0 313

Cited 8 times in

Renoprotective Effects of Carbon Monoxide-Releasing Molecule 3 in Ischemia-Reperfusion Injury and Cisplatin-Induced Toxicity.

DC Field Value Language
dc.contributor.author윤영은-
dc.contributor.author이광석-
dc.contributor.author이형호-
dc.contributor.author한웅규-
dc.date.accessioned2018-07-20T07:37:14Z-
dc.date.available2018-07-20T07:37:14Z-
dc.date.issued2017-
dc.identifier.issn0041-1345-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/160342-
dc.description.abstractBACKGROUND: We investigated the effects of a soluble carbon monoxide-releasing molecule (CORM) in cisplatin-induced cytotoxicity and ischemia-reperfusion injury (IRI) in vitro. METHODS: The effects of CORM-3 (12.5-200 μM) were assessed in normal kidney epithelial cells (HK-2, LLC-PK1) and renal cancer cells (Caki-1, Caki-2) subjected to cisplatin (50-200 μM) or IRI. To induce IRI, cells were placed in an anaerobic chamber (37°C, 95% nitrogen, 5% carbon dioxide) for 48 hours. Cells were transferred to complete medium and incubated at 37°C, 5% carbon dioxide for 6 hours. Cell viability (CCK assays), tumor necrosis factor (TNF)-α messenger RNA (mRNA) levels (quantitative reverse-transcriptase polymerase chain reaction), and protein expression of cleaved-caspase 3 and oxidative stress markers (including Erk1/2, JNK, and P38; Western blot) were assessed. RESULTS: Viability after IRI was approximately 40% of control. Protective effects of CORM-3 in the IRI model were dose-dependent. Cell viability was 40% recovered in 200-μM CORM-3-pretreated cells compared with control. The protective effects of CORM-3 in cells exposed to cisplatin for 24 hours were weaker than in the IRI model. TNF-α mRNA was induced by stimulated IRI or cisplatin exposure; CORM-3 pretreatment attenuated the rise in TNF-α mRNA. IRI or cisplatin-induced activated oxidative stress markers decreased in CORM-3-pretreated cells. CORM-3 reduced expression of the apoptotic marker cleaved-caspase 3. CONCLUSION: Our data demonstrate the protective effects of CORM-3 in cisplatin cytotoxicity and IRI in both normal kidney cells and renal cancer cells in vitro. CORM-3 exerts these effects by ameliorating inflammatory and oxidative stress pathways.-
dc.description.statementOfResponsibilityrestriction-
dc.languageEnglish-
dc.publisherElsevier Science Inc.-
dc.relation.isPartOfTRANSPLANTATION PROCEEDINGS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHCell Line-
dc.subject.MESHCell Survival/drug effects-
dc.subject.MESHCisplatin/toxicity*-
dc.subject.MESHEpithelial Cells/drug effects*-
dc.subject.MESHHumans-
dc.subject.MESHKidney/drug effects*-
dc.subject.MESHOrganometallic Compounds/pharmacology*-
dc.subject.MESHReperfusion Injury/metabolism*-
dc.subject.MESHTumor Necrosis Factor-alpha/metabolism-
dc.titleRenoprotective Effects of Carbon Monoxide-Releasing Molecule 3 in Ischemia-Reperfusion Injury and Cisplatin-Induced Toxicity.-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine-
dc.contributor.departmentDept. of Urology-
dc.contributor.googleauthorY.E. Yoon-
dc.contributor.googleauthorK.S. Lee-
dc.contributor.googleauthorY.J. Lee-
dc.contributor.googleauthorH.H. Lee-
dc.contributor.googleauthorW.K. Han-
dc.identifier.doi10.1016/j.transproceed.2017.03.067-
dc.contributor.localIdA02581-
dc.contributor.localIdA02668-
dc.contributor.localIdA04647-
dc.contributor.localIdA04308-
dc.relation.journalcodeJ02755-
dc.identifier.eissn1873-2623-
dc.identifier.pmid28583551-
dc.identifier.urlhttps://www.sciencedirect.com/science/article/pii/S0041134517302877-
dc.contributor.alternativeNameYoon, Young Eun-
dc.contributor.alternativeNameLee, Kwang Suk-
dc.contributor.alternativeNameLee, Hyung Ho-
dc.contributor.alternativeNameHan, Woong Kyu-
dc.contributor.affiliatedAuthorYoon, Young Eun-
dc.contributor.affiliatedAuthorLee, Kwang Suk-
dc.contributor.affiliatedAuthorLee, Hyung Ho-
dc.contributor.affiliatedAuthorHan, Woong Kyu-
dc.citation.volume49-
dc.citation.number5-
dc.citation.startPage1175-
dc.citation.endPage1182-
dc.identifier.bibliographicCitationTRANSPLANTATION PROCEEDINGS, Vol.49(5) : 1175-1182, 2017-
dc.identifier.rimsid41518-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Urology (비뇨의학교실) > 1. Journal Papers

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.