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Enhanced articular cartilage regeneration with SIRT1-activated MSCs using gelatin-based hydrogel.

Authors
 Seong Mi Choi  ;  Kyoung-Mi Lee  ;  Seung Bae Ryu  ;  Yoo Jung Park  ;  Yeok Gu Hwang  ;  Dawoon Baek  ;  Yoorim Choi  ;  Kwang Hwan Park  ;  Ki Dong Park  ;  Jin Woo Lee 
Citation
 CELL DEATH & DISEASE, Vol.9(9) : 866, 2018 
Journal Title
 CELL DEATH & DISEASE 
Issue Date
2018
Abstract
To investigate the functional effects of resveratrol (RSV) on mesenchymal stem cells (MSCs), we treated MSCs with RSV continuously during ex vivo expansion. MSCs were continuously treated with RSV from passage (P) 0 to P5. A proliferative capacity of RSV-treated MSCs was higher than that of non-treated MSCs and similar with P1-MSCs. Continuous treatment of RSV on MSCs increased the stemness and inhibited the senescence. During chondrogenic differentiation in vitro, RSV-treated MSCs had higher differentiation potential and reduced hypertrophic maturation, which are limitations for hyaline cartilage formation. The histological analysis of micromass demonstrated increased chondrogenic differentiation potential. We further explored the therapeutic effectiveness of this method in a rabbit osteochondral defect model. A rabbit osteochondral defect model was established to investigate the hyaline cartilage regeneration potential of RSV-treated MSCs. Moreover, the cartilage regeneration potential of RSV-treated MSCs was greater than that of untreated MSCs. The expression levels of chondrogenic markers increased and those of hypertrophic markers decreased in RSV-treated MSCs compared with untreated MSCs. Sustained treatment of RSV on MSCs during ex vivo expansion resulted in the maintenance of stemness and enhanced chondrogenic differentiation potential. Consequentially, highly efficient MSCs promoted superior hyaline cartilage regeneration in vivo. This novel treatment method provides a basis for cell-based tissue engineering.
Files in This Item:
T201803918.pdf Download
DOI
10.1038/s41419-018-0914-1
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
Yonsei Authors
Park, Kwang Hwan(박광환) ORCID logo https://orcid.org/0000-0002-2110-0559
Park, Yoo Jung(박유정)
Baek, Dawoon(백다운) ORCID logo https://orcid.org/0000-0002-3794-6153
Lee, Kyoung Mi(이경미) ORCID logo https://orcid.org/0000-0002-9038-8162
Lee, Jin Woo(이진우) ORCID logo https://orcid.org/0000-0002-0293-9017
Hwang, Yeokgu(황역구)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/165383
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