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Competent antigen-presenting cells are generated from the long-term culture of splenocytes with granulocyte-macrophage colony-stimulating factor
DC Field | Value | Language |
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dc.contributor.author | 나혜영 | - |
dc.contributor.author | 박채규 | - |
dc.date.accessioned | 2018-07-20T07:53:53Z | - |
dc.date.available | 2018-07-20T07:53:53Z | - |
dc.date.issued | 2017 | - |
dc.identifier.issn | 0165-2478 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/160642 | - |
dc.description.abstract | Dendritic cells (DCs) are routinely produced from the culture of mouse bone marrow (BM) with granulocyte-macrophage colony-stimulating factor (GM-CSF) within a period of 10days. Although splenic extramedullary myelopoiesis was suggested to occur under the influence of GM-CSF, the hematopoietic outcome of splenic culture with GM-CSF has not been scrutinized. We have cultured mouse splenocytes with GM-CSF for an extended period of time, where we discovered that the CD11b⁺CD11c⁺ cells began to proliferate prominently after 10days and their number increased until the 4th week of the culture. In parallel experiments, FMS-like tyrosine kinase 3 (FLT3) and its ligand, FLT3L, were not found to influence the culture of splenocytes. Like DCs in the culture of BM with GM-CSF, a distinct population of CD11b⁺CD11c⁺MHC IIhi cells was readily identified as DCs in the long-term culture of splenocytes. After being isolated and plated overnight the CD11b⁺CD11c⁺MHC IIhi cells exhibited non-adherent dendritic morphology, while the other CD11b⁺CD11c⁺ cells became adherent. Besides, these CD11b⁺CD11c⁺MHC IIhi cells possessed relatively weak endocytic and phagocytic abilities but displayed strong antigen-presenting capacities, revealing DC-like characteristics; in contrast, the other CD11b⁺CD11c⁺ cells showed strong endocytosis and phagocytosis of antigens but were poor at antigen presentation, indicating macrophage-like traits. Therefore, we demonstrated that phenotypically as well as functionally genuine DCs are generated in the long-term culture of splenocytes with GM-CSF. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Elsevier/North-Holland Biomedical Press | - |
dc.relation.isPartOf | IMMUNOLOGY LETTERS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Antigen Presentation/immunology | - |
dc.subject.MESH | Antigen-Presenting Cells/cytology | - |
dc.subject.MESH | Antigen-Presenting Cells/drug effects* | - |
dc.subject.MESH | Antigen-Presenting Cells/immunology* | - |
dc.subject.MESH | Antigen-Presenting Cells/metabolism | - |
dc.subject.MESH | Biomarkers | - |
dc.subject.MESH | Bone Marrow | - |
dc.subject.MESH | Cells | - |
dc.subject.MESH | Cell Culture Techniques | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Dendritic Cells/immunology | - |
dc.subject.MESH | Dendritic Cells/metabolism | - |
dc.subject.MESH | Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology* | - |
dc.subject.MESH | Histocompatibility Antigens Class II/immunology | - |
dc.subject.MESH | Immunophenotyping | - |
dc.subject.MESH | Membrane Proteins/metabolism | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mice, Transgenic | - |
dc.subject.MESH | Signal Transduction | - |
dc.subject.MESH | Spleen/cytology* | - |
dc.subject.MESH | Spleen/immunology* | - |
dc.subject.MESH | Spleen/metabolism | - |
dc.subject.MESH | T-Lymphocyte Subsets/immunology | - |
dc.subject.MESH | T-Lymphocyte Subsets/metabolism | - |
dc.subject.MESH | fms-Like Tyrosine Kinase 3/metabolism | - |
dc.title | Competent antigen-presenting cells are generated from the long-term culture of splenocytes with granulocyte-macrophage colony-stimulating factor | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine | - |
dc.contributor.department | Dept. of Life Science | - |
dc.contributor.googleauthor | Seul Hye Ryu | - |
dc.contributor.googleauthor | Hye Young Na | - |
dc.contributor.googleauthor | Moah Sohn | - |
dc.contributor.googleauthor | Wanho Choi | - |
dc.contributor.googleauthor | Hyunju In | - |
dc.contributor.googleauthor | Hyun Soo Shin | - |
dc.contributor.googleauthor | Jae-Hoon Choi | - |
dc.contributor.googleauthor | Chae Gyu Park | - |
dc.identifier.doi | 10.1016/j.imlet.2017.06.010 | - |
dc.contributor.localId | A04556 | - |
dc.contributor.localId | A01718 | - |
dc.relation.journalcode | J01038 | - |
dc.identifier.eissn | 1879-0542 | - |
dc.identifier.pmid | 28673654 | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0165247817300834 | - |
dc.subject.keyword | Antigen-presenting cells | - |
dc.subject.keyword | Dendritic cells | - |
dc.subject.keyword | GM-CSF | - |
dc.subject.keyword | Hematopoiesis | - |
dc.subject.keyword | Spleen | - |
dc.contributor.alternativeName | Na, Hye Young | - |
dc.contributor.alternativeName | Park, Chae Gyu | - |
dc.contributor.affiliatedAuthor | Na, Hye Young | - |
dc.contributor.affiliatedAuthor | Park, Chae Gyu | - |
dc.citation.volume | 188 | - |
dc.citation.startPage | 96 | - |
dc.citation.endPage | 107 | - |
dc.identifier.bibliographicCitation | IMMUNOLOGY LETTERS, Vol.188 : 96-107, 2017 | - |
dc.identifier.rimsid | 41386 | - |
dc.type.rims | ART | - |
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