Cited 34 times in
Regulation of wound healing by granulocyte-macrophage colony-stimulating factor after vocal fold injury
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 임재열 | - |
dc.date.accessioned | 2018-05-10T06:44:17Z | - |
dc.date.available | 2018-05-10T06:44:17Z | - |
dc.date.issued | 2013 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/158541 | - |
dc.description.abstract | OBJECTIVES: Vocal fold (VF) scarring remains a therapeutic challenge. Granulocyte-macrophage colony-stimulating factor (GM-CSF) facilitates epithelial wound healing, and recently, growth factor therapy has been applied to promote tissue repair. This study was undertaken to investigate the effect of GM-CSF on VF wound healing in vivo and in vitro. METHODS: VF scarring was induced in New Zealand white rabbits by direct injury. Immediately thereafter, either GM-CSF or PBS was injected into the VFs of rabbits. Endoscopic, histopathological, immunohistochemical, and biomechanical evaluations of VFs were performed at 3 months post-injury. Human vocal fold fibroblasts (hVFFs) were cultured with GM-CSF. Production of type I and III collagen was examined immunocytochemically, and the synthesis of elastin and hyaluronic acids was evaluated by ELISA. The mRNA levels of genes related to ECM components and ECM production-related growth factors, such as HGF and TGF-ß1, were examined by real time RT-PCR. RESULTS: The GM-CSF-treated VFs showed reduced collagen deposition in comparison to the PBS-injected controls (P<0.05). Immunohistochemical staining revealed lower amounts of type I collagen and fibronectin in the GM-CSF-treated VFs (P<0.05 and P<0.01, respectively). Viscous and elastic shear moduli of VF samples were significantly lower in the GM-CSF group than in the PBS-injected group (P<0.001 and P<0.01, respectively). Mucosal waves in the GM-CSF group showed significant improvement when compared to the PBS group (P = 0.0446). GM-CSF inhibited TGF-β1-induced collagen synthesis by hVFFs (P<0.05) and the production of hyaluronic acids increased at 72 hours post-treatment (P<0.05). The expressions of HAS-2, tropoelastin, MMP-1, HGF, and c-Met mRNA were significantly increased by GM-CSF, although at different time points (P<0.05). CONCLUSION: The present study shows that GM-CSF offers therapeutic potential for the remodeling of VF wounds and the promotion of VF regeneration. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Public Library of Science | - |
dc.relation.isPartOf | PLOS ONE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Collagen Type I/antagonists & inhibitors | - |
dc.subject.MESH | Collagen Type I/genetics | - |
dc.subject.MESH | Collagen Type I/metabolism | - |
dc.subject.MESH | Collagen Type III/genetics | - |
dc.subject.MESH | Collagen Type III/metabolism | - |
dc.subject.MESH | Elastin/genetics | - |
dc.subject.MESH | Elastin/metabolism | - |
dc.subject.MESH | Extracellular Matrix/drug effects | - |
dc.subject.MESH | Extracellular Matrix/genetics | - |
dc.subject.MESH | Extracellular Matrix/metabolism | - |
dc.subject.MESH | Fibroblasts/cytology | - |
dc.subject.MESH | Fibroblasts/drug effects* | - |
dc.subject.MESH | Fibroblasts/metabolism | - |
dc.subject.MESH | Fibronectins/antagonists & inhibitors | - |
dc.subject.MESH | Fibronectins/genetics | - |
dc.subject.MESH | Fibronectins/metabolism | - |
dc.subject.MESH | Gene Expression/drug effects | - |
dc.subject.MESH | Granulocyte-Macrophage Colony-Stimulating Factor/metabolism | - |
dc.subject.MESH | Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology* | - |
dc.subject.MESH | Hepatocyte Growth Factor/genetics | - |
dc.subject.MESH | Hepatocyte Growth Factor/metabolism | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Hyaluronic Acid/biosynthesis | - |
dc.subject.MESH | Injections, Intralesional | - |
dc.subject.MESH | Rabbits | - |
dc.subject.MESH | Transforming Growth Factor beta1/genetics | - |
dc.subject.MESH | Transforming Growth Factor beta1/metabolism | - |
dc.subject.MESH | Vocal Cords/drug effects* | - |
dc.subject.MESH | Vocal Cords/injuries* | - |
dc.subject.MESH | Vocal Cords/metabolism | - |
dc.subject.MESH | Wound Healing/drug effects* | - |
dc.subject.MESH | Wound Healing/physiology | - |
dc.title | Regulation of wound healing by granulocyte-macrophage colony-stimulating factor after vocal fold injury | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine | - |
dc.contributor.department | Dept. of Otorhinolaryngology | - |
dc.contributor.googleauthor | Jae-Yol Lim | - |
dc.contributor.googleauthor | Byung Hyune Choi | - |
dc.contributor.googleauthor | Songyi Lee | - |
dc.contributor.googleauthor | Yun Ho Jang | - |
dc.contributor.googleauthor | Jeong-Seok Choi | - |
dc.contributor.googleauthor | Young-Mo Kim | - |
dc.identifier.doi | 10.1371/journal.pone.0054256 | - |
dc.contributor.localId | A03396 | - |
dc.relation.journalcode | J02540 | - |
dc.identifier.eissn | 1932-6203 | - |
dc.identifier.pmid | 23372696 | - |
dc.contributor.alternativeName | Lim, Jae Yol | - |
dc.contributor.affiliatedAuthor | Lim, Jae Yol | - |
dc.citation.volume | 8 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | e54256 | - |
dc.identifier.bibliographicCitation | PLOS ONE, Vol.8(1) : e54256, 2013 | - |
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