Cited 12 times in
Droplet digital PCR-based EGFR mutation detection with an internal quality control index to determine the quality of DNA.
DC Field | Value | Language |
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dc.contributor.author | 민병소 | - |
dc.date.accessioned | 2018-11-16T16:52:31Z | - |
dc.date.available | 2018-11-16T16:52:31Z | - |
dc.date.issued | 2018 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/165419 | - |
dc.description.abstract | In clinical translational research and molecular in vitro diagnostics, a major challenge in the detection of genetic mutations is overcoming artefactual results caused by the low-quality of formalin-fixed paraffin-embedded tissue (FFPET)-derived DNA (FFPET-DNA). Here, we propose the use of an 'internal quality control (iQC) index' as a criterion for judging the minimum quality of DNA for PCR-based analyses. In a pre-clinical study comparing the results from droplet digital PCR-based EGFR mutation test (ddEGFR test) and qPCR-based EGFR mutation test (cobas EGFR test), iQC index ≥ 0.5 (iQC copies ≥ 500, using 3.3 ng of FFPET-DNA [1,000 genome equivalents]) was established, indicating that more than half of the input DNA was amplifiable. Using this criterion, we conducted a retrospective comparative clinical study of the ddEGFR and cobas EGFR tests for the detection of EGFR mutations in non-small cell lung cancer (NSCLC) FFPET-DNA samples. Compared with the cobas EGFR test, the ddEGFR test exhibited superior analytical performance and equivalent or higher clinical performance. Furthermore, iQC index is a reliable indicator of the quality of FFPET-DNA and could be used to prevent incorrect diagnoses arising from low-quality samples. | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/pdf | - |
dc.language | English | - |
dc.publisher | Nature Publishing Group | - |
dc.relation.isPartOf | SCIENTIFIC REPORTS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Droplet digital PCR-based EGFR mutation detection with an internal quality control index to determine the quality of DNA. | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Surgery (외과학교실) | - |
dc.contributor.googleauthor | Sung-Su Kim | - |
dc.contributor.googleauthor | Hyun-Jeung Choi | - |
dc.contributor.googleauthor | Jin Ju Kim | - |
dc.contributor.googleauthor | M. Sun Kim | - |
dc.contributor.googleauthor | In-Seon Lee | - |
dc.contributor.googleauthor | Bohyun Byun | - |
dc.contributor.googleauthor | Lina Jia | - |
dc.contributor.googleauthor | Myung Ryurl Oh | - |
dc.contributor.googleauthor | Youngho Moon | - |
dc.contributor.googleauthor | Sarah Park | - |
dc.contributor.googleauthor | Joon-Seok Choi | - |
dc.contributor.googleauthor | Seoung Wan Chae | - |
dc.contributor.googleauthor | Byung-Ho Nam | - |
dc.contributor.googleauthor | Jin-Soo Kim | - |
dc.contributor.googleauthor | Jihun Kim | - |
dc.contributor.googleauthor | Byung Soh Min | - |
dc.contributor.googleauthor | Jae Seok Lee | - |
dc.contributor.googleauthor | Jae-Kyung Won | - |
dc.contributor.googleauthor | Soo Youn Cho | - |
dc.contributor.googleauthor | Yoon-La Choi | - |
dc.contributor.googleauthor | Young Kee Shin | - |
dc.identifier.doi | 10.1038/s41598-017-18642-x | - |
dc.contributor.localId | A01402 | - |
dc.relation.journalcode | J02646 | - |
dc.identifier.eissn | 2045-2322 | - |
dc.identifier.pmid | 29323170 | - |
dc.contributor.alternativeName | Min, Byung Soh | - |
dc.contributor.affiliatedAuthor | 민병소 | - |
dc.citation.volume | 8 | - |
dc.citation.startPage | 543 | - |
dc.identifier.bibliographicCitation | SCIENTIFIC REPORTS, Vol.8 : 543, 2018 | - |
dc.identifier.rimsid | 58884 | - |
dc.type.rims | ART | - |
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