0 66

Cited 6 times in

MicroRNA-630 inhibitor sensitizes chemoresistant ovarian cancer to chemotherapy by enhancing apoptosis

DC FieldValueLanguage
dc.contributor.author김상운-
dc.contributor.author김성훈-
dc.contributor.author김영태-
dc.contributor.author남은지-
dc.contributor.author어경진-
dc.contributor.author이정윤-
dc.date.accessioned2018-09-28T08:49:38Z-
dc.date.available2018-09-28T08:49:38Z-
dc.date.issued2018-
dc.identifier.issn0006-291X-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/163126-
dc.description.abstractMicroRNA-630 (miR-630) has been implicated in the development and progression of multiple cancers. The current study aimed to investigate the role of miR-630 in chemoresistant epithelial ovarian cancer. MiR-630 expression levels were detected in ovarian cancer cell line SKOV3 and paclitaxel-resistant SKOV3 (SKOV3-TR) via microarray and qRT-PCR. MiR-630 inhibitors and negative controls were transfected into SKOV3 and SKOV3-TR cells. Wound healing, invasion, chemosensitivity, and cell apoptosis assays were performed to determine proliferation and migration rates. Chemoresistant patient-derived xenograft (PDX) models were established and utilized to verify the effect of miR-630 on chemoresistant ovarian cancer. Inhibition of miR-630 decreased cell proliferation and enhanced the sensitivity of SKOV3-TR and SKOV3 cells to paclitaxel. In the chemosensitivity assay, we observed that the miR-630 inhibitor exhibited a synergistic effect with paclitaxel on SKOV3-TR cells. Inhibition was correlated with enhanced expression of apoptosis-related proteins. APAF-1 was predicted to be a potential target of miR-630. An in vivo PDX study showed that the miR-630 inhibitor sensitized chemoresistant ovarian cancer to paclitaxel. Thus, miR-630 inhibitor sensitizes chemoresistant epithelial ovarian cancer to chemotherapy by enhancing apoptosis. Our findings suggest that miR-630 might be a potential therapeutic target for chemotherapy-resistant ovarian cancer.-
dc.description.statementOfResponsibilityrestriction-
dc.languageEnglish-
dc.publisherElsevier-
dc.relation.isPartOfBiochemical and Biophysical Research Communications-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHAntineoplastic Agents, Phytogenic/pharmacology*-
dc.subject.MESHApoptosis/drug effects*-
dc.subject.MESHApoptotic Protease-Activating Factor 1/genetics-
dc.subject.MESHCell Line, Tumor-
dc.subject.MESHCell Proliferation/drug effects-
dc.subject.MESHDrug Resistance, Neoplasm/drug effects*-
dc.subject.MESHFemale-
dc.subject.MESHGene Expression Regulation, Neoplastic/drug effects-
dc.subject.MESHHumans-
dc.subject.MESHMice-
dc.subject.MESHMicroRNAs/antagonists & inhibitors*-
dc.subject.MESHMicroRNAs/genetics-
dc.subject.MESHOvarian Neoplasms/drug therapy*-
dc.subject.MESHOvarian Neoplasms/genetics-
dc.subject.MESHOvarian Neoplasms/pathology-
dc.subject.MESHOvary/drug effects-
dc.subject.MESHOvary/metabolism-
dc.subject.MESHOvary/pathology-
dc.subject.MESHPaclitaxel/pharmacology*-
dc.titleMicroRNA-630 inhibitor sensitizes chemoresistant ovarian cancer to chemotherapy by enhancing apoptosis-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine-
dc.contributor.departmentDept. of Obstetrics & Gynecology-
dc.contributor.googleauthorKyung Jin Eoh-
dc.contributor.googleauthorSo Hyun Lee-
dc.contributor.googleauthorHee Jung Kim-
dc.contributor.googleauthorJung-Yun Lee-
dc.contributor.googleauthorSunghoon Kim-
dc.contributor.googleauthorSang Wun Kim-
dc.contributor.googleauthorYoung Tae Kim-
dc.contributor.googleauthorEun Ji Nam-
dc.identifier.doi10.1016/j.bbrc.2018.02.062-
dc.contributor.localIdA00526-
dc.contributor.localIdA00595-
dc.contributor.localIdA00729-
dc.contributor.localIdA01262-
dc.contributor.localIdA04842-
dc.contributor.localIdA04638-
dc.relation.journalcodeJ00281-
dc.identifier.eissn1090-2104-
dc.identifier.pmid29452092-
dc.identifier.urlhttps://www.sciencedirect.com/science/article/pii/S0006291X18302857-
dc.subject.keywordApoptosis-
dc.subject.keywordMicroRNA-
dc.subject.keywordOvarian cancer-
dc.subject.keywordmiR-630-
dc.contributor.alternativeNameKim, Sang Wun-
dc.contributor.alternativeNameKim, Sung Hoon-
dc.contributor.alternativeNameKim, Young Tae-
dc.contributor.alternativeNameNam, Eun Ji-
dc.contributor.alternativeNameEoh, Kyung Jin-
dc.contributor.alternativeNameLee, Jung-Yun-
dc.contributor.affiliatedAuthorKim, Sang Wun-
dc.contributor.affiliatedAuthorKim, Sung Hoon-
dc.contributor.affiliatedAuthorKim, Young Tae-
dc.contributor.affiliatedAuthorNam, Eun Ji-
dc.contributor.affiliatedAuthorEoh, Kyung Jin-
dc.contributor.affiliatedAuthorLee, Jung-Yun-
dc.citation.volume497-
dc.citation.number2-
dc.citation.startPage513-
dc.citation.endPage520-
dc.identifier.bibliographicCitationBiochemical and Biophysical Research Communications, Vol.497(2) : 513-520, 2018-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Obstetrics and Gynecology (산부인과학교실) > 1. Journal Papers

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.