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Color-coded LED microscopy for quantitative phase imaging: Implementation and application to sperm motility analysis

Authors
 Wonchan Lee  ;  Jun-Ho Choi  ;  Suho Ryu  ;  Daeseong Jung  ;  Jaewoo Song  ;  Jong-Seok Lee  ;  Chulmin Joo 
Citation
 METHODS, Vol.136 : 66-74, 2018 
Journal Title
 METHODS 
ISSN
 1046-2023 
Issue Date
2018
Keywords
Computational imaging ; Microscopy ; Phase retrieval ; Sperm analysis
Abstract
Color-coded light-emitting diode (LED) microscopy (cLEDscope) is a novel computational microscopy technique capable of multi-contrast and quantitative phase imaging of biological specimens using color-multiplexed illumination. Using specially designed LED patterns, it is capable of recording multiple differential phase contrast (DPC) images in a single exposure and employs a computational algorithm to retrieve the phase distribution of the specimens. Herein, we describe the detailed procedures in the cLEDscope implementation for quantitative phase imaging. Several notable features and caveats in the cLEDscope setup and image processing are also outlined. The imaging model is derived for our specific configuration, and the associated phase-retrieval algorithms are presented on the basis of a weak-object transfer function. As an illustrative application of the quantitative cLEDscope, we demonstrate its utility as a sperm-motility analyzer by exploiting its real-time quantitative imaging capability.
Full Text
https://www.sciencedirect.com/science/article/pii/S104620231730172X
DOI
10.1016/j.ymeth.2017.11.010
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Song, Jae Woo(송재우) ORCID logo https://orcid.org/0000-0002-1877-5731
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/162100
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