0 325

Cited 2 times in

Competent antigen-presenting cells are generated from the long-term culture of splenocytes with granulocyte-macrophage colony-stimulating factor

DC Field Value Language
dc.contributor.author나혜영-
dc.contributor.author박채규-
dc.date.accessioned2018-07-20T07:53:53Z-
dc.date.available2018-07-20T07:53:53Z-
dc.date.issued2017-
dc.identifier.issn0165-2478-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/160642-
dc.description.abstractDendritic cells (DCs) are routinely produced from the culture of mouse bone marrow (BM) with granulocyte-macrophage colony-stimulating factor (GM-CSF) within a period of 10days. Although splenic extramedullary myelopoiesis was suggested to occur under the influence of GM-CSF, the hematopoietic outcome of splenic culture with GM-CSF has not been scrutinized. We have cultured mouse splenocytes with GM-CSF for an extended period of time, where we discovered that the CD11b⁺CD11c⁺ cells began to proliferate prominently after 10days and their number increased until the 4th week of the culture. In parallel experiments, FMS-like tyrosine kinase 3 (FLT3) and its ligand, FLT3L, were not found to influence the culture of splenocytes. Like DCs in the culture of BM with GM-CSF, a distinct population of CD11b⁺CD11c⁺MHC IIhi cells was readily identified as DCs in the long-term culture of splenocytes. After being isolated and plated overnight the CD11b⁺CD11c⁺MHC IIhi cells exhibited non-adherent dendritic morphology, while the other CD11b⁺CD11c⁺ cells became adherent. Besides, these CD11b⁺CD11c⁺MHC IIhi cells possessed relatively weak endocytic and phagocytic abilities but displayed strong antigen-presenting capacities, revealing DC-like characteristics; in contrast, the other CD11b⁺CD11c⁺ cells showed strong endocytosis and phagocytosis of antigens but were poor at antigen presentation, indicating macrophage-like traits. Therefore, we demonstrated that phenotypically as well as functionally genuine DCs are generated in the long-term culture of splenocytes with GM-CSF.-
dc.description.statementOfResponsibilityrestriction-
dc.languageEnglish-
dc.publisherElsevier/North-Holland Biomedical Press-
dc.relation.isPartOfIMMUNOLOGY LETTERS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHAntigen Presentation/immunology-
dc.subject.MESHAntigen-Presenting Cells/cytology-
dc.subject.MESHAntigen-Presenting Cells/drug effects*-
dc.subject.MESHAntigen-Presenting Cells/immunology*-
dc.subject.MESHAntigen-Presenting Cells/metabolism-
dc.subject.MESHBiomarkers-
dc.subject.MESHBone Marrow-
dc.subject.MESHCells-
dc.subject.MESHCell Culture Techniques-
dc.subject.MESHCells, Cultured-
dc.subject.MESHDendritic Cells/immunology-
dc.subject.MESHDendritic Cells/metabolism-
dc.subject.MESHGranulocyte-Macrophage Colony-Stimulating Factor/pharmacology*-
dc.subject.MESHHistocompatibility Antigens Class II/immunology-
dc.subject.MESHImmunophenotyping-
dc.subject.MESHMembrane Proteins/metabolism-
dc.subject.MESHMice-
dc.subject.MESHMice, Transgenic-
dc.subject.MESHSignal Transduction-
dc.subject.MESHSpleen/cytology*-
dc.subject.MESHSpleen/immunology*-
dc.subject.MESHSpleen/metabolism-
dc.subject.MESHT-Lymphocyte Subsets/immunology-
dc.subject.MESHT-Lymphocyte Subsets/metabolism-
dc.subject.MESHfms-Like Tyrosine Kinase 3/metabolism-
dc.titleCompetent antigen-presenting cells are generated from the long-term culture of splenocytes with granulocyte-macrophage colony-stimulating factor-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine-
dc.contributor.departmentDept. of Life Science-
dc.contributor.googleauthorSeul Hye Ryu-
dc.contributor.googleauthorHye Young Na-
dc.contributor.googleauthorMoah Sohn-
dc.contributor.googleauthorWanho Choi-
dc.contributor.googleauthorHyunju In-
dc.contributor.googleauthorHyun Soo Shin-
dc.contributor.googleauthorJae-Hoon Choi-
dc.contributor.googleauthorChae Gyu Park-
dc.identifier.doi10.1016/j.imlet.2017.06.010-
dc.contributor.localIdA04556-
dc.contributor.localIdA01718-
dc.relation.journalcodeJ01038-
dc.identifier.eissn1879-0542-
dc.identifier.pmid28673654-
dc.identifier.urlhttps://www.sciencedirect.com/science/article/pii/S0165247817300834-
dc.subject.keywordAntigen-presenting cells-
dc.subject.keywordDendritic cells-
dc.subject.keywordGM-CSF-
dc.subject.keywordHematopoiesis-
dc.subject.keywordSpleen-
dc.contributor.alternativeNameNa, Hye Young-
dc.contributor.alternativeNamePark, Chae Gyu-
dc.contributor.affiliatedAuthorNa, Hye Young-
dc.contributor.affiliatedAuthorPark, Chae Gyu-
dc.citation.volume188-
dc.citation.startPage96-
dc.citation.endPage107-
dc.identifier.bibliographicCitationIMMUNOLOGY LETTERS, Vol.188 : 96-107, 2017-
dc.identifier.rimsid41386-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.