Cited 28 times in
The bone anabolic effects of irisin are through preferential stimulation of aerobic glycolysis
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 임승길 | - |
dc.date.accessioned | 2019-04-03T07:47:59Z | - |
dc.date.available | 2019-04-03T07:47:59Z | - |
dc.date.issued | 2018 | - |
dc.identifier.issn | 8756-3282 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/167750 | - |
dc.description.abstract | Irisin, a recently identified hormone secreted by skeletal muscle in response to exercise, exhibits anabolic effects on the skeleton primarily through the stimulation of bone formation. However, the mechanism underlying the irisin-stimulated anabolic response remains largely unknown. To uncover the underlying mechanism, we biosynthesized recombinant irisin (r-irisin) using an Escherichia coli expression system and used it to treat several osteoblast cell types. Our synthesized r-irisin could promote proliferation and differentiation of osteoblasts as evidenced by enhanced expression of osteoblast-specific transcriptional factors, including Runt-related transcription factor-2 (Runx2), Oster (Osx), as well as early osteoblastic differentiation markers such as alkaline phosphatase (Alp) and collagen type I alpha 1 (Col1a1). Furthermore, we showed that the promotion of r-irisin on the proliferation and differentiation of osteoblast lineage cells are preferentially through aerobic glycolysis, as indicated by the enhanced abundance of representative enzymes such as lactate dehydrogenase A (LDHA) and pyruvate dehydrogenase kinase 1 (PDK1), together with increased lactate levels. Suppression of r-irisin-mediated aerobic glycolysis with Dichloroacetate blunted its anabolic effects. The favorite of the aerobic glycolysis after r-irisin treatment was then confirmed in primary calvarial cells by metabolic analysis using gas chromatography-mass spectrometry. Thus, our results suggest that the anabolic actions of r-irisin on the regulation of osteoblast lineage cells are preferentially through aerobic glycolysis, which may help to develop new irisin-based bone anabolic agents. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Elsevier Science | - |
dc.relation.isPartOf | BONE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | The bone anabolic effects of irisin are through preferential stimulation of aerobic glycolysis | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학교실) | - |
dc.contributor.googleauthor | Dongdong Zhang | - |
dc.contributor.googleauthor | ChuHyun Bae | - |
dc.contributor.googleauthor | Junghak Lee | - |
dc.contributor.googleauthor | Jiho Lee | - |
dc.contributor.googleauthor | Zeyu Jin | - |
dc.contributor.googleauthor | Myeongmo Kang | - |
dc.contributor.googleauthor | Young Suk Cho | - |
dc.contributor.googleauthor | Jeong-Han Kim | - |
dc.contributor.googleauthor | Weontae Lee | - |
dc.contributor.googleauthor | Sung-Kil Lim | - |
dc.identifier.doi | 10.1016/j.bone.2018.05.013 | - |
dc.contributor.localId | A03375 | - |
dc.relation.journalcode | J00381 | - |
dc.identifier.eissn | 1873-2763 | - |
dc.identifier.pmid | 29775761 | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S8756328218302011 | - |
dc.subject.keyword | Aerobic glycolysis | - |
dc.subject.keyword | Anabolism | - |
dc.subject.keyword | Irisin | - |
dc.subject.keyword | Osteoblast | - |
dc.subject.keyword | TCA cycle | - |
dc.contributor.alternativeName | Lim, Sung Kil | - |
dc.contributor.affiliatedAuthor | 임승길 | - |
dc.citation.volume | 114 | - |
dc.citation.startPage | 150 | - |
dc.citation.endPage | 160 | - |
dc.identifier.bibliographicCitation | BONE, Vol.114 : 150-160, 2018 | - |
dc.identifier.rimsid | 58347 | - |
dc.type.rims | ART | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.