Cited 58 times in
Extracellular Vesicles Derived from Hypoxic Human Mesenchymal Stem Cells Attenuate GSK3β Expression via miRNA-26a in an Ischemia-Reperfusion Injury Model.
DC Field | Value | Language |
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dc.contributor.author | 이승현 | - |
dc.contributor.author | 정보영 | - |
dc.date.accessioned | 2018-09-28T08:51:32Z | - |
dc.date.available | 2018-09-28T08:51:32Z | - |
dc.date.issued | 2018 | - |
dc.identifier.issn | 0513-5796 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/163164 | - |
dc.description.abstract | PURPOSE: Bioactive molecules critical to intracellular signaling are contained in extracellular vesicles (EVs) and have cardioprotective effects in ischemia/reperfusion (IR) injured hearts. This study investigated the mechanism of the cardioprotective effects of EVs derived from hypoxia-preconditioned human mesenchymal stem cells (MSCs). MATERIALS AND METHODS: EV solutions (0.4 μg/μL) derived from normoxia-preconditioned MSCs (EV(NM)) and hypoxia-preconditioned MSCs (EV(HM)) were delivered in a rat IR injury model. Successful EV delivery was confirmed by the detection of PKH26 staining in hearts from EV-treated rats. RESULTS: EV(HM) significantly reduced infarct size (24±2% vs. 8±1%, p<0.001), and diminished arrhythmias by recovering electrical conduction, I(Na) current, and Cx43 expression. EV(HM) also reversed reductions in Wnt1 and β-catenin levels and increases in GSK3β induced after IR injury. miRNA-26a was significantly increased in EV(HM), compared with EV(NM), in real-time PCR. Finally, in in vitro experiments, hypoxia-induced increases in GSK3β expression were significantly reduced by the overexpression of miRNA-26a. CONCLUSION: EV(HM) reduced IR injury by suppressing GSK3β expression via miRNA-26a and increased Cx43 expression. These findings suggest that the beneficial effect of EVHM is related with Wnt signaling pathway. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Yonsei University | - |
dc.relation.isPartOf | YONSEI MEDICAL JOURNAL | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Connexin 43 | - |
dc.subject.MESH | Extracellular Vesicles | - |
dc.subject.MESH | Glycogen Synthase Kinase 3 beta/antagonists & inhibitors | - |
dc.subject.MESH | Glycogen Synthase Kinase 3 beta/genetics | - |
dc.subject.MESH | Glycogen Synthase Kinase 3 beta/metabolism* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Hypoxia/metabolism | - |
dc.subject.MESH | Hypoxia/physiopathology* | - |
dc.subject.MESH | Immunohistochemistry | - |
dc.subject.MESH | Ischemia/physiopathology* | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Mesenchymal Stromal Cells/metabolism | - |
dc.subject.MESH | MicroRNAs/antagonists & inhibitors | - |
dc.subject.MESH | MicroRNAs/genetics | - |
dc.subject.MESH | MicroRNAs/metabolism* | - |
dc.subject.MESH | RNA, Small Interfering/metabolism | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Reperfusion Injury* | - |
dc.subject.MESH | beta Catenin | - |
dc.title | Extracellular Vesicles Derived from Hypoxic Human Mesenchymal Stem Cells Attenuate GSK3β Expression via miRNA-26a in an Ischemia-Reperfusion Injury Model. | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine | - |
dc.contributor.department | Dept. of Biochemistry & Molecular Biology | - |
dc.contributor.googleauthor | Hyewon Park | - |
dc.contributor.googleauthor | Hyelim Park | - |
dc.contributor.googleauthor | Dasom Mun | - |
dc.contributor.googleauthor | Jiyoung Kang | - |
dc.contributor.googleauthor | Hyoeun Kim | - |
dc.contributor.googleauthor | Michael Kim | - |
dc.contributor.googleauthor | Shanyu Cui | - |
dc.contributor.googleauthor | Seung-Hyun Lee | - |
dc.contributor.googleauthor | Boyoung Joung | - |
dc.identifier.doi | 10.3349/ymj.2018.59.6.736 | - |
dc.contributor.localId | A02932 | - |
dc.contributor.localId | A03609 | - |
dc.relation.journalcode | J02813 | - |
dc.identifier.eissn | 1976-2437 | - |
dc.identifier.pmid | 29978610 | - |
dc.subject.keyword | Extracellular vesicles | - |
dc.subject.keyword | GSK3β | - |
dc.subject.keyword | arrhythmia | - |
dc.subject.keyword | ischemia reperfusion | - |
dc.subject.keyword | miRNA-26a | - |
dc.contributor.alternativeName | Lee, Seung Hyun | - |
dc.contributor.alternativeName | Joung, Bo Young | - |
dc.contributor.affiliatedAuthor | Lee, Seung Hyun | - |
dc.contributor.affiliatedAuthor | Joung, Bo Young | - |
dc.citation.volume | 59 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 736 | - |
dc.citation.endPage | 745 | - |
dc.identifier.bibliographicCitation | YONSEI MEDICAL JOURNAL, Vol.59(6) : 736-745, 2018 | - |
dc.identifier.rimsid | 58433 | - |
dc.type.rims | ART | - |
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