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p38α MAPK is required for tooth morphogenesis and enamel secretion

DC FieldValueLanguage
dc.contributor.author박광환-
dc.date.accessioned2019-01-17T16:40:28Z-
dc.date.available2019-01-17T16:40:28Z-
dc.date.issued2015-
dc.identifier.issn0021-9258-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/166886-
dc.description.abstractAn improved understanding of the molecular pathways that drive tooth morphogenesis and enamel secretion is needed to generate teeth from organ cultures for therapeutic implantation or to determine the pathogenesis of primary disorders of dentition (Abdollah, S., Macias-Silva, M., Tsukazaki, T., Hayashi, H., Attisano, L., and Wrana, J. L. (1997) J. Biol. Chem. 272, 27678-27685). Here we present a novel ectodermal dysplasia phenotype associated with conditional deletion of p38α MAPK in ectodermal appendages using K14-cre mice (p38α(K14) mice). These mice display impaired patterning of dental cusps and a profound defect in the production and biomechanical strength of dental enamel because of defects in ameloblast differentiation and activity. In the absence of p38α, expression of amelogenin and β4-integrin in ameloblasts and p21 in the enamel knot was significantly reduced. Mice lacking the MAP2K MKK6, but not mice lacking MAP2K MKK3, also show the enamel defects, implying that MKK6 functions as an upstream kinase of p38α in ectodermal appendages. Lastly, stimulation with BMP2/7 in both explant culture and an ameloblast cell line confirm that p38α functions downstream of BMPs in this context. Thus, BMP-induced activation of the p38α MAPK pathway is critical for the morphogenesis of tooth cusps and the secretion of dental enamel.-
dc.description.statementOfResponsibilityopen-
dc.languageEnglish-
dc.publisherAmerican Society for Biochemistry and Molecular Biology-
dc.relation.isPartOfJournal of Biological Chemistry-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAmeloblasts/cytology-
dc.subject.MESHAmeloblasts/metabolism*-
dc.subject.MESHAmelogenin/genetics-
dc.subject.MESHAmelogenin/metabolism-
dc.subject.MESHAnimals-
dc.subject.MESHBone Morphogenetic Protein 2/genetics-
dc.subject.MESHBone Morphogenetic Protein 2/metabolism-
dc.subject.MESHBone Morphogenetic Protein 7/genetics-
dc.subject.MESHBone Morphogenetic Protein 7/metabolism-
dc.subject.MESHCell Differentiation-
dc.subject.MESHCell Proliferation-
dc.subject.MESHDental Enamel/cytology-
dc.subject.MESHDental Enamel/growth & development-
dc.subject.MESHDental Enamel/metabolism*-
dc.subject.MESHGene Expression Regulation, Developmental*-
dc.subject.MESHIncisor/cytology-
dc.subject.MESHIncisor/growth & development-
dc.subject.MESHIncisor/metabolism*-
dc.subject.MESHIntegrin beta4/genetics-
dc.subject.MESHIntegrin beta4/metabolism-
dc.subject.MESHMAP Kinase Kinase 3/genetics-
dc.subject.MESHMAP Kinase Kinase 3/metabolism-
dc.subject.MESHMAP Kinase Kinase 6/genetics-
dc.subject.MESHMAP Kinase Kinase 6/metabolism-
dc.subject.MESHMice-
dc.subject.MESHMice, Transgenic-
dc.subject.MESHMitogen-Activated Protein Kinase 14/genetics-
dc.subject.MESHMitogen-Activated Protein Kinase 14/metabolism*-
dc.subject.MESHOdontogenesis/genetics*-
dc.subject.MESHSignal Transduction-
dc.subject.MESHTissue Culture Techniques-
dc.subject.MESHp21-Activated Kinases/genetics-
dc.subject.MESHp21-Activated Kinases/metabolism-
dc.titlep38α MAPK is required for tooth morphogenesis and enamel secretion-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Orthopedic Surgery (정형외과학교실)-
dc.contributor.googleauthorMatthew B. Greenblatt-
dc.contributor.googleauthorJung-Min Kim-
dc.contributor.googleauthorHwanhee Oh-
dc.contributor.googleauthorKwang Hwan Park-
dc.contributor.googleauthorMin-Kyung Choo-
dc.contributor.googleauthorYasuyo Sano-
dc.contributor.googleauthorCoralee E. Tye-
dc.contributor.googleauthorZiedonis Skobe-
dc.contributor.googleauthorRoger J. Davis-
dc.contributor.googleauthorJin Mo Park-
dc.contributor.googleauthorMarianna Bei-
dc.contributor.googleauthorLaurie H. Glimcher-
dc.contributor.googleauthorJae-Hyuck Shim-
dc.identifier.doi10.1074/jbc.M114.599274-
dc.contributor.localIdA01437-
dc.relation.journalcodeJ01258-
dc.identifier.eissn1083-351X-
dc.identifier.pmid25406311-
dc.subject.keywordAmeloblast-
dc.subject.keywordBone Morphogenetic Protein (BMP)-
dc.subject.keywordEctodermal Dysplasia-
dc.subject.keywordMicrotubule-associated Protein (MAP)-
dc.subject.keywordSMAD Transcription Factor-
dc.subject.keywordTooth Development-
dc.subject.keywordp38 MAPK-
dc.contributor.alternativeNamePark, Kwang Hwan-
dc.contributor.affiliatedAuthor박광환-
dc.citation.volume290-
dc.citation.number1-
dc.citation.startPage284-
dc.citation.endPage295-
dc.identifier.bibliographicCitationJournal of Biological Chemistry, Vol.290(1) : 284-295, 2015-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers

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