Cited 11 times in
Expression of human miR-200b-3p and -200c-3p in cytomegalovirus-infected tissues
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이경화 | - |
dc.contributor.author | 임범진 | - |
dc.contributor.author | 한상훈 | - |
dc.date.accessioned | 2019-01-15T17:11:52Z | - |
dc.date.available | 2019-01-15T17:11:52Z | - |
dc.date.issued | 2018 | - |
dc.identifier.issn | 0144-8463 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/166855 | - |
dc.description.abstract | Human cytomegalovirus (HCMV) infection can cause inflammatory tissue-invasive end-organ diseases upon lytic replication. In humans, mature miR-200b-3p and -200c-3p suppress the synthesis of HCMV immediate early 2 (IE2) protein by binding to the 3'-UTR of the mRNA encoded by the unique long (UL) 122-123 region in human foreskin fibroblasts and pre-transplant peripheral blood mononuclear cells stimulated with HCMV. The present study aimed to quantitate the expression of Homo sapiens (hsa)-miR-200b-3p and 200c-3p in HCMV-infected tissues. We collected 240 HCMV-infected and 154 HCMV-non-infected, formalin-fixed, paraffin-embedded tissue samples of the gastrointestinal (GI) tract and bronchi/lungs. MiRNAs, HCMV, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were quantitated by quantitative reverse transcription-PCR (qRT-PCR) and quantitative PCR (qPCR) on the basis of standard curves generated using miRNA mimics, the HCMV strain from National Institute for Biological Standards and Control (NIBSC) 09/162, and GAPDH control. To avoid the effect of cell counts on the qRT-PCR and qPCR results, the data were normalized to GAPDH levels. HCMV-infected tissues had significantly lower levels of 200b-3p/GAPDH (3.03 ± 1.50 compared with 3.98 ± 1.08 log10 copies/μl, P<0.001) and 200c-3p/GAPDH (4.67 ± 1.84 compared with 6.35 ± 1.47 log10 copies/μl, P<0.001) than normal tissues. The values for 200b-3p/GAPDH (r = -0.51, P<0.001) and 200c-3p/GAPDH (r = -0.54, P<0.001) were significantly inversely correlated with HCMV load. Low tissue levels of 200b-3p and 200c-3p in humans are associated with cytopathic inflammation due to HCMV infection. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | England | - |
dc.publisher | 1573-4935 | - |
dc.relation.isPartOf | BIOSCIENCE REPORTS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Expression of human miR-200b-3p and -200c-3p in cytomegalovirus-infected tissues | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학교실) | - |
dc.contributor.googleauthor | Kyoung Hwa Lee | - |
dc.contributor.googleauthor | Beom Jin Lim | - |
dc.contributor.googleauthor | Victor H. Ferreira | - |
dc.contributor.googleauthor | Seo Yeon Min | - |
dc.contributor.googleauthor | Yeon-Mi Hong | - |
dc.contributor.googleauthor | Jeong-Hyeon Jo | - |
dc.contributor.googleauthor | Sang Hoon Han | - |
dc.identifier.doi | 10.1042/BSR20180961 | - |
dc.contributor.localId | A04620 | - |
dc.contributor.localId | A03363 | - |
dc.contributor.localId | A04286 | - |
dc.relation.journalcode | J03560 | - |
dc.identifier.eissn | 1573-4935 | - |
dc.identifier.pmid | 30366960 | - |
dc.identifier.url | http://www.bioscirep.org/content/38/6/BSR20180961.long | - |
dc.subject.keyword | cytomegalovirus | - |
dc.subject.keyword | immediate early protein 2 | - |
dc.subject.keyword | microRNA | - |
dc.subject.keyword | tissues | - |
dc.contributor.alternativeName | Lee, Kyoung Hwa | - |
dc.contributor.affiliatedAuthor | 이경화 | - |
dc.contributor.affiliatedAuthor | 임범진 | - |
dc.contributor.affiliatedAuthor | 한상훈 | - |
dc.citation.volume | 38 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | BSR20180961 | - |
dc.identifier.bibliographicCitation | BIOSCIENCE REPORTS, Vol.38(6) : BSR20180961, 2018 | - |
dc.identifier.rimsid | 58226 | - |
dc.type.rims | ART | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.