protein secretion. GM-CSF and IL-1a/βmRNAs were also upregulated in these cells. When HT-29 cells were co-cultured with E. coli DH5a and E. histolytica, there was a synergistic increase in IL-8 gene as well as IL-1a and GM-CSF expression. Conclusion: These results suggest that enteric bacteria significantly affect the early proinflammatory signals produced in host tissues in response to E. histolytica infection.

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