Cited 0 times in
Comparison of COBAS AmpliPrep/COBAS TaqMan HCV Qualitative Test v2.0 with COBAS AMPLICOR Hepatitis C Virus Test v2.0 for the Qualitative Detection of Hepatitis C Virus RNA in Korean Clinical Samples
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김영란 | - |
dc.contributor.author | 김현숙 | - |
dc.contributor.author | 심정은 | - |
dc.date.accessioned | 2018-05-10T06:44:22Z | - |
dc.date.available | 2018-05-10T06:44:22Z | - |
dc.date.issued | 2013 | - |
dc.identifier.issn | 2384-2458 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/158545 | - |
dc.description.abstract | BACKGROUND: We comparatively evaluated the performance of the conventional COBAS Amplicor HCV test v2.0 (CAM; Roche Molecular Systems, USA) and the newly developed COBAS AmpliPrep/COBAS TaqMan HCV test v2.0 (CAP/CTM; Roche Molecular Systems) for qualitative detection of hepatitis C virus (HCV) RNA in clinical samples. METHODS: Six hundred serum samples (100 HCV-positive, 500 HCV-negative, as determined by CAM) were selected and analysed using the new qualitative HCV RNA test, CAP/CTM qualitative test. Results were compared by confirmatory CAP/CTM quantitative test, which is a quantitative HCV RNA real-time polymerase chain reaction by Roche Molecular Systems, and anti-HCV test (Roche Diagnostics GmbH, Germany). Twenty-two additional serum samples, which gave a gray zone result by CAM, were selected for comparison. RESULTS: The two qualitative HCV RNA assays yielded concordant results for 586 of 600 tested samples (concordance rate, 97.7%; kappa coefficient, 0.92; 95% confidence interval [CI], 0.87 to 0.96; P<0.001). Upon re-testing by CAM, we found that the concordance rate increased to 98.2% (kappa coefficient, 0.93; 95% CI, 0.89 to 0.97; P<0.001). The additional 22 samples showing gray zone results for CAM were retested and were also tested by CAP/CTM. The results for 13 of these samples changed to negative and were now concordant with the CAP/CTM and confirmatory CAP/CTM quantitative results. For the remaining samples, the results were variable. For all the 22 samples, the results of the new CAP/CTM were in agreement with those obtained by confirmatory CAP/CTM quantitative test. CONCLUSIONS: The results of the two assays were in good agreement, with 97.7% concordance rate. However, CAP/CTM is more sensitive than CAM and showed no gray zone results. Therefore, it can be a more efficient and useful test for the qualitative detection of HCV RNA in clinical samples. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | Korean | - |
dc.publisher | 대한임상검사정도관리협회 | - |
dc.relation.isPartOf | Journal of Laboratory Medicine and Quality Assurance (임상검사와 정도관리) | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Comparison of COBAS AmpliPrep/COBAS TaqMan HCV Qualitative Test v2.0 with COBAS AMPLICOR Hepatitis C Virus Test v2.0 for the Qualitative Detection of Hepatitis C Virus RNA in Korean Clinical Samples | - |
dc.type | Article | - |
dc.contributor.college | Others | - |
dc.contributor.department | Severance Hospital | - |
dc.contributor.googleauthor | Soohun Yoo | - |
dc.contributor.googleauthor | Younhee Park | - |
dc.contributor.googleauthor | Hwan Sub Lim | - |
dc.contributor.googleauthor | Young Ran Kim | - |
dc.contributor.googleauthor | Jung Eun Sim | - |
dc.contributor.googleauthor | Hyon Suk Kim | - |
dc.contributor.localId | A04904 | - |
dc.contributor.localId | A01117 | - |
dc.contributor.localId | A04971 | - |
dc.relation.journalcode | J01555 | - |
dc.subject.keyword | Hepatitis C virus | - |
dc.subject.keyword | Qualitative HCV RNA assay | - |
dc.subject.keyword | COBAS Amplicor | - |
dc.subject.keyword | COBAS AmpliPrep/Cobas TaqMan | - |
dc.subject.keyword | Quantitative HCV RNA real-time polymerase chain reaction (PCR) | - |
dc.contributor.alternativeName | Kim, Young Lan | - |
dc.contributor.alternativeName | Kim, Hyon Suk | - |
dc.contributor.alternativeName | Shim, Jung Eun | - |
dc.contributor.affiliatedAuthor | Kim, Young Lan | - |
dc.contributor.affiliatedAuthor | Kim, Hyon Suk | - |
dc.contributor.affiliatedAuthor | Shim, Jung Eun | - |
dc.citation.volume | 35 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 93 | - |
dc.citation.endPage | 99 | - |
dc.identifier.bibliographicCitation | Journal of Laboratory Medicine and Quality Assurance (임상검사와 정도관리), Vol.35(2) : 93-99, 2013 | - |
dc.identifier.rimsid | 43135 | - |
dc.type.rims | ART | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.