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Autonomous control of terminal erythropoiesis via physical interactions among erythroid cells

DC Field Value Language
dc.contributor.author김현옥-
dc.date.accessioned2018-05-10T06:42:29Z-
dc.date.available2018-05-10T06:42:29Z-
dc.date.issued2013-
dc.identifier.issn1873-5061-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/158473-
dc.description.abstractIn vitro erythropoiesis has been studied extensively for its application in the manufacture of transfusable erythrocytes. Unfortunately, culture conditions have not been as effective as in vivo growth conditions, where bone marrow macrophages are known to be a key regulator of erythropoiesis. This study focused on the fact that some erythroblasts are detached from macrophages and only contact other erythroblasts. We hypothesized that additional factors regulate erythroblasts, likely through either physical contact or secreted factors. To further elucidate these critical factors, human erythroblasts derived from cord blood were cultured at high density to mimic marrow conditions. This growth condition resulted in a significantly increased erythroid enucleation rate and viability. We found several novel contact-related signals in erythroblasts: intercellular adhesion molecule-4 (ICAM-4) and deleted in liver cancer-1 (DLC-1). DLC-1, a Rho-GTPase-activating protein, has not previously been reported in erythroid cells, but its interaction with ICAM-4 was demonstrated here. We further confirmed the presence of a secreted form of human ICAM-4 for the first time. When soluble ICAM-4 was added to media, cell viability and enucleation increased with decreased nuclear dysplasia, suggesting that ICAM-4 is a key factor in contact between cells. These results highlight potential new mechanisms for autonomous control of erythropoiesis. The application of these procedures to erythrocyte manufacturing could enhance in vitro erythrocyte production for clinical use.-
dc.description.statementOfResponsibilityopen-
dc.languageEnglish-
dc.publisherElsevier-
dc.relation.isPartOfSTEM CELL RESEARCH-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHCell Adhesion Molecules/genetics-
dc.subject.MESHCell Adhesion Molecules/metabolism-
dc.subject.MESHCell Adhesion Molecules/pharmacology-
dc.subject.MESHCell Communication-
dc.subject.MESHCell Lineage-
dc.subject.MESHCells, Cultured-
dc.subject.MESHCulture Media, Conditioned/pharmacology-
dc.subject.MESHCytokinesis-
dc.subject.MESHErythroblasts/cytology*-
dc.subject.MESHErythroblasts/metabolism-
dc.subject.MESHErythropoiesis/drug effects-
dc.subject.MESHFetal Blood/cytology-
dc.subject.MESHGTPase-Activating Proteins/metabolism-
dc.subject.MESHHumans-
dc.subject.MESHK562 Cells-
dc.subject.MESHMacrophages/cytology-
dc.subject.MESHMacrophages/metabolism-
dc.subject.MESHProtein Binding-
dc.subject.MESHRecombinant Proteins/biosynthesis-
dc.subject.MESHRecombinant Proteins/genetics-
dc.subject.MESHRecombinant Proteins/pharmacology-
dc.subject.MESHTumor Suppressor Proteins/metabolism-
dc.titleAutonomous control of terminal erythropoiesis via physical interactions among erythroid cells-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine-
dc.contributor.departmentDept. of Laboratory Medicine-
dc.contributor.googleauthorHye Sook Choi-
dc.contributor.googleauthorEun Mi Lee-
dc.contributor.googleauthorHyunOkKim-
dc.contributor.googleauthorMoon-Il Park-
dc.contributor.googleauthorEun Jung Ba다-
dc.identifier.doi10.1016/j.scr.2013.02.003-
dc.contributor.localIdA01122-
dc.relation.journalcodeJ02680-
dc.identifier.eissn1876-7753-
dc.identifier.pmid23500644-
dc.contributor.alternativeNameKim, Hyun Ok-
dc.contributor.affiliatedAuthorKim, Hyun Ok-
dc.citation.volume10-
dc.citation.number3-
dc.citation.startPage442-
dc.citation.endPage453-
dc.identifier.bibliographicCitationSTEM CELL RESEARCH, Vol.10(3) : 442-453, 2013-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers

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