92 125

Cited 3 times in

Hypothermia Identifies Dynamin as a Potential Therapeutic Target in Experimental Stroke.

Authors
 Jong Youl Kim  ;  Nuri Kim  ;  Jong Eun Lee  ;  Midori A. Yenari 
Citation
 THERAPEUTIC HYPOTHERMIA AND TEMPERATURE MANAGEMENT, Vol.7(3) : 171-177, 2017 
Journal Title
 THERAPEUTIC HYPOTHERMIA AND TEMPERATURE MANAGEMENT 
ISSN
 2153-7658 
Issue Date
2017
MeSH
Animals ; Apoptosis ; Brain/metabolism ; Brain/pathology ; Brain Ischemia/metabolism ; Disease Models, Animal ; Dynamins/metabolism* ; Fas Ligand Protein/metabolism ; Hypothermia, Induced* ; Male ; Mice ; Mice, Inbred C57BL ; Random Allocation ; Stroke/metabolism*
Keywords
Fas ; apoptosis ; dynamin ; hypothermia ; ischemic stroke
Abstract
Apoptosis is a cell death pathway that is activated in ischemic stroke. The interaction between Fas and its ligand (FasL) initiates a complex pattern of intracellular events involving the recruitment of specific adaptor proteins and the development of apoptosis. We recently reported that dynamin is increased after experimental stroke, and its inhibition improves neurological outcome. Dynamin has been shown to transport Fas from the endoplasmic reticulum to the cell surface where it can be bound by its ligand, FasL. Hypothermia has been shown to improve outcome in numerous stroke models, and this protection is associated with reduced apoptosis and Fas expression. To explore the contribution of dynamin to hypothermic neuroprotection, we subjected mice to distal middle cerebral artery occlusion (dMCAO) and applied one of two cooling paradigms: one where cooling began at the onset of dMCAO (early hypothermia) and another where cooling began 1 hour later (delayed hypothermia), compared with normothermia (Norm). Both cooling paradigms reduced numbers of apoptotic cells, as well as Fas and dynamin compared with Norm. Fas and dynamin were co-expressed in neurons. Neuronal cultures were exposed to oxygen glucose deprivation. Hypothermia decreased dynamin as well as surface expression of Fas, and this correlated to reduced cell death. The results of this study suggest that dynamin may participate in the Fas-mediated apoptotic pathway, and its reduction may be linked to hypothermic neuroprotection.
Files in This Item:
T201703476.pdf Download
DOI
10.1089/ther.2017.0005
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers
Yonsei Authors
Kim, Jong Youl(김종열) ORCID logo https://orcid.org/0000-0002-8340-2894
Lee, Jong Eun(이종은) ORCID logo https://orcid.org/0000-0001-6203-7413
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/160836
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse