0 134

Cited 3 times in

Validation of ALK/ROS1 Dual Break Apart FISH Probe probe in non-small-cell lung cancer

DC FieldValueLanguage
dc.contributor.author김혜련-
dc.contributor.author심효섭-
dc.contributor.author조병철-
dc.contributor.author차윤진-
dc.date.accessioned2018-07-20T08:06:54Z-
dc.date.available2018-07-20T08:06:54Z-
dc.date.issued2017-
dc.identifier.issn0169-5002-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/160848-
dc.description.abstractBACKGROUND: ALK and ROS1 gene rearrangements are distinct molecular subsets of non-small-cell lung cancer (NSCLC), and they are strong predictive biomarkers of response to ALK/ROS1 inhibitors, such as crizotinib. Thus, it is clinically important to develop an effective screening strategy to detect patients who will benefit from such treatment. In this study, we aimed to validate analytical performance of Vysis ALK/ROS1 Dual Break Apart Probe Kit (RUO) in NSCLC. METHODS: Study population composed of three patient cohorts with histologically confirmed lung adenocarcinoma (patients with ALK rearrangement, patients with ROS1 rearrangement and patients with wild-type ALK and ROS1). Specimens consisted of 12 ALK-positive, 8 ROS1-positive and 21 ALK/ROS1-wild type formalin-fixed paraffin-embedded samples obtained from surgical resection or excisional biopsy. ALK rearrangement was previously assessed by Vysis ALK Break Apart FISH Probe Kit (Abbott Molecular, Abbot Park, IL, USA) and ROS1 rearrangement was previously assessed by ZytoLight® SPEC ROS1 Break Apart Probe (ZytoVision, GmbH). All specimens were re-evaluated by Vysis ALK/ROS1 Dual Break Apart Probe Kit. FISH images were scanned on BioView AllegroPlus system and interpreted via BioView SoloWeb remotely. RESULTS: For a total of 41 patient samples, the concordance of the results by Vysis ALK/ROS1 Dual Break Apart Probe Kit was evaluated and compared to the known ALK and ROS1 rearrangement status of the specimen. Of the 12 ALK-positive cases, hybridization with Vysis ALK/ROS1 Dual Break Apart Probe Kit was successful in 10 cases (success rate 10/12, 83%) and of these 10 cases, all showed ALK rearrangement (100% concordance with the results of Vysis ALK Break Apart FISH Probe Kit). Two of the ALK+ cases were excluded due to weak ROS1 signals that could not be enumerated. Of the 8 ROS1-positive cases, 6 cases were successfully evaluated using Vysis ALK/ROS1 Dual Break Apart Probe Kit. The success rate was 75% (6/8), and of these 6 cases, all showed ROS1 rearrangement, giving a 100% concordance with ZytoLight® SPEC ROS1 Break Apart Probe. Two of the cases were excluded due to weak ROS1 gold signal or high background. In the cohort of 21 wild-type cases, the success rate using Vysis ALK/ROS1 Dual Break Apart FISH Probe Kit was 85% (18/21) and the concordance with ALK and ROS1 probe kit was 100% (18/18). CONCLUSION: Vysis ALK/ROS1 Dual Break Apart Probe Kit (RUO) can detect ALK and ROS1 rearrangement simultaneously in NSCLC.-
dc.description.statementOfResponsibilityrestriction-
dc.languageEnglish-
dc.publisherElsevier Scientific Publishers-
dc.relation.isPartOfLUNG CANCER-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAdult-
dc.subject.MESHAged-
dc.subject.MESHBiomarkers, Tumor-
dc.subject.MESHCarcinoma, Non-Small-Cell Lung/diagnosis-
dc.subject.MESHCarcinoma, Non-Small-Cell Lung/genetics-
dc.subject.MESHDNA Probes-
dc.subject.MESHFemale-
dc.subject.MESHGene Rearrangement-
dc.subject.MESHGenotype-
dc.subject.MESHHumans-
dc.subject.MESHIn Situ Hybridization, Fluorescence*/methods-
dc.subject.MESHLung Neoplasms/diagnosis-
dc.subject.MESHLung Neoplasms/genetics-
dc.subject.MESHMale-
dc.subject.MESHMiddle Aged-
dc.subject.MESHMutation-
dc.subject.MESHNeoplasm Staging-
dc.subject.MESHOncogene Proteins, Fusion/genetics-
dc.subject.MESHProtein-Tyrosine Kinases/genetics-
dc.subject.MESHProto-Oncogene Proteins/genetics-
dc.subject.MESHReagent Kits, Diagnostic-
dc.subject.MESHReceptor Protein-Tyrosine Kinases/genetics-
dc.subject.MESHReproducibility of Results-
dc.titleValidation of ALK/ROS1 Dual Break Apart FISH Probe probe in non-small-cell lung cancer-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine-
dc.contributor.departmentDept. of Internal Medicine-
dc.contributor.googleauthorSun Min Lim-
dc.contributor.googleauthorHyun Chang-
dc.contributor.googleauthorYoon Jin Cha-
dc.contributor.googleauthorShile Liang-
dc.contributor.googleauthorYan Chin Tai-
dc.contributor.googleauthorGu Li-
dc.contributor.googleauthorEkaterina Pestova-
dc.contributor.googleauthorFrank Policht-
dc.contributor.googleauthorThomas Perez-
dc.contributor.googleauthorRoss A. Soo-
dc.contributor.googleauthorWon Young Park-
dc.contributor.googleauthorHye Ryun Kim-
dc.contributor.googleauthorHyo Sup Shim-
dc.contributor.googleauthorByoung Chul Cho-
dc.identifier.doi10.1016/j.lungcan.2017.07.016-
dc.contributor.localIdA01166-
dc.contributor.localIdA02219-
dc.contributor.localIdA03822-
dc.contributor.localIdA04001-
dc.relation.journalcodeJ02174-
dc.identifier.eissn1872-8332-
dc.identifier.pmid28838404-
dc.identifier.urlhttps://www.sciencedirect.com/science/article/pii/S0169500217303951-
dc.subject.keywordALK-
dc.subject.keywordFluorescent in situ hybridization-
dc.subject.keywordNon-small-cell lung cancer-
dc.subject.keywordROS1-
dc.contributor.alternativeNameKim, Hye Ryun-
dc.contributor.alternativeNameShim, Hyo Sup-
dc.contributor.alternativeNameCho, Byoung Chul-
dc.contributor.alternativeNameCha, Yoon Jin-
dc.contributor.affiliatedAuthorKim, Hye Ryun-
dc.contributor.affiliatedAuthorShim, Hyo Sup-
dc.contributor.affiliatedAuthorCho, Byoung Chul-
dc.contributor.affiliatedAuthorCha, Yoon Jin-
dc.citation.volume111-
dc.citation.startPage79-
dc.citation.endPage83-
dc.identifier.bibliographicCitationLUNG CANCER, Vol.111 : 79-83, 2017-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.