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Semliki Forest Virus 감염은 뉴우런의 탈수초를 유발한다

Other Titles
 Infection of Semliki Forest Virus Induces Demyelination of Neuron 
Authors
 김현주  ;  사영희  ;  홍성갑 
Citation
 Journal of the Korean Institute of Maritime Information & Communication Sciences (한국정보통신학회논문지), Vol.21(6) : 1212-1217, 2017 
Journal Title
 Journal of the Korean Institute of Maritime Information & Communication Sciences (한국정보통신학회논문지) 
ISSN
 2234-4772 
Issue Date
2017
Keywords
demyelination ; myelination ; peripheral myelin protein 22 ; Semliki forest virus
Abstract
We constructed a population of myelinated cells with co-culture of neuronal cells and Schwann cells from DRG. Schwann cells and neuronal cells were isolated from dorsal root ganglion (DRG) in embryos of rat in vitro respectively. The cultured Schwann cells and cultured neuronal cells, respectively were co-cultured in a same plate. This procedure contains following four steps: first step of suspension of the embryonic dorsal root ganglion cells, second step of addition of anti-mitoticcocktail, third step of purification of dorsal root cells, and fourth step of addition of Schwann cells to dorsal root ganglion cells. These cells were performed accomplishment of myelination. This myelinated co-culture system was infected by Semliki forest virus and then induced demyelination processing in this myelinated co-culture. We identified myelination and demyelination processing using antibody of peripheral myelin protein 22 (PMP 22) meaning presence of myelinated neuron.
Files in This Item:
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DOI
10.6109/jkiice.2017.21.6.1212
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Anesthesiology and Pain Medicine (마취통증의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Kim, Hyun Joo(김현주) ORCID logo https://orcid.org/0000-0003-1963-8955
Hong, Seong Karp(홍성갑)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/160747
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