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11β-Hydroxysteroid Dehydrogenase Type 1 Inhibition Attenuates the Adverse Effects of Glucocorticoids on Dermal Papilla Cells.

DC FieldValueLanguage
dc.contributor.author이상은-
dc.contributor.author이승헌-
dc.date.accessioned2018-07-20T08:21:14Z-
dc.date.available2018-07-20T08:21:14Z-
dc.date.issued2017-
dc.identifier.issn0513-5796-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/161114-
dc.description.abstractPURPOSE: Glucocorticoids, stress-related hormones, inhibit hair growth. Intracellular glucocorticoid availability is regulated by 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1). 11β-HSD1 was recently detected in keratinocytes and fibroblasts. However, the expression of 11β-HSD1 in human hair follicles remains unknown. We aimed to examine 11β-HSD1 expression in human dermal papilla cells (DPCs) and to investigate whether modulation of 11β-HSD1 activity can regulate the negative effects of glucocorticoids on DPCs. MATERIALS AND METHODS: 11β-HSD1 expression in normal human scalp skin was examined by immunohistochemistry. 11β-HSD1 protein was detected in Western blots of human DPCs. Cultured human DPCs were treated with cortisol with or without a selective 11β-HSD1 inhibitor and subsequently stained for Ki-67 antibody. Expression levels of 11β-HSD1, Wnt5a, alkaline phosphatase (ALP), and vascular endothelial growth factor (VEGF) were analyzed by Western blotting. RESULTS: 11β-HSD1 was detected in dermal papilla in human scalp skin by immunohistochemistry. Human DPCs expressed 11β-HSD1 protein in vitro. Furthermore, cortisol stimulated the expression of 11β-HSD1 in DPCs. Glucocorticoids decreased cellular proliferation and the expression of Wnt5a, ALP, and VEGF in DPCs. A specific 11β-HSD1 inhibitor significantly attenuated the anti-proliferative effects of cortisol and reversed the cortisol-induced suppression of Wnt5a, ALP, and VEGF expression in DPCs. CONCLUSION: Our data demonstrated the expression of 11β-HSD1 in human DPCs and revealed that inhibition of 11β-HSD1 activity can partially prevent the negative effect of glucocorticoids on DPCs, suggesting the possible application of 11β-HSD1 inhibitors for stress-related hair loss.-
dc.description.statementOfResponsibilityopen-
dc.languageEnglish-
dc.publisherYonsei University-
dc.relation.isPartOfYONSEI MEDICAL JOURNAL-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESH11-beta-Hydroxysteroid Dehydrogenase Type 1/antagonists & inhibitors*-
dc.subject.MESH11-beta-Hydroxysteroid Dehydrogenase Type 1/genetics-
dc.subject.MESHBlotting, Western-
dc.subject.MESHCell Proliferation/drug effects*-
dc.subject.MESHCells, Cultured-
dc.subject.MESHFibroblasts/metabolism*-
dc.subject.MESHGlucocorticoids/pharmacology*-
dc.subject.MESHHumans-
dc.subject.MESHHydrocortisone-
dc.subject.MESHKeratinocytes/metabolism*-
dc.subject.MESHSignal Transduction-
dc.subject.MESHVascular Endothelial Growth Factor A-
dc.title11β-Hydroxysteroid Dehydrogenase Type 1 Inhibition Attenuates the Adverse Effects of Glucocorticoids on Dermal Papilla Cells.-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine-
dc.contributor.departmentDept. of Dermatology-
dc.contributor.googleauthorSang Eun Lee-
dc.contributor.googleauthorEun Young Lee-
dc.contributor.googleauthorSang Jin Kang-
dc.contributor.googleauthorSeung Hun Lee-
dc.identifier.doi10.3349/ymj.2017.58.6.1204-
dc.contributor.localIdA02826-
dc.contributor.localIdA02931-
dc.relation.journalcodeJ02813-
dc.identifier.eissn1976-2437-
dc.identifier.pmid29047245-
dc.subject.keyword11β-hydroxysteroid dehydrogenase type 1-
dc.subject.keyworddermal papilla cells-
dc.subject.keywordglucocorticoids-
dc.contributor.alternativeNameLee, Sang Eun-
dc.contributor.alternativeNameLee, Seung Hun-
dc.contributor.affiliatedAuthorLee, Sang Eun-
dc.contributor.affiliatedAuthorLee, Seung Hun-
dc.citation.volume58-
dc.citation.number6-
dc.citation.startPage1204-
dc.citation.endPage1210-
dc.identifier.bibliographicCitationYONSEI MEDICAL JOURNAL, Vol.58(6) : 1204-1210, 2017-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실) > 1. Journal Papers

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