0 57

Cited 1 times in

Frequent oncogenic BRAF V600E mutation in odontogenic keratocyst

Authors
 Yong Hoon Cha  ;  Eunae Sandra Cho  ;  Hee Eun Kang  ;  Jaemin Ko  ;  Woong Nam  ;  Hyung Jun Kim  ;  Nam Hee Kim  ;  Hyun Sil Kim  ;  In-Ho Cha  ;  Jong In Yook 
Citation
 Oral Oncology, Vol.74 : 62-67, 2017 
Journal Title
 Oral Oncology 
ISSN
 1368-8375 
Issue Date
2017
Keywords
BRAF V600E ; Keratocystic odontogenic tumor ; Mutation ; Odontogenic keratocyst
Abstract
OBJECTIVES: Odontogenic keratocyst (OKC), also known as keratocystic odontogenic tumor (KCOT), has clinical significance due to its high incidence as well as high recurrence rate after surgical enucleation. Current clinical management for OCK is entirely dependent on surgical approach. While various genetic alterations, such as PTCH1 mutation and loss of heterozygosity in tumor suppressor genes, have been reported, the molecular background of OKC is not well-understood. Although recent identification of BRAF V600E mutation and subsequent activation of mitogen-activated protein kinase (MAPK) pathway in ameloblastoma and odontogenic tumors provide additional options with targeted therapeutics, the molecular background of OKC is not well understood. MATERIALS AND METHODS: In this study, we examined BRAF V600E mutation from paraffin embedded OKC samples by tumor cell enriched microdissection and TA cloning of amplified DNA. We further examined the relationship between BRAF V600E mutation and clinical parameters. RESULTS: We found frequent BRAF V600E mutation in OKC (24 of 38 samples, 63.2%). However, BRAF V600E mutational status is not related with clinical indexes such as size, location, and recurrence. In orthokeratinized odontogenic cyst, there is one case of BRAF 600E mutation from 11 samples (9.1%). CONCLUSION: These results indicate that BRAF V600E mutation occurs in OKCs at a high rate and plays an important role in the pathogenesis of OKCs.
Full Text
https://www.sciencedirect.com/science/article/pii/S1368837517302944
DOI
10.1016/j.oraloncology.2017.09.016
Appears in Collections:
1. Journal Papers (연구논문) > 2. College of Dentistry (치과대학) > Dept. of Orthodontics (교정과학교실)
1. Journal Papers (연구논문) > 5. Research Institutes (연구소) > Oral Cancer Research Institute (구강종양연구소)
1. Journal Papers (연구논문) > 2. College of Dentistry (치과대학) > Dept. of Oral Pathology (구강병리학교실)
1. Journal Papers (연구논문) > 2. College of Dentistry (치과대학) > Dept. of Oral and Maxillofacial Surgery (구강악안면외과학교실)
Yonsei Authors
고재민(Ko, Jaemin)
김남희(Kim, Nam Hee) ORCID logo https://orcid.org/0000-0002-3087-5276
김현실(Kim, Hyun Sil) ORCID logo https://orcid.org/0000-0003-3614-1764
김형준(Kim, Hyung Jun) ORCID logo https://orcid.org/0000-0001-8247-4004
남웅(Nam, Woong) ORCID logo https://orcid.org/0000-0003-0146-3624
육종인(Yook, Jong In) ORCID logo https://orcid.org/0000-0002-7318-6112
차용훈(Cha, Yong Hoon)
차인호(Cha, In Ho) ORCID logo https://orcid.org/0000-0001-8259-2190
Export
RIS (EndNote)
XLS (Excel)
XML
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/160830
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse