Cited 19 times in
Renoprotective Effects of Carbon Monoxide-Releasing Molecule 3 in Ischemia-Reperfusion Injury and Cisplatin-Induced Toxicity.
DC Field | Value | Language |
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dc.contributor.author | 윤영은 | - |
dc.contributor.author | 이광석 | - |
dc.contributor.author | 이형호 | - |
dc.contributor.author | 한웅규 | - |
dc.date.accessioned | 2018-07-20T07:37:14Z | - |
dc.date.available | 2018-07-20T07:37:14Z | - |
dc.date.issued | 2017 | - |
dc.identifier.issn | 0041-1345 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/160342 | - |
dc.description.abstract | BACKGROUND: We investigated the effects of a soluble carbon monoxide-releasing molecule (CORM) in cisplatin-induced cytotoxicity and ischemia-reperfusion injury (IRI) in vitro. METHODS: The effects of CORM-3 (12.5-200 μM) were assessed in normal kidney epithelial cells (HK-2, LLC-PK1) and renal cancer cells (Caki-1, Caki-2) subjected to cisplatin (50-200 μM) or IRI. To induce IRI, cells were placed in an anaerobic chamber (37°C, 95% nitrogen, 5% carbon dioxide) for 48 hours. Cells were transferred to complete medium and incubated at 37°C, 5% carbon dioxide for 6 hours. Cell viability (CCK assays), tumor necrosis factor (TNF)-α messenger RNA (mRNA) levels (quantitative reverse-transcriptase polymerase chain reaction), and protein expression of cleaved-caspase 3 and oxidative stress markers (including Erk1/2, JNK, and P38; Western blot) were assessed. RESULTS: Viability after IRI was approximately 40% of control. Protective effects of CORM-3 in the IRI model were dose-dependent. Cell viability was 40% recovered in 200-μM CORM-3-pretreated cells compared with control. The protective effects of CORM-3 in cells exposed to cisplatin for 24 hours were weaker than in the IRI model. TNF-α mRNA was induced by stimulated IRI or cisplatin exposure; CORM-3 pretreatment attenuated the rise in TNF-α mRNA. IRI or cisplatin-induced activated oxidative stress markers decreased in CORM-3-pretreated cells. CORM-3 reduced expression of the apoptotic marker cleaved-caspase 3. CONCLUSION: Our data demonstrate the protective effects of CORM-3 in cisplatin cytotoxicity and IRI in both normal kidney cells and renal cancer cells in vitro. CORM-3 exerts these effects by ameliorating inflammatory and oxidative stress pathways. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Elsevier Science Inc. | - |
dc.relation.isPartOf | TRANSPLANTATION PROCEEDINGS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Cell Line | - |
dc.subject.MESH | Cell Survival/drug effects | - |
dc.subject.MESH | Cisplatin/toxicity* | - |
dc.subject.MESH | Epithelial Cells/drug effects* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Kidney/drug effects* | - |
dc.subject.MESH | Organometallic Compounds/pharmacology* | - |
dc.subject.MESH | Reperfusion Injury/metabolism* | - |
dc.subject.MESH | Tumor Necrosis Factor-alpha/metabolism | - |
dc.title | Renoprotective Effects of Carbon Monoxide-Releasing Molecule 3 in Ischemia-Reperfusion Injury and Cisplatin-Induced Toxicity. | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine | - |
dc.contributor.department | Dept. of Urology | - |
dc.contributor.googleauthor | Y.E. Yoon | - |
dc.contributor.googleauthor | K.S. Lee | - |
dc.contributor.googleauthor | Y.J. Lee | - |
dc.contributor.googleauthor | H.H. Lee | - |
dc.contributor.googleauthor | W.K. Han | - |
dc.identifier.doi | 10.1016/j.transproceed.2017.03.067 | - |
dc.contributor.localId | A02581 | - |
dc.contributor.localId | A02668 | - |
dc.contributor.localId | A04647 | - |
dc.contributor.localId | A04308 | - |
dc.relation.journalcode | J02755 | - |
dc.identifier.eissn | 1873-2623 | - |
dc.identifier.pmid | 28583551 | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0041134517302877 | - |
dc.contributor.alternativeName | Yoon, Young Eun | - |
dc.contributor.alternativeName | Lee, Kwang Suk | - |
dc.contributor.alternativeName | Lee, Hyung Ho | - |
dc.contributor.alternativeName | Han, Woong Kyu | - |
dc.contributor.affiliatedAuthor | Yoon, Young Eun | - |
dc.contributor.affiliatedAuthor | Lee, Kwang Suk | - |
dc.contributor.affiliatedAuthor | Lee, Hyung Ho | - |
dc.contributor.affiliatedAuthor | Han, Woong Kyu | - |
dc.citation.volume | 49 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 1175 | - |
dc.citation.endPage | 1182 | - |
dc.identifier.bibliographicCitation | TRANSPLANTATION PROCEEDINGS, Vol.49(5) : 1175-1182, 2017 | - |
dc.identifier.rimsid | 41518 | - |
dc.type.rims | ART | - |
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