Cited 5 times in
Differences in Colistin-resistant Acinetobacter baumannii Clinical Isolates Between Patients With and Without Prior Colistin Treatment.
DC Field | Value | Language |
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dc.contributor.author | 김도균 | - |
dc.contributor.author | 용동은 | - |
dc.contributor.author | 이혁민 | - |
dc.contributor.author | 정석훈 | - |
dc.contributor.author | 홍준성 | - |
dc.date.accessioned | 2018-11-16T16:40:26Z | - |
dc.date.available | 2018-11-16T16:40:26Z | - |
dc.date.issued | 2018 | - |
dc.identifier.issn | 2234-3806 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/165209 | - |
dc.description.abstract | BACKGROUND: The increasing morbidity and mortality rates associated with Acinetobacter baumannii are due to the emergence of drug resistance and the limited treatment options. We compared characteristics of colistin-resistant Acinetobacter baumannii (CR-AB) clinical isolates recovered from patients with and without prior colistin treatment. We assessed whether prior colistin treatment affects the resistance mechanism of CR-AB isolates, mortality rates, and clinical characteristics. Additionally, a proper method for identifying CR-AB was determined. METHODS: We collected 36 non-duplicate CR-AB clinical isolates resistant to colistin. Antimicrobial susceptibility testing, Sanger sequencing analysis, molecular typing, lipid A structure analysis, and in vitro synergy testing were performed. Eleven colistin-susceptible AB isolates were used as controls. RESULTS: Despite no differences in clinical characteristics between patients with and without prior colistin treatment, resistance-causing genetic mutations were more frequent in isolates from colistin-treated patients. Distinct mutations were overlooked via the Sanger sequencing method, perhaps because of a masking effect by the colistin-susceptible AB subpopulation of CR-AB isolates lacking genetic mutations. However, modified lipid A analysis revealed colistin resistance peaks, despite the population heterogeneity, and peak levels were significantly different between the groups. CONCLUSIONS: Although prior colistin use did not induce clinical or susceptibility differences, we demonstrated that identification of CR-AB by sequencing is insufficient. We propose that population heterogeneity has a masking effect, especially in colistin non-treated patients; therefore, accurate testing methods reflecting physiological alterations of the bacteria, such as phosphoethanolamine-modified lipid A identification by matrix-assisted laser desorption ionization-time of flight, should be employed. | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/pdf | - |
dc.language | English | - |
dc.publisher | Korean Society for Laboratory Medicine | - |
dc.relation.isPartOf | ANNALS OF LABORATORY MEDICINE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Acinetobacter Infections/drug therapy* | - |
dc.subject.MESH | Acinetobacter Infections/microbiology | - |
dc.subject.MESH | Acinetobacter baumannii/drug effects | - |
dc.subject.MESH | Acinetobacter baumannii/isolation & purification* | - |
dc.subject.MESH | Adolescent | - |
dc.subject.MESH | Adult | - |
dc.subject.MESH | Aged | - |
dc.subject.MESH | Anti-Bacterial Agents/therapeutic use* | - |
dc.subject.MESH | Anti-Infective Agents/pharmacology | - |
dc.subject.MESH | Colistin/therapeutic use* | - |
dc.subject.MESH | DNA, Bacterial/genetics | - |
dc.subject.MESH | DNA, Bacterial/metabolism | - |
dc.subject.MESH | Drug Resistance, Bacterial/genetics | - |
dc.subject.MESH | Electrophoresis, Gel, Pulsed-Field | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Lipid A/analysis | - |
dc.subject.MESH | Lipid A/chemistry | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Microbial Sensitivity Tests | - |
dc.subject.MESH | Middle Aged | - |
dc.subject.MESH | Multilocus Sequence Typing | - |
dc.subject.MESH | Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization | - |
dc.subject.MESH | Young Adult | - |
dc.title | Differences in Colistin-resistant Acinetobacter baumannii Clinical Isolates Between Patients With and Without Prior Colistin Treatment. | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Laboratory Medicine (진단검사의학교실) | - |
dc.contributor.googleauthor | Yu Jin Park | - |
dc.contributor.googleauthor | Duck Jin Hong | - |
dc.contributor.googleauthor | Eun-Jeong Yoon | - |
dc.contributor.googleauthor | Dokyun Kim | - |
dc.contributor.googleauthor | Min Hyuk Choi | - |
dc.contributor.googleauthor | Jun Sung Hong | - |
dc.contributor.googleauthor | Hyukmin Lee | - |
dc.contributor.googleauthor | Dongeun Yong | - |
dc.contributor.googleauthor | Seok Hoon Jeong | - |
dc.identifier.doi | 10.3343/alm.2018.38.6.545 | - |
dc.contributor.localId | A04891 | - |
dc.contributor.localId | A02423 | - |
dc.contributor.localId | A03286 | - |
dc.contributor.localId | A03619 | - |
dc.relation.journalcode | J00164 | - |
dc.identifier.eissn | 2234-3814 | - |
dc.identifier.pmid | 30027698 | - |
dc.subject.keyword | Acinetobacter baumannii | - |
dc.subject.keyword | Colistin | - |
dc.subject.keyword | Lipid A analysis | - |
dc.subject.keyword | Pathogenesis | - |
dc.subject.keyword | Population heterogeneity | - |
dc.subject.keyword | Resistance | - |
dc.contributor.alternativeName | Kim, Dokyun | - |
dc.contributor.alternativeName | Yong, Dong Eun | - |
dc.contributor.alternativeName | Lee, Hyuk Min | - |
dc.contributor.alternativeName | Jeong, Seok Hoon | - |
dc.contributor.affiliatedAuthor | 김도균 | - |
dc.contributor.affiliatedAuthor | 용동은 | - |
dc.contributor.affiliatedAuthor | 이혁민 | - |
dc.contributor.affiliatedAuthor | 정석훈 | - |
dc.citation.volume | 38 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 545 | - |
dc.citation.endPage | 554 | - |
dc.identifier.bibliographicCitation | ANNALS OF LABORATORY MEDICINE, Vol.38(6) : 545-554, 2018 | - |
dc.identifier.rimsid | 58627 | - |
dc.type.rims | ART | - |
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