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Identification of critical amino acids in the proximal C-terminal of TREK-2 K+ channel for activation by acidic pHi and ATP-dependent inhibition

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dc.contributor.author신동훈-
dc.date.accessioned2018-11-26T05:56:18Z-
dc.date.available2018-11-26T05:56:18Z-
dc.date.issued2018-
dc.identifier.issn0031-6768-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/165825-
dc.description.abstractTWIK-related two-pore domain K+ channels (TREKs) are regulated by intracellular pH (pHi) and Phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2). Previously, Glu306 in proximal C-terminal (pCt) of mouse TREK-1 was identified as the pHi-sensing residue. The direction of PI(4,5)P2 sensitivity is controversial, and we have recently shown that TREKs are inhibited by intracellular ATP via endogenous PI(4,5)P2 formation. Here we investigate the anionic and cationic residues of pCt for the pHi and ATP-sensitivity in human TREK-2 (hTREK-2). In inside-out patch clamp recordings (ITREK-2,i-o), acidic pHi-induced activation was absent in E332A and was partly attenuated in E335A. Neutralization of cationic Lys (K330A) also eliminated the acidic pHi sensitivity of ITREK-2,i-o. Unlike the inhibition of wild-type (WT) ITREK-2,i-o by intracellular ATP, neither E332A nor K330A was sensitive to ATP. Nevertheless, exogenous PI(4,5)P2 (10 μM) abolished ITREK-2 i-o in all the above mutants as well as in WT, indicating unspecific inhibition by exogenous PI(4,5)P2. In whole-cell recordings of TREK-2 (ITREK-2,w-c), K330A and E332A showed higher or fully active basal activity, showing attenuated or insignificant activation by 2-APB, arachidonic acid, or acidic pHe 6.9. ITREK-1,w-c of WT is largely suppressed by pHe 6.9, and the inhibition is slightly attenuated in K312A and E315A. The results show concerted roles of the oppositely charged Lys and Glu in pCt for the ATP-dependent low basal activity and pHi sensitivity.-
dc.description.statementOfResponsibilityrestriction-
dc.languageGerman, English-
dc.publisherSpringer-
dc.relation.isPartOfPflugers Archiv - European Journal of Physiology-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleIdentification of critical amino acids in the proximal C-terminal of TREK-2 K+ channel for activation by acidic pHi and ATP-dependent inhibition-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentBioMedical Science Institute (의생명과학부)-
dc.contributor.googleauthorJoohan Woo-
dc.contributor.googleauthorYoung Keul Jun-
dc.contributor.googleauthorYin-Hua Zhang-
dc.contributor.googleauthorJoo Hyun Nam-
dc.contributor.googleauthorDong Hoon Shin-
dc.contributor.googleauthorSung Joon Kim-
dc.identifier.doi10.1007/s00424-017-2072-1-
dc.contributor.localIdA05644-
dc.relation.journalcodeJ02502-
dc.identifier.eissn1432-2013-
dc.identifier.pmid28988317-
dc.identifier.urlhttps://link.springer.com/article/10.1007%2Fs00424-017-2072-1-
dc.contributor.alternativeNameShin, Dong Hoon::Shin, Dong Hoon::Shin, Dong Hoon::Shin, Dong Hoon-
dc.contributor.affiliatedAuthor신동훈-
dc.citation.volume470-
dc.citation.number2-
dc.citation.startPage327-
dc.citation.endPage337-
dc.identifier.bibliographicCitationPflugers Archiv - European Journal of Physiology, Vol.470(2) : 327-337, 2018-
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers

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