Cited 7 times in
Montelukast improves the changes of cytoskeletal and adaptor proteins of human podocytes by interleukin-13
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 신재일 | - |
dc.date.accessioned | 2018-07-20T08:14:24Z | - |
dc.date.available | 2018-07-20T08:14:24Z | - |
dc.date.issued | 2017 | - |
dc.identifier.issn | 1023-3830 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/161004 | - |
dc.description.abstract | OBJECTIVE AND DESIGN: Interleukin-13 (IL-13) has recently been reported to be a potential cytokine in the pathogenesis of minimal-change nephrotic syndrome (MCNS). However, the mechanistic insights associated with podocyte dysfunction mediated by IL-13-induced changes in various slit diaphragm (SD) and cytoskeletal molecules have not yet been shown in cultured human podocytes in vitro. MATERIALS: Human conditionally immortalized podocytes were used. TREATMENT: Podocytes were incubated with various concentrations of IL-13 during the indicated time periods (6, 12, and 24 h) and montelukast was administered with the dose of 0.1 μg. RESULTS: Treatment of IL-13 resulted in a progressive decrease in distinct processes or projections of the human podocytes and high dose of IL-13 increased podocyte permeability in vitro at 6 h. IL-13 had a substantial impact on the redistribution and rearrangement of zonula occludens (ZO)-1, synaptopodin, α-actinin, CD2-associated protein (CD2AP) in podocytes and disrupted the cytoskeletal connections in a concentration-dependent manner on confocal microscopy. IL-13 also down-modulated ZO-1, synaptopodin, α-actinin, CD2AP, and p130Cas at protein levels and upregulated β-catenin and B7-1 in podocytes. Furthermore, we demonstrated that down-modulated changes in various SD and cytoskeletal structures of human podocytes induced by IL-13 was significantly restored after treatment with montelukast with upregulation of B7-1. CONCLUSION: Our results suggest that targeting IL-13 may be one of the important cytokines in the pathogenesis of MCNS and targeting IL-13 could be one of the potential therapeutic strategies in MCNS. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Birkhäuser | - |
dc.relation.isPartOf | INFLAMMATION RESEARCH | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Acetates/pharmacology* | - |
dc.subject.MESH | Actinin/metabolism | - |
dc.subject.MESH | Adaptor Proteins, Signal Transducing/metabolism | - |
dc.subject.MESH | B7-1 Antigen/metabolism | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Crk-Associated Substrate Protein/metabolism | - |
dc.subject.MESH | Cytoskeletal Proteins/metabolism | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Interleukin-13/pharmacology* | - |
dc.subject.MESH | Leukotriene Antagonists/pharmacology* | - |
dc.subject.MESH | Microfilament Proteins/metabolism | - |
dc.subject.MESH | Podocytes/drug effects* | - |
dc.subject.MESH | Podocytes/metabolism | - |
dc.subject.MESH | Podocytes/ultrastructure | - |
dc.subject.MESH | Quinolines/pharmacology* Zonula Occludens-1 Protein/metabolism | - |
dc.subject.MESH | beta Catenin/metabolism | - |
dc.title | Montelukast improves the changes of cytoskeletal and adaptor proteins of human podocytes by interleukin-13 | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine | - |
dc.contributor.department | Dept. of Pediatrics | - |
dc.contributor.googleauthor | Tae-Sun Ha | - |
dc.contributor.googleauthor | Ja Ae Nam | - |
dc.contributor.googleauthor | Su-Bin Seong | - |
dc.contributor.googleauthor | Moin A. Saleem | - |
dc.contributor.googleauthor | Se Jin Park | - |
dc.contributor.googleauthor | Jae Il Shin | - |
dc.identifier.doi | 10.1007/s00011-017-1058-y | - |
dc.contributor.localId | A02142 | - |
dc.relation.journalcode | J01059 | - |
dc.identifier.eissn | 1420-908X | - |
dc.identifier.pmid | 28608180 | - |
dc.identifier.url | https://link.springer.com/article/10.1007%2Fs00011-017-1058-y | - |
dc.subject.keyword | B7-1 | - |
dc.subject.keyword | Cytoskeletal molecules | - |
dc.subject.keyword | Interleukin-13 | - |
dc.subject.keyword | Leukotriene receptor antagonists | - |
dc.subject.keyword | Podocytes | - |
dc.subject.keyword | Slit diaphragm | - |
dc.contributor.alternativeName | Shin, Jae Il | - |
dc.contributor.affiliatedAuthor | Shin, Jae Il | - |
dc.citation.volume | 66 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 793 | - |
dc.citation.endPage | 802 | - |
dc.identifier.bibliographicCitation | INFLAMMATION RESEARCH, Vol.66(9) : 793-802, 2017 | - |
dc.identifier.rimsid | 60895 | - |
dc.type.rims | ART | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.