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Periostin-Binding DNA Aptamer Treatment Ameliorates Peritoneal Dialysis-Induced Peritoneal Fibrosis

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dc.contributor.author강신욱-
dc.contributor.author권영은-
dc.contributor.author박정탁-
dc.contributor.author유태현-
dc.contributor.author한승혁-
dc.date.accessioned2018-07-20T07:30:53Z-
dc.date.available2018-07-20T07:30:53Z-
dc.date.issued2017-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/160241-
dc.description.abstractPeritoneal fibrosis is a major complication in peritoneal dialysis (PD) patients, which leads to dialysis discontinuation. Periostin, increased by transforming growth factor β1 (TGF-β1) stimulation, induces the expression of extracellular matrix (ECM) genes. Aberrant periostin expression has been demonstrated to be associated with PD-related peritoneal fibrosis. Therefore, the effect of periostin inhibition by an aptamer-based inhibitor on peritoneal fibrosis was evaluated. In vitro, TGF-β1 treatment upregulated periostin, fibronectin, α-smooth muscle actin (α-SMA), and Snail expression and reduced E-cadherin expression in human peritoneal mesothelial cells (HPMCs). Periostin small interfering RNA (siRNA) treatment ameliorated the TGF-β1-induced periostin, fibronectin, α-SMA, and Snail expression and restored E-cadherin expression in HPMCs. Similarly, the periostin-binding DNA aptamer (PA) also attenuated fibronectin, α-SMA, and Snail upregulation and E-cadherin downregulation in TGF-β1-stimulated HPMCs. In mice treated with PD solution for 4 weeks, the expression of periostin, fibronectin, α-SMA, and Snail was significantly increased in the peritoneum, whereas E-cadherin expression was significantly decreased. The thickness of the submesothelial layer and the intensity of Masson's trichrome staining in the PD group were significantly increased compared to the untreated group. These changes were significantly abrogated by the intraperitoneal administration of PA. These findings suggest that PA can be a potential therapeutic strategy for peritoneal fibrosis in PD patients.-
dc.description.statementOfResponsibilityopen-
dc.languageUnited States-
dc.publisher2162-2531-
dc.relation.isPartOfMOLECULAR THERAPY-NUCLEIC ACIDS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titlePeriostin-Binding DNA Aptamer Treatment Ameliorates Peritoneal Dialysis-Induced Peritoneal Fibrosis-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine-
dc.contributor.departmentDept. of Internal Medicine-
dc.contributor.googleauthorBo Young Nam-
dc.contributor.googleauthorJung Tak Park-
dc.contributor.googleauthorYoung Eun Kwon-
dc.contributor.googleauthorJung Pyo Lee-
dc.contributor.googleauthorJong Ha Jung-
dc.contributor.googleauthorYoundong Kim-
dc.contributor.googleauthorSeonghun Kim-
dc.contributor.googleauthorJimin Park-
dc.contributor.googleauthorJae Eun Um-
dc.contributor.googleauthorMeiyan Wu-
dc.contributor.googleauthorSeung Hyeok Han-
dc.contributor.googleauthorTae-Hyun Yoo-
dc.contributor.googleauthorShin-Wook Kang-
dc.identifier.doi10.1016/j.omtn.2017.05.001-
dc.contributor.localIdA00053-
dc.contributor.localIdA00232-
dc.contributor.localIdA01654-
dc.contributor.localIdA02526-
dc.contributor.localIdA04304-
dc.relation.journalcodeJ03365-
dc.identifier.eissn2162-2531-
dc.identifier.pmid28624215-
dc.subject.keywordTGF-β1-
dc.subject.keywordaptamer-
dc.subject.keywordfibrosis-
dc.subject.keywordperiostin-
dc.subject.keywordperitoneal dialysis-
dc.contributor.alternativeNameKang, Shin Wook-
dc.contributor.alternativeNameKwon, Young Eun-
dc.contributor.alternativeNamePark, Jung Tak-
dc.contributor.alternativeNameYoo, Tae Hyun-
dc.contributor.alternativeNameHan, Seung Hyeok-
dc.contributor.affiliatedAuthorKang, Shin Wook-
dc.contributor.affiliatedAuthorKwon, Young Eun-
dc.contributor.affiliatedAuthorPark, Jung Tak-
dc.contributor.affiliatedAuthorYoo, Tae Hyun-
dc.contributor.affiliatedAuthorHan, Seung Hyeok-
dc.citation.volume7-
dc.citation.startPage396-
dc.citation.endPage407-
dc.identifier.bibliographicCitationMOLECULAR THERAPY-NUCLEIC ACIDS, Vol.7 : 396-407, 2017-
dc.identifier.rimsid38251-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers

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