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Enhanced articular cartilage regeneration with SIRT1-activated MSCs using gelatin-based hydrogel.

 Seong Mi Choi  ;  Kyoung-Mi Lee  ;  Seung Bae Ryu  ;  Yoo Jung Park  ;  Yeok Gu Hwang  ;  Dawoon Baek  ;  Yoorim Choi  ;  Kwang Hwan Park  ;  Ki Dong Park  ;  Jin Woo Lee 
 Cell Death & Disease, Vol.9(9) : 866, 2018 
Journal Title
 Cell Death & Disease 
Issue Date
To investigate the functional effects of resveratrol (RSV) on mesenchymal stem cells (MSCs), we treated MSCs with RSV continuously during ex vivo expansion. MSCs were continuously treated with RSV from passage (P) 0 to P5. A proliferative capacity of RSV-treated MSCs was higher than that of non-treated MSCs and similar with P1-MSCs. Continuous treatment of RSV on MSCs increased the stemness and inhibited the senescence. During chondrogenic differentiation in vitro, RSV-treated MSCs had higher differentiation potential and reduced hypertrophic maturation, which are limitations for hyaline cartilage formation. The histological analysis of micromass demonstrated increased chondrogenic differentiation potential. We further explored the therapeutic effectiveness of this method in a rabbit osteochondral defect model. A rabbit osteochondral defect model was established to investigate the hyaline cartilage regeneration potential of RSV-treated MSCs. Moreover, the cartilage regeneration potential of RSV-treated MSCs was greater than that of untreated MSCs. The expression levels of chondrogenic markers increased and those of hypertrophic markers decreased in RSV-treated MSCs compared with untreated MSCs. Sustained treatment of RSV on MSCs during ex vivo expansion resulted in the maintenance of stemness and enhanced chondrogenic differentiation potential. Consequentially, highly efficient MSCs promoted superior hyaline cartilage regeneration in vivo. This novel treatment method provides a basis for cell-based tissue engineering.
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1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부)
Yonsei Authors
박광환(Park, Kwang Hwan) ORCID logo https://orcid.org/0000-0002-2110-0559
박유정(Park, Yoo Jung)
백다운(Baek, Dawoon) ORCID logo https://orcid.org/0000-0002-3794-6153
이경미(Lee, Kyoung Mi) ORCID logo https://orcid.org/0000-0002-9038-8162
이진우(Lee, Jin Woo) ORCID logo https://orcid.org/0000-0002-0293-9017
황역구(Hwang, Yeokgu)
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