Cited 4 times in
O-deGlcNAcylation is required for Entamoeba histolytica-induced HepG2 cell death
DC Field | Value | Language |
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dc.contributor.author | 민아림 | - |
dc.contributor.author | 신명헌 | - |
dc.contributor.author | 이영아 | - |
dc.date.accessioned | 2018-11-16T16:40:10Z | - |
dc.date.available | 2018-11-16T16:40:10Z | - |
dc.date.issued | 2018 | - |
dc.identifier.issn | 0882-4010 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/165204 | - |
dc.description.abstract | Entamoeba histolytica is an enteric tissue-invading protozoan parasite that causes amoebic colitis and occasionally liver abscess in humans. E. histolytica can induce host-cell apoptosis by initiating various intracellular signaling mechanisms closely associated with tissue pathogenesis and parasitic immune evasion. O-GlcNAcylation, similar to phosphorylation, is involved in various cell-signaling processes, including apoptosis and proliferation, with O-GlcNAc addition and removal regulated by O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA), respectively. However, whether O-GlcNAc alterations in host cells affect E. histolytica-induced cell death and which signal molecules participate in E. histolytica-induced deglycosylation remain unknown. In this study, co-incubation of HepG2 cells with E. histolytica increased DNA fragmentation and LDH release as compared with control cells. Additionally, Gal-lectin-mediated amoebic adherence of live trophozoites to HepG2 cells decreased O-GlcNAcylated protein levels within 5 min. We also observed a rapid decrease in cellular OGT protein level, but not OGA, in HepG2 cells in a contact-dependent manner. Furthermore, HepG2 pretreatment with OGA inhibitors or OGA siRNA prevented E. histolytica-induced O-deGlcNAcylation, DNA fragmentation, and LDH release. Our results suggested that E. histolytica-induced O-deGlcNAcylation in HepG2 cells was an important process required for hepatocyte cell death induced by E. histolytica adherence. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Academic Press | - |
dc.relation.isPartOf | MICROBIAL PATHOGENESIS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | O-deGlcNAcylation is required for Entamoeba histolytica-induced HepG2 cell death | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Tropica Medicine (열대의학교실) | - |
dc.contributor.googleauthor | Young Ah Lee | - |
dc.contributor.googleauthor | Arim Min | - |
dc.contributor.googleauthor | Myeong Heon Shin | - |
dc.identifier.doi | 10.1016/j.micpath.2018.07.012 | - |
dc.contributor.localId | A01405 | - |
dc.contributor.localId | A02099 | - |
dc.contributor.localId | A02959 | - |
dc.relation.journalcode | J02230 | - |
dc.identifier.eissn | 1096-1208 | - |
dc.identifier.pmid | 30016682 | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S088240101830175X | - |
dc.subject.keyword | Entamoeba histolytica | - |
dc.subject.keyword | O-GlcNAc | - |
dc.subject.keyword | Cell death | - |
dc.subject.keyword | OGT | - |
dc.subject.keyword | OGA | - |
dc.contributor.alternativeName | Min, A Rim | - |
dc.contributor.alternativeName | Shin, Myeong Heon | - |
dc.contributor.alternativeName | Lee, Young Ah | - |
dc.contributor.affiliatedAuthor | 민아림 | - |
dc.contributor.affiliatedAuthor | 신명헌 | - |
dc.contributor.affiliatedAuthor | 이영아 | - |
dc.citation.volume | 123 | - |
dc.citation.startPage | 285 | - |
dc.citation.endPage | 295 | - |
dc.identifier.bibliographicCitation | MICROBIAL PATHOGENESIS, Vol.123 : 285-295, 2018 | - |
dc.identifier.rimsid | 58622 | - |
dc.type.rims | ART | - |
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