Cited 1099 times in
Identification of distinct nanoparticles and subsets of extracellular vesicles by asymmetric flow field-flow fractionation
DC Field | Value | Language |
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dc.contributor.author | 김한상 | - |
dc.date.accessioned | 2018-11-29T16:56:26Z | - |
dc.date.available | 2018-11-29T16:56:26Z | - |
dc.date.issued | 2018 | - |
dc.identifier.issn | 1465-7392 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/165929 | - |
dc.description.abstract | The heterogeneity of exosomal populations has hindered our understanding of their biogenesis ; molecular composition ; biodistribution and functions. By employing asymmetric flow field-flow fractionation (AF4) ; we identified two exosome subpopulations (large exosome vesicles ; Exo-L ; 90-120 nm; small exosome vesicles ; Exo-S ; 60-80 nm) and discovered an abundant population of non-membranous nanoparticles termed 'exomeres' (~35 nm). Exomere proteomic profiling revealed an enrichment in metabolic enzymes and hypoxia ; microtubule and coagulation proteins as well as specific pathways ; such as glycolysis and mTOR signalling. Exo-S and Exo-L contained proteins involved in endosomal function and secretion pathways ; and mitotic spindle and IL-2/STAT5 signalling pathways ; respectively. Exo-S ; Exo-L and exomeres each had unique N-glycosylation ; protein ; lipid ; DNA and RNA profiles and biophysical properties. These three nanoparticle subsets demonstrated diverse organ biodistribution patterns ; suggesting distinct biological functions. This study demonstrates that AF4 can serve as an improved analytical tool for isolating extracellular vesicles and addressing the complexities of heterogeneous nanoparticle subpopulations. | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/pdf | - |
dc.language | English | - |
dc.publisher | Macmillan Magazines Ltd. | - |
dc.relation.isPartOf | NATURE CELL BIOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Identification of distinct nanoparticles and subsets of extracellular vesicles by asymmetric flow field-flow fractionation | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학교실) | - |
dc.contributor.googleauthor | Haiying Zhang | - |
dc.contributor.googleauthor | Daniela Freitas | - |
dc.contributor.googleauthor | Han Sang Kim | - |
dc.contributor.googleauthor | Kristina Fabijanic | - |
dc.contributor.googleauthor | Zhong Li | - |
dc.contributor.googleauthor | Haiyan Chen | - |
dc.contributor.googleauthor | Milica Tesic Mark | - |
dc.contributor.googleauthor | Henrik Molina | - |
dc.contributor.googleauthor | Alberto Benito Martin | - |
dc.contributor.googleauthor | Linda Bojmar | - |
dc.contributor.googleauthor | Justin Fang | - |
dc.contributor.googleauthor | Sham Rampersaud | - |
dc.contributor.googleauthor | Ayuko Hoshino | - |
dc.contributor.googleauthor | Irina Matei | - |
dc.contributor.googleauthor | Candia M. Kenific | - |
dc.contributor.googleauthor | Miho Nakajima | - |
dc.contributor.googleauthor | Anders Peter Mutvei | - |
dc.contributor.googleauthor | Pasquale Sansone | - |
dc.contributor.googleauthor | Weston Buehring | - |
dc.contributor.googleauthor | Huajuan Wang | - |
dc.contributor.googleauthor | Juan Pablo Jimenez | - |
dc.contributor.googleauthor | Leona Cohen-Gould | - |
dc.contributor.googleauthor | Navid Paknejad | - |
dc.contributor.googleauthor | Matthew Brendel | - |
dc.contributor.googleauthor | Katia Manova-Todorova | - |
dc.contributor.googleauthor | Ana Magalhães | - |
dc.contributor.googleauthor | José Alexandre Ferreira | - |
dc.contributor.googleauthor | Hugo Osório | - |
dc.contributor.googleauthor | André M. Silva | - |
dc.contributor.googleauthor | Ashish Massey | - |
dc.contributor.googleauthor | Juan R. Cubillos-Ruiz | - |
dc.contributor.googleauthor | Giuseppe Galletti | - |
dc.contributor.googleauthor | Paraskevi Giannakakou | - |
dc.contributor.googleauthor | Ana Maria Cuervo | - |
dc.contributor.googleauthor | John Blenis | - |
dc.contributor.googleauthor | Robert Schwartz | - |
dc.contributor.googleauthor | Mary Sue Brady | - |
dc.contributor.googleauthor | Héctor Peinado | - |
dc.contributor.googleauthor | Jacqueline Bromberg | - |
dc.contributor.googleauthor | Hiroshi Matsui | - |
dc.contributor.googleauthor | Celso A. Reis | - |
dc.contributor.googleauthor | David Lyden | - |
dc.identifier.doi | 10.1038/s41556-018-0040-4 | - |
dc.contributor.localId | A01098 | - |
dc.relation.journalcode | J02291 | - |
dc.identifier.eissn | 1476-4679 | - |
dc.identifier.pmid | 29459780 | - |
dc.contributor.alternativeName | Kim ; Han Sang | - |
dc.contributor.affiliatedAuthor | 김한상 | - |
dc.citation.volume | 20 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 332 | - |
dc.citation.endPage | 343 | - |
dc.identifier.bibliographicCitation | NATURE CELL BIOLOGY, Vol.20(3) : 332-343, 2018 | - |
dc.identifier.rimsid | 60314 | - |
dc.type.rims | ART | - |
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