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Dexmedetomidine Inhibits Phenylephrine-induced Contractions via Alpha-1 Adrenoceptor Blockade and Nitric Oxide Release in Isolated Rat Aortae
DC Field | Value | Language |
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dc.contributor.author | 변효진 | - |
dc.date.accessioned | 2018-07-20T07:56:16Z | - |
dc.date.available | 2018-07-20T07:56:16Z | - |
dc.date.issued | 2017 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/160686 | - |
dc.description.abstract | The goal of this in vitro study was to examine the effect of the alpha-2 adrenoceptor agonist dexmedetomidine on phenylephrine (alpha-1 adrenoceptor agonist)-induced contraction in isolated rat aortae and to elucidate the associated cellular mechanisms, with a particular focus on alpha-1 adrenoceptor antagonism. Dexmedetomidine dose-response curves were generated in isolated endothelium-intact and endothelium-denuded rat aortae precontracted with phenylephrine or 5-hydroxytryptamine. Endothelium-denuded aortic rings were pretreated with either dexmedetomidine or the reversible alpha-1 adrenoceptor antagonist phentolamine, followed by post-treatment with the irreversible alpha-1 adrenoceptor blocker phenoxybenzamine. Control rings were treated with phenoxybenzamine alone. All rings were repeatedly washed with Krebs solution to remove all pretreatment drugs, including phenoxybenzamine, phentolamine and dexmedetomidine. Phenylephrine dose-response curves were then generated. The effect of rauwolscine on the dexmedetomidine-mediated change in phenylephrine-induced endothelial nitric oxide synthase (eNOS) phosphorylation in human umbilical vein endothelial cells was examined using western blotting. The magnitude of the dexmedetomidine-mediated inhibition of phenylephrine-induced contraction was higher in endothelium-intact aortae than in endothelium-denuded aortae or endothelium-intact aortae treated with Nω-nitro-L-arginine methyl ester. However, dexmedetomidine did not significantly alter 5-hydroxytryptamine-induced contraction. In further experiments, prazosin attenuated dexmedetomidine-induced contraction. Additionally, pretreatment with either dexmedetomidine plus phenoxybenzamine or phentolamine plus phenoxybenzamine produced greater phenylephrine-induced contraction than phenoxybenzamine alone, suggesting that dexmedetomidine protects aortae from the alpha-1 adrenoceptor blockade induced by phenoxybenzamine. Rauwolscine attenuated the dexmedetomidine-mediated enhancement of phenylephrine-induced eNOS phosphorylation. Taken together, these results suggest that dexmedetomidine attenuates phenylephrine-induced contractions via alpha-1 adrenoceptor blockade and endothelial nitric oxide release in the isolated rat aorta. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Ivyspring International Publisher | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF MEDICAL SCIENCES | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Adrenergic Agonists/pharmacology | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Aorta/drug effects* | - |
dc.subject.MESH | Aorta/metabolism* | - |
dc.subject.MESH | Dexmedetomidine/pharmacology* | - |
dc.subject.MESH | In Vitro Techniques | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Muscle Contraction/drug effects | - |
dc.subject.MESH | Nitric Oxide/metabolism* | - |
dc.subject.MESH | Phenoxybenzamine/pharmacology | - |
dc.subject.MESH | Phenylephrine/pharmacology* | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Receptors, Adrenergic/metabolism* | - |
dc.subject.MESH | Serotonin/pharmacology | - |
dc.title | Dexmedetomidine Inhibits Phenylephrine-induced Contractions via Alpha-1 Adrenoceptor Blockade and Nitric Oxide Release in Isolated Rat Aortae | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine | - |
dc.contributor.department | Dept. of Anesthesiology and Pain Medicine | - |
dc.contributor.googleauthor | Hyo-Jin Byon | - |
dc.contributor.googleauthor | Seong-Ho Ok | - |
dc.contributor.googleauthor | Soo Hee Lee | - |
dc.contributor.googleauthor | Sebin Kang | - |
dc.contributor.googleauthor | Youngil Cho | - |
dc.contributor.googleauthor | Jeong Yeol Han | - |
dc.contributor.googleauthor | Ju-Tae Sohn | - |
dc.identifier.doi | 10.7150/ijms.17456 | - |
dc.contributor.localId | A01863 | - |
dc.relation.journalcode | J02917 | - |
dc.identifier.eissn | 1449-1907 | - |
dc.identifier.pmid | 28260990 | - |
dc.subject.keyword | alpha-1 adrenoceptor | - |
dc.subject.keyword | alpha-2 adrenoceptor agonist | - |
dc.subject.keyword | aorta | - |
dc.subject.keyword | contraction | - |
dc.subject.keyword | dexmedetomidine | - |
dc.subject.keyword | nitric oxide | - |
dc.subject.keyword | phenoxybenzamine | - |
dc.subject.keyword | phentolamine | - |
dc.subject.keyword | phenylephrine | - |
dc.contributor.alternativeName | Byon, Hyo Jin | - |
dc.contributor.affiliatedAuthor | Byon, Hyo Jin | - |
dc.citation.volume | 14 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 143 | - |
dc.citation.endPage | 149 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF MEDICAL SCIENCES, Vol.14(2) : 143-149, 2017 | - |
dc.identifier.rimsid | 42495 | - |
dc.type.rims | ART | - |
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