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Peptide Nucleic Acid Probe-Based Analysis as a New Detection Method for Clarithromycin Resistance in Helicobacter pylori

DC FieldValueLanguage
dc.contributor.author김지현-
dc.contributor.author송영구-
dc.contributor.author이경화-
dc.contributor.author정다현-
dc.contributor.author정수진-
dc.date.accessioned2019-01-15T16:54:47Z-
dc.date.available2019-01-15T16:54:47Z-
dc.date.issued2018-
dc.identifier.issn1976-2283-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/166712-
dc.description.abstractBackground/Aims: Helicobacter pylori eradication rates are decreasing because of increases in clarithromycin resistance. Thus, finding an easy and accurate method of detecting clarithromycin resistance is important. Methods: We evaluated 70 H. pylori isolates from Korean patients. Dual-labeled peptide nucleic acid (PNA) probes were designed to detect resistance associated with point mutations in 23S ribosomal ribonucleic acid gene domain V (A2142G, A2143G, and T2182C). Data were analyzed by probe-based fluorescence melting curve analysis based on probe-target dissociation temperatures and compared with Sanger sequencing. Results: Among 70 H. pylori isolates, 0, 16, and 58 isolates contained A2142G, A2143G, and T2182C mutations, respectively. PNA probe-based analysis exhibited 100.0% positive predictive values for A2142G and A2143G and a 98.3% positive predictive value for T2182C. PNA probe-based analysis results correlated with 98.6% of Sanger sequencing results (κ-value=0.990; standard error, 0.010). Conclusions: H. pylori clarithromycin resistance can be easily and accurately assessed by dual-labeled PNA probe-based melting curve analysis if probes are used based on the appropriate resistance-related mutations. This method is fast, simple, accurate, and adaptable for clinical samples. It may help clinicians choose a precise eradication regimen.-
dc.description.statementOfResponsibilityopen-
dc.languageEnglish-
dc.publisherEditorial Office of Gut and Liver-
dc.relation.isPartOfGUT AND LIVER-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titlePeptide Nucleic Acid Probe-Based Analysis as a New Detection Method for Clarithromycin Resistance in Helicobacter pylori-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Internal Medicine (내과학교실)-
dc.contributor.googleauthorDa Hyun Jung-
dc.contributor.googleauthorJie-Hyun Kim-
dc.contributor.googleauthorSu Jin Jeong-
dc.contributor.googleauthorSoon Young Park-
dc.contributor.googleauthorIl-Mo Kang-
dc.contributor.googleauthorKyoung Hwa Lee-
dc.contributor.googleauthorYoung Goo Song-
dc.identifier.doi10.5009/gnl18111-
dc.contributor.localIdA00996-
dc.contributor.localIdA02037-
dc.contributor.localIdA02037-
dc.contributor.localIdA04620-
dc.contributor.localIdA04620-
dc.contributor.localIdA03591-
dc.contributor.localIdA03591-
dc.contributor.localIdA03638-
dc.contributor.localIdA03638-
dc.relation.journalcodeJ00954-
dc.identifier.eissn2005-1212-
dc.identifier.pmid30037168-
dc.subject.keywordClarithromycin-
dc.subject.keywordHelicobacter pylori-
dc.subject.keywordMelting array-
dc.subject.keywordPeptide nucleic acids-
dc.contributor.alternativeNameKim, Jie-Hyun-
dc.contributor.affiliatedAuthor김지현-
dc.contributor.affiliatedAuthor송영구-
dc.contributor.affiliatedAuthor송영구-
dc.contributor.affiliatedAuthor이경화-
dc.contributor.affiliatedAuthor이경화-
dc.contributor.affiliatedAuthor정다현-
dc.contributor.affiliatedAuthor정다현-
dc.contributor.affiliatedAuthor정수진-
dc.contributor.affiliatedAuthor정수진-
dc.citation.volume12-
dc.citation.number6-
dc.citation.startPage641-
dc.citation.endPage647-
dc.identifier.bibliographicCitationGUT AND LIVER, Vol.12(6) : 641-647, 2018-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers

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