0 47

Cited 0 times in

Expression of human miR-200b-3p and -200c-3p in cytomegalovirus-infected tissues

DC FieldValueLanguage
dc.contributor.author이경화-
dc.contributor.author임범진-
dc.contributor.author한상훈-
dc.date.accessioned2019-01-15T17:11:52Z-
dc.date.available2019-01-15T17:11:52Z-
dc.date.issued2018-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/166855-
dc.description.abstractHuman cytomegalovirus (HCMV) infection can cause inflammatory tissue-invasive end-organ diseases upon lytic replication. In humans, mature miR-200b-3p and -200c-3p suppress the synthesis of HCMV immediate early 2 (IE2) protein by binding to the 3'-UTR of the mRNA encoded by the unique long (UL) 122-123 region in human foreskin fibroblasts and pre-transplant peripheral blood mononuclear cells stimulated with HCMV. The present study aimed to quantitate the expression of Homo sapiens (hsa)-miR-200b-3p and 200c-3p in HCMV-infected tissues. We collected 240 HCMV-infected and 154 HCMV-non-infected, formalin-fixed, paraffin-embedded tissue samples of the gastrointestinal (GI) tract and bronchi/lungs. MiRNAs, HCMV, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were quantitated by quantitative reverse transcription-PCR (qRT-PCR) and quantitative PCR (qPCR) on the basis of standard curves generated using miRNA mimics, the HCMV strain from National Institute for Biological Standards and Control (NIBSC) 09/162, and GAPDH control. To avoid the effect of cell counts on the qRT-PCR and qPCR results, the data were normalized to GAPDH levels. HCMV-infected tissues had significantly lower levels of 200b-3p/GAPDH (3.03 ± 1.50 compared with 3.98 ± 1.08 log10 copies/μl, P<0.001) and 200c-3p/GAPDH (4.67 ± 1.84 compared with 6.35 ± 1.47 log10 copies/μl, P<0.001) than normal tissues. The values for 200b-3p/GAPDH (r = -0.51, P<0.001) and 200c-3p/GAPDH (r = -0.54, P<0.001) were significantly inversely correlated with HCMV load. Low tissue levels of 200b-3p and 200c-3p in humans are associated with cytopathic inflammation due to HCMV infection.-
dc.description.statementOfResponsibilityrestriction-
dc.languageEngland-
dc.publisher1573-4935-
dc.relation.isPartOfBioscience Reports-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleExpression of human miR-200b-3p and -200c-3p in cytomegalovirus-infected tissues-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Internal Medicine (내과학교실)-
dc.contributor.googleauthorKyoung Hwa Lee-
dc.contributor.googleauthorBeom Jin Lim-
dc.contributor.googleauthorVictor H. Ferreira-
dc.contributor.googleauthorSeo Yeon Min-
dc.contributor.googleauthorYeon-Mi Hong-
dc.contributor.googleauthorJeong-Hyeon Jo-
dc.contributor.googleauthorSang Hoon Han-
dc.identifier.doi10.1042/BSR20180961-
dc.contributor.localIdA04620-
dc.contributor.localIdA03363-
dc.contributor.localIdA03363-
dc.contributor.localIdA04286-
dc.contributor.localIdA04286-
dc.relation.journalcodeJ03560-
dc.identifier.eissn0144-8463-
dc.identifier.pmid30366960-
dc.identifier.urlhttp://www.bioscirep.org/content/38/6/BSR20180961.long-
dc.subject.keywordcytomegalovirus-
dc.subject.keywordimmediate early protein 2-
dc.subject.keywordmicroRNA-
dc.subject.keywordtissues-
dc.contributor.alternativeNameLee, Kyoung Hwa-
dc.contributor.affiliatedAuthor이경화-
dc.contributor.affiliatedAuthor임범진-
dc.contributor.affiliatedAuthor임범진-
dc.contributor.affiliatedAuthor한상훈-
dc.contributor.affiliatedAuthor한상훈-
dc.citation.volume38-
dc.citation.number6-
dc.citation.startPageBSR20180961-
dc.identifier.bibliographicCitationBioscience Reports, Vol.38(6) : BSR20180961, 2018-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.