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Same-Day Identification and Antimicrobial Susceptibility Testing of Bacteria in Positive Blood Culture Broths Using Short-Term Incubation on Solid Medium with the MicroFlex LT, Vitek-MS, and Vitek2 Systems

DC FieldValueLanguage
dc.contributor.author김명숙-
dc.contributor.author용동은-
dc.contributor.author이경원-
dc.contributor.author한금희-
dc.date.accessioned2018-08-28T16:55:08Z-
dc.date.available2018-08-28T16:55:08Z-
dc.date.issued2018-
dc.identifier.issn2234-3806-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/162068-
dc.description.abstractBACKGROUND: Early and appropriate antibiotic treatment improves the clinical outcome of patients with septicemia; therefore, reducing the turn-around time for identification (ID) and antimicrobial susceptibility test (AST) results is essential. We established a method for rapid ID and AST using short-term incubation of positive blood culture broth samples on solid media, and evaluated its performance relative to that of the conventional method using two rapid ID systems and a rapid AST method. METHODS: A total of 254 mono-microbial samples were included. Positive blood culture samples were incubated on blood agar plates for six hours and identified by the MicroFlex LT (Bruker Daltonics) and Vitek-MS (bioMerieux) systems, followed by AST using the Vitek2 System (bioMerieux). RESULTS: The correct species-level ID rates were 82.3% (209/254) and 78.3% (199/254) for the MicroFlex LT and Vitek-MS platforms, respectively. For the 1,174 microorganism/antimicrobial agent combinations tested, the rapid AST method showed total concordance of 97.8% (1,148/1,174) with the conventional method, with a very major error rate of 0.5%, major error rate of 0.7%, and minor error rate of 1.0%. CONCLUSIONS: Routine implementation of this short-term incubation method could provide ID results on the day of blood culture-positivity detection and one day earlier than the conventional AST method. This simple method will be very useful for rapid ID and AST of bacteria from positive blood culture bottles in routine clinical practice.-
dc.description.statementOfResponsibilityopen-
dc.languageEnglish-
dc.publisherKorean Society for Laboratory Medicine-
dc.relation.isPartOfANNALS OF LABORATORY MEDICINE-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleSame-Day Identification and Antimicrobial Susceptibility Testing of Bacteria in Positive Blood Culture Broths Using Short-Term Incubation on Solid Medium with the MicroFlex LT, Vitek-MS, and Vitek2 Systems-
dc.typeArticle-
dc.contributor.collegeOthers-
dc.contributor.departmentSeverance Hospital-
dc.contributor.googleauthorJihye Ha-
dc.contributor.googleauthorSung Kuk Hong-
dc.contributor.googleauthorGeum Hee Han-
dc.contributor.googleauthorMyungsook Kim-
dc.contributor.googleauthorDongeun Yong-
dc.contributor.googleauthorKyungwon Lee-
dc.identifier.doi10.3343/alm.2018.38.3.235-
dc.contributor.localIdA05454-
dc.contributor.localIdA02423-
dc.contributor.localIdA02649-
dc.contributor.localIdA05529-
dc.relation.journalcodeJ00164-
dc.identifier.eissn2234-3814-
dc.identifier.pmid29401558-
dc.subject.keywordAntimicrobial susceptibility testing-
dc.subject.keywordBlood culture-
dc.subject.keywordMALDI-TOF-MS-
dc.subject.keywordRapid identification-
dc.subject.keywordSepticemia-
dc.contributor.alternativeNameKim, Myungsook-
dc.contributor.alternativeNameYong, Dong Eun-
dc.contributor.alternativeNameLee, Kyung Won-
dc.contributor.alternativeNameHan, Geum Hee-
dc.contributor.affiliatedAuthorKim, Myungsook-
dc.contributor.affiliatedAuthorYong, Dong Eun-
dc.contributor.affiliatedAuthorLee, Kyung Won-
dc.contributor.affiliatedAuthorHan, Geum Hee-
dc.citation.volume38-
dc.citation.number3-
dc.citation.startPage235-
dc.citation.endPage241-
dc.identifier.bibliographicCitationANNALS OF LABORATORY MEDICINE, Vol.38(3) : 235-241, 2018-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers

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