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Stability of lyophilized pooled sera as quality control materials for tumor marker assays in external quality assessment

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dc.contributor.author김영란-
dc.contributor.author김정호-
dc.contributor.author김현숙-
dc.contributor.author박정용-
dc.date.accessioned2018-07-20T08:30:11Z-
dc.date.available2018-07-20T08:30:11Z-
dc.date.issued2017-
dc.identifier.issn0009-8981-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/161250-
dc.description.abstractBACKGROUND: External quality assessment (EQA) requires stable quality control (QC) materials. We evaluated the stability of QC materials made of lyophilized and liquid pooled sera for the tumor markers α-fetoprotein, carcinoembryonic antigen, carbohydrate antigen 125, and carbohydrate antigen 19-9. METHODS: Specimens of the 4 tumor markers were collected from the sera of patients and stored at -20°C. After sera collection and pooling, liquid or lyophilized samples were stored at -20°C, 5°C, or room temperature. Tumor markers were quantified on days 0, 1, 4, 7, 14, 30, and 90 of storage. Internal QC results were analyzed, and the effects of heat inactivation and sucrose addition were assessed. RESULTS: Heat inactivation lowered tumor marker levels in lyophilized pooled sera, whereas sucrose addition had no effect. The coefficients of variation of the internal QC results were stable, whereas those of lyophilized samples were higher than those of liquid samples. Tumor marker levels were significantly lower in lyophilized samples (p<0.05) and did not significantly differ according to storage temperature. A declining trend over time was observed for all tumor markers. CONCLUSIONS: Lyophilized QC materials are insufficiently stable for use in EQA among clinical laboratories.-
dc.description.statementOfResponsibilityrestriction-
dc.languageEnglish-
dc.publisherElsevier-
dc.relation.isPartOfCLINICA CHIMICA ACTA-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHBiomarkers, Tumor/blood*-
dc.subject.MESHBlood Specimen Collection*-
dc.subject.MESHClinical Laboratory Techniques*-
dc.subject.MESHFreeze Drying-
dc.subject.MESHHumans-
dc.subject.MESHNeoplasms/blood*-
dc.subject.MESHQuality Control*-
dc.titleStability of lyophilized pooled sera as quality control materials for tumor marker assays in external quality assessment-
dc.typeArticle-
dc.contributor.collegeOthers-
dc.contributor.departmentSeverance Hospital-
dc.contributor.googleauthorSunyoung Ahn-
dc.contributor.googleauthorJungyong Park-
dc.contributor.googleauthorYoung Ran Kim-
dc.contributor.googleauthorJeong-Ho Kim-
dc.contributor.googleauthorHyon-Suk Kim-
dc.identifier.doi10.1016/j.cca.2017.05.035-
dc.contributor.localIdA04904-
dc.contributor.localIdA00903-
dc.contributor.localIdA01117-
dc.contributor.localIdA05333-
dc.relation.journalcodeJ00543-
dc.identifier.eissn1873-3492-
dc.identifier.pmid28579142-
dc.identifier.urlhttps://www.sciencedirect.com/science/article/pii/S0009898117302243-
dc.subject.keywordExternal quality assessment-
dc.subject.keywordLyophilization-
dc.subject.keywordQuality control-
dc.subject.keywordStability-
dc.subject.keywordTumor marker-
dc.contributor.alternativeNameKim, Young Lan-
dc.contributor.alternativeNameKim, Jeong Ho-
dc.contributor.alternativeNameKim, Hyon Suk-
dc.contributor.alternativeNamePark, Jungyong-
dc.contributor.affiliatedAuthorKim, Young Lan-
dc.contributor.affiliatedAuthorKim, Jeong Ho-
dc.contributor.affiliatedAuthorKim, Hyon Suk-
dc.contributor.affiliatedAuthorPark, Jungyong-
dc.citation.volume471-
dc.citation.startPage233-
dc.citation.endPage242-
dc.identifier.bibliographicCitationCLINICA CHIMICA ACTA, Vol.471 : 233-242, 2017-
dc.identifier.rimsid61172-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers

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