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Neurogenin-2-transduced human neural progenitor cells attenuate neonatal hypoxic-ischemic brain injury.

DC Field Value Language
dc.contributor.author박국인-
dc.contributor.author신정은-
dc.contributor.author황규진-
dc.contributor.author김일선-
dc.date.accessioned2018-07-20T11:58:01Z-
dc.date.available2018-07-20T11:58:01Z-
dc.date.issued2017-
dc.identifier.issn1931-5244-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/161561-
dc.description.abstractNeonatal hypoxic-ischemic (HI) brain injury leads to high mortality and neurodevelopmental disabilities. Multipotent neural progenitor cells (NPCs) with self-renewing capacity have the potential to reduce neuronal loss and improve the compromised environment in the HI brain injury. However, the therapeutic efficacy of neuronal-committed progenitor cells and the underlying mechanisms of recovery are not yet fully understood. Therefore, this study investigated the regenerative ability and action mechanisms of neuronally committed human NPCs (hNPCs) transduced with neurogenin-2 (NEUROG2) in neonatal HI brain injury. NEUROG2- or green fluorescent protein (GFP)-encoding adenoviral vector-transduced hNPCs (NEUROG2- or GFP-NPCs) were transplanted into neonatal mouse brains with HI injury. Grafted NEUROG2-NPCs showed robust dispersion and engraftment, prolonged survival, and neuronal differentiation in HI brain injury. NEUROG2-NPCs significantly improved neurological behaviors, decreased cellular apoptosis, and increased the neurite outgrowth and axonal sprouting in HI brain injury. In contrast, GFP-NPC grafts moderately enhanced axonal extension with limited behavioral recovery. Notably, NEUROG2-NPCs showed increased secretion of multiple factors, such as nerve growth factor, brain-derived neurotrophic factor, neurotrophin-3 (NTF3), fibroblast growth factor 9 (FGF9), ciliary neurotrophic factor (CNTF), and thrombospondins 1 and 2 (THBS 1/2), which promoted SH-SY5Y neuroblastoma cell survival and neurite outgrowth. Thus, we postulate that NEUROG2-expressing human NPCs facilitate functional recovery after neonatal HI brain injury via their ability to secrete multiple factors that enhance neuronal survival and neuroplasticity.-
dc.description.statementOfResponsibilityrestriction-
dc.languageEnglish-
dc.publisherElsevier-
dc.relation.isPartOfTRANSLATIONAL RESEARCH-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHAnimals, Newborn-
dc.subject.MESHBasic Helix-Loop-Helix Transcription Factors/genetics-
dc.subject.MESHBasic Helix-Loop-Helix Transcription Factors/metabolism*-
dc.subject.MESHBrain Injuries/therapy*-
dc.subject.MESHCell Differentiation-
dc.subject.MESHCell Line, Tumor-
dc.subject.MESHCell Transplantation-
dc.subject.MESHGene Expression Regulation-
dc.subject.MESHHumans-
dc.subject.MESHHypoxia-Ischemia, Brain/therapy*-
dc.subject.MESHMice-
dc.subject.MESHMultipotent Stem Cells-
dc.subject.MESHNerve Tissue Proteins/genetics-
dc.subject.MESHNerve Tissue Proteins/metabolism*-
dc.subject.MESHNeural Stem Cells/metabolism*-
dc.subject.MESHNeurons/physiology-
dc.subject.MESHTissue Culture Techniques-
dc.titleNeurogenin-2-transduced human neural progenitor cells attenuate neonatal hypoxic-ischemic brain injury.-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine-
dc.contributor.departmentDept. of Pediatrics-
dc.contributor.googleauthorIl-Shin Lee-
dc.contributor.googleauthorKyo Yeon Koo-
dc.contributor.googleauthorKwangsoo Jung-
dc.contributor.googleauthorMiri Kim-
dc.contributor.googleauthorIl-Sun Kim-
dc.contributor.googleauthorKyujin Hwang-
dc.contributor.googleauthorSeokhwan Yun-
dc.contributor.googleauthorHaejin Lee-
dc.contributor.googleauthorJeong Eun Shin-
dc.contributor.googleauthorKook In Park-
dc.identifier.doi10.1016/j.trsl.2016.12.010-
dc.contributor.localIdA01438-
dc.contributor.localIdA02152-
dc.relation.journalcodeJ03229-
dc.identifier.eissn1878-1810-
dc.identifier.pmid28081931-
dc.identifier.urlhttps://www.sciencedirect.com/science/article/pii/S1931524416304856-
dc.contributor.alternativeNamePark, Kook In-
dc.contributor.alternativeNameShin, Jeong Eun-
dc.contributor.affiliatedAuthorPark, Kook In-
dc.contributor.affiliatedAuthorShin, Jeong Eun-
dc.citation.volume183-
dc.citation.startPage121-
dc.citation.endPage136-
dc.identifier.bibliographicCitationTRANSLATIONAL RESEARCH, Vol.183 : 121-136, 2017-
dc.identifier.rimsid61590-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pediatrics (소아과학교실) > 1. Journal Papers

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