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Mycobacterial cord factor enhances migration of neutrophil-like HL-60 cells by prolonging AKT phosphorylation

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dc.contributor.author김락균-
dc.date.accessioned2018-07-20T11:57:23Z-
dc.date.available2018-07-20T11:57:23Z-
dc.date.issued2017-
dc.identifier.issn0385-5600-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/161547-
dc.description.abstractTrehalose 6,6'-dimycolate (TDM), or cord factor, is a crucial stimulus of immune responses during Mycobacterium tuberculosis infection. Although TDM has immuno-stimulatory properties, including adjuvant activity and the ability to induce granuloma formation, the mechanisms underlying these remain unknown. We hypothesized that TDM stimulates transendothelial migration of neutrophils, which are the first immune cells to infiltrate the tissue upon infection. In this study, it was shown that TDM enhances N-formylmethionyl-leucyl-phenylalanine (fMLP)-induced chemotaxis and transendothelial movement by prolonging AKT phosphorylation in human neutrophils. TDM induced expression of macrophage-inducible C-type lectin, a receptor for TDM, and induced secretion of pro-inflammatory cytokines and chemokines in differentiated HL-60 cells. In 2- and 3-D neutrophil migration assays, TDM-stimulated neutrophils showed increased fMLP-induced chemotaxis and transendothelial migration. Interestingly, following fMLP stimulation of TDM-activated neutrophils, AKT, a crucial kinase for neutrophil polarization and chemotaxis, showed prolonged phosphorylation at serine 473. Taken together, these data suggest that TDM modulates transendothelial migration of neutrophils upon mycobacterial infection through prolonged AKT phosphorylation. AKT may therefore be a promising therapeutic target for enhancing immune responses to mycobacterial infection.-
dc.description.statementOfResponsibilityrestriction-
dc.languageEnglish-
dc.publisherWiley-Blackwell Description: v. ill.-
dc.relation.isPartOfMICROBIOLOGY AND IMMUNOLOGY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleMycobacterial cord factor enhances migration of neutrophil-like HL-60 cells by prolonging AKT phosphorylation-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine-
dc.contributor.departmentDept. of Life Science-
dc.contributor.googleauthorWook‐Bin Lee-
dc.contributor.googleauthorJi‐Jing Yan-
dc.contributor.googleauthorJi‐Seon Kang-
dc.contributor.googleauthorSeok Chung-
dc.contributor.googleauthorLark Kyun Kim-
dc.identifier.doi10.1111/1348-0421.12544-
dc.contributor.localIdA04520-
dc.relation.journalcodeJ02232-
dc.identifier.eissn1348-0421-
dc.identifier.pmid28976590-
dc.identifier.urlhttps://onlinelibrary.wiley.com/doi/full/10.1111/1348-0421.12544-
dc.contributor.alternativeNameKim, Lark Kyun-
dc.contributor.affiliatedAuthorKim, Lark Kyun-
dc.citation.volume61-
dc.citation.number12-
dc.citation.startPage523-
dc.citation.endPage530-
dc.identifier.bibliographicCitationMICROBIOLOGY AND IMMUNOLOGY, Vol.61(12) : 523-530, 2017-
dc.identifier.rimsid61576-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers

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