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Proinflammatory cytokine and nitric oxide production by human macrophages stimulated with Trichomonas vaginalis

DC FieldValueLanguage
dc.contributor.author박순정-
dc.date.accessioned2018-12-06T16:40:53Z-
dc.date.available2018-12-06T16:40:53Z-
dc.date.issued2009-
dc.identifier.issn0023-4001-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/166052-
dc.description.abstractTrichomonas vaginalis commonly causes vaginitis and perhaps cervicitis in women and urethritis in men and women. Macrophages are important immune cells in response to T. vaginalis infection. In this study, we investigated whether human macrophages could be involved in inflammation induced by T. vaginalis. Human monocyte-derived macrophages (HMDM) were co-cultured with T. vaginalis. Live, opsonized-live trichomonads, and T. vaginalis lysates increased proinflammatory cytokines, such as TNF-alpha, IL-1beta, and IL-6 by HMDM. The involvement of nuclear factor (NF)-kappaB signaling pathway in cytokine production induced by T. vaginalis was confirmed by phosphorylation and nuclear translocation of p65 NF-kappaB. In addition, stimulation with live T. vaginalis induced marked augmentation of nitric oxide (NO) production and expression of inducible NO synthase (iNOS) levels in HMDM. However, trichomonad-induced NF-kappaB activation and TNF-alpha production in macrophages were significantly inhibited by inhibition of iNOS levels with L-NMMA (NO synthase inhibitor). Moreover, pretreatment with NF-kappaB inhibitors (PDTC or Bay11-7082) caused human macrophages to produce less TNF-alpha. These results suggest that T. vaginalis stimulates human macrophages to produce proinflammatory cytokines, such as IL-1, IL-6, and TNF-alpha, and NO. In particular, we showed that T. vaginalis induced TNF-alpha production in macrophages through NO-dependent activation of NF-kappaB, which might be closely involved in inflammation caused by T. vaginalis.-
dc.description.statementOfResponsibilityopen-
dc.formatapplication/pdf-
dc.languageEnglish, Korean-
dc.publisherKorean Society for Parasitology-
dc.relation.isPartOfKOREAN JOURNAL OF PARASITOLOGY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleProinflammatory cytokine and nitric oxide production by human macrophages stimulated with Trichomonas vaginalis-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Environmental Medical Biology (환경의생물학교실)-
dc.contributor.googleauthorIk-Hwan Han-
dc.contributor.googleauthorSung Young Goo-
dc.contributor.googleauthorSoon-Jung Park-
dc.contributor.googleauthorSe-Jin Hwang-
dc.contributor.googleauthorYong-Seok Kim-
dc.contributor.googleauthorMichael Sungwoo Yang-
dc.contributor.googleauthorMyoung-Hee Ahn-
dc.contributor.googleauthorJae-Sook Ryu-
dc.identifier.doi10.3347/kjp.2009.47.3.205-
dc.contributor.localIdA01545-
dc.relation.journalcodeJ01957-
dc.identifier.eissn1738-0006-
dc.identifier.pmid19724692-
dc.subject.keywordNF-κB-
dc.subject.keywordTrichomonas vaginalis-
dc.subject.keywordhuman monocyte-derived macrophage-
dc.subject.keywordiNOS-
dc.subject.keywordnitric oxide-
dc.subject.keywordproinflammatory cytokine-
dc.contributor.alternativeNamePark, Soon Jung-
dc.contributor.affiliatedAuthor박순정-
dc.citation.volume47-
dc.citation.number3-
dc.citation.startPage205-
dc.citation.endPage212-
dc.identifier.bibliographicCitationKOREAN JOURNAL OF PARASITOLOGY, Vol.47(3) : 205-212, 2009-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers

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