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Distal-less homeobox 5 is a master regulator of the osteogenesis of human mesenchymal stem cells

DC FieldValueLanguage
dc.contributor.author김현옥-
dc.contributor.author허준석-
dc.date.accessioned2018-07-20T08:06:17Z-
dc.date.available2018-07-20T08:06:17Z-
dc.date.issued2017-
dc.identifier.issn1107-3756-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/160841-
dc.description.abstractMesenchymal stem cells (MSCs) differentiate into multiple lineages and are a promising source of cells for clinical use. Previously, we found that the gene distal‑less homeobox 5 (DLX5) is specifically expressed in MSCs with osteogenic potential. Understanding the mechanism of osteogenesis is necessary for successful bone regeneration using MSCs. The aim of this study was to examine the function of the DLX5 gene in MSCs during osteogenesis (bone development). We analyzed the possible association between DLX5 expression and osteogenesis-, chondrogenesis- and adipogenesis-related gene expression in different cells isolated from bone marrow and cord blood. Differentiation capacity was assessed by observing morphological changes, monitoring gene expression patterns, and staining with Von Kossa, safranin O, and Oil Red O. Suppression of DLX5 expression by means of a small interfering RNA (siRNA) downregulated osteogenic markers and reduced the signs of calcium mineralization. Tanshinone IIA is a known small molecule activator of bone morphogenetic protein (BMP) signaling. Here, we report that induction of DLX5 by tanshinone IIA in MSCs enhanced osteogenic differentiation. In addition, we showed that tanshinone IIA (as a mediator of BMP2 signaling) activates runt-related transcription factor 2 (RUNX2) in MSCs and initiates calcium mineralization during osteogenesis. Taken together, these findings indicate that, in MSCs, DLX5 is a master regulator of osteogenesis. Furthermore, tanshinone IIA may be valuable for stem cell-based therapies of certain bone diseases.-
dc.description.statementOfResponsibilityopen-
dc.languageEnglish-
dc.publisherD.A. Spandidos-
dc.relation.isPartOfInternational Journal of Molecular Medicine-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHBone Morphogenetic Protein 2/metabolism-
dc.subject.MESHCell Differentiation/genetics*-
dc.subject.MESHCell Survival/genetics-
dc.subject.MESHCells, Cultured-
dc.subject.MESHDiterpenes, Abietane/pharmacology-
dc.subject.MESHFetal Blood/cytology-
dc.subject.MESHGene Expression Regulation, Developmental*-
dc.subject.MESHHomeodomain Proteins/genetics*-
dc.subject.MESHHumans-
dc.subject.MESHMesenchymal Stromal Cells/cytology*-
dc.subject.MESHMesenchymal Stromal Cells/metabolism*-
dc.subject.MESHOsteogenesis/genetics*-
dc.subject.MESHRNA Interference-
dc.subject.MESHRNA, Small Interfering/genetics-
dc.subject.MESHSignal Transduction/drug effects-
dc.subject.MESHTranscription Factors/genetics*-
dc.titleDistal-less homeobox 5 is a master regulator of the osteogenesis of human mesenchymal stem cells-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine-
dc.contributor.departmentDept. of Laboratory Medicine-
dc.contributor.googleauthorJUNE SEOK HEO-
dc.contributor.googleauthorSEUNG GWAN LEE-
dc.contributor.googleauthorHYUN OK KIM-
dc.identifier.doi10.3892/ijmm.2017.3142-
dc.contributor.localIdA01122-
dc.contributor.localIdA04704-
dc.relation.journalcodeJ01132-
dc.identifier.eissn1791-244X-
dc.identifier.pmid28949384-
dc.contributor.alternativeNameKim, Hyun Ok-
dc.contributor.alternativeNameHeo, June Seok-
dc.contributor.affiliatedAuthorKim, Hyun Ok-
dc.contributor.affiliatedAuthorHeo, June Seok-
dc.citation.volume40-
dc.citation.number5-
dc.citation.startPage1486-
dc.citation.endPage1494-
dc.identifier.bibliographicCitationInternational Journal of Molecular Medicine, Vol.40(5) : 1486-1494, 2017-
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실)

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