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Exogenous ROS-induced cell sheet transfer based on hematoporphyrin-polyketone film via a one-step process

Authors
 Min-Ah Koo  ;  Mi Hee Lee  ;  Byeong-Ju Kwon  ;  Gyeung Mi Seon  ;  Min Sung Kim  ;  Dohyun Kim  ;  Ki Chang Nam  ;  Jong-Chul Park 
Citation
 Biomaterials, Vol.161 : 47-56, 2018 
Journal Title
 Biomaterials 
ISSN
 0142-9612 
Issue Date
2018
Keywords
Cell sheet detachment ; Exogenous ROS ; Extracellular matrix ; Hp-PK film
Abstract
To date, most of invasive cell sheet harvesting methods have used culture surface property variations, such as wettability, pH, electricity, and magnetism, to induce cell detachment. These methods that rely on surface property changes are effective when cell detachment prior to application is necessary, but of limited use when used for cell sheet transfer to target regions. The study reports a new reactive oxygen species (ROS)-induced strategy based on hematoporphyrin-incorporated polyketone film (Hp-PK film) to transfer cell sheets directly to target areas without an intermediate harvesting process. After green LED (510nm) irradiation, production of exogenous ROS from the Hp-PK films induces cell sheet detachment and transfer. The study suggests that ROS-induced cell detachment property of the Hp-PK film is closely related to conformational changes of extracellular matrix (ECM) proteins. Also, this strategy with the Hp-PK film can be applied by regulating production rate of exogenous ROS in various types of cells, including fibroblasts, mesenchymal stem cells and keratinocytes. In conclusion, ROS-induced method using the Hp-PK film can be used for one-step cell sheet transplantation and has potential in biomedical applications.
Full Text
https://www.sciencedirect.com/science/article/pii/S0142961218300449
DOI
10.1016/j.biomaterials.2018.01.030
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
Yonsei Authors
구민아(Koo, Min-Ah) ORCID logo https://orcid.org/0000-0002-8671-1131
권병주(Kwon, Byeong-Ju) ORCID logo https://orcid.org/0000-0001-9916-0546
박종철(Park, Jong Chul) ORCID logo https://orcid.org/0000-0003-0083-5991
선경미(Seon, Gyeung Mi)
이미희(Lee, Mi Hee) ORCID logo https://orcid.org/0000-0002-9630-7044
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/161931
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