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Homogeneity evaluation of mesenchymal stem cells based on electrotaxis analysis

Authors
 Min Sung Kim  ;  Mi Hee Lee  ;  Byeong-Ju Kwon  ;  Dohyun Kim  ;  Min-Ah Koo  ;  Gyeung Mi Seon  ;  Jong-Chul Park 
Citation
 Scientific Reports, Vol.7(1) : 9582, 2017 
Journal Title
 Scientific Reports 
Issue Date
2017
Abstract
Stem cell therapy that can restore function to damaged tissue, avoid host rejection and reduce inflammation throughout body without use of immunosuppressive drugs. The established methods were used to identify and to isolate specific stem cell markers by FACS or by immunomagnetic cell separation. The procedures for distinguishing population of stem cells took a time and needed many preparations. Here we suggest an electrotaxis analysis as a new method to evaluate the homogeneity of mesenchymal stem cells which can observe the stem cell population in culture condition and wide use to various types of stem cells. Human mesenchymal stem cell, adipose derived stem cell, tonsil derived stem cell and osteogenic differentiated cells migrated toward anode but the migration speed of differentiated cells was significantly decreased versus that of stem cells. In mixture of stem cells and differentiated cells condition, we identified that the ratio of stem cell versus differentiated cell was matched with the homogeneity evaluation data of stem cells based on electrotaxis analysis. As a result, our evaluation tool has the possibility of the wide use to stem cell homogeneity evaluation and might be used as the stem cell quality control during stem cell culture without any additional antibodies.
Files in This Item:
T201703266.pdf Download
DOI
10.1038/s41598-017-09543-0
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
Yonsei Authors
구민아(Koo, Min-Ah) ORCID logo https://orcid.org/0000-0002-8671-1131
권병주(Kwon, Byeong-Ju) ORCID logo https://orcid.org/0000-0001-9916-0546
박종철(Park, Jong Chul) ORCID logo https://orcid.org/0000-0003-0083-5991
선경미(Seon, Gyeung Mi) ORCID logo https://orcid.org/0000-0002-0766-1106
이미희(Lee, Mi Hee) ORCID logo https://orcid.org/0000-0002-9630-7044
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/160767
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