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Enhanced human endothelial progenitor cell adhesion and differentiation by a bioinspired multifunctional nanomatrix

 Adinarayana Andukuri  ;  Young-Doug Sohn  ;  Chidinma P. Anakwenze  ;  Dong-Jin Lim  ;  Brigitta C. Brott  ;  Young-Sup Yoon  ;  Ho-Wook Jun 
 TISSUE ENGINEERING PART C-METHODS, Vol.19(5) : 375-385, 2013 
Journal Title
Issue Date
Amino Acid Sequence ; Biocompatible Materials/pharmacology* ; Biomarkers/metabolism ; Cell Adhesion/drug effects ; Cell Differentiation/drug effects* ; Endothelial Cells/cytology* ; Endothelial Cells/drug effects ; Endothelial Cells/metabolism ; Flow Cytometry Humans ; Ligands ; Molecular Sequence Data ; Nanoparticles/chemistry* ; Nitric Oxide/metabolism ; Peptides/chemistry ; Staining and Labeling ; Stem Cells/cytology* ; Stem Cells/drug effects ; Stem Cells/metabolism ; Tissue Scaffolds/chemistry*
Endothelial progenitor cell (EPC)-capturing techniques have led to revolutionary strategies that can improve the performance of cardiovascular implant devices and engineered tissues by enhancing re-endothelialization and angiogenesis. However, these strategies are limited by controversies regarding the phenotypic identities of EPCs as well as their inability to target and prevent the other afflictions associated with current therapies, namely, thrombosis and neointimal hyperplasia. Therefore, the goal of this study was to study the efficacy of a bioinspired multifunctional nanomatrix in recruiting and promoting the differentiation of EPCs toward an endothelial lineage. The bioinspired nanomatrix combines multiple components, including self-assembled peptide amphiphiles (PAs) that include cell adhesive ligands, nitric oxide (NO)-producing donors, and enzyme-mediated degradable sequences to achieve an endothelium-mimicking character. In this study, human peripheral blood mononuclear cells (PBMNCs) were isolated and cultured on the bioinspired multifunctional nanomatrix. Initial cell adhesion, lectin staining, acetylated low-density lipoprotein uptake, and expression of endothelial markers, including CD31, CD34, von Willebrand Factor, and VEGFR2, were analyzed. The results from this study indicate that the NO releasing bioinspired multifunctional nanomatrix promotes initial adhesion of EPCs when compared to control surfaces. The expression of endothelial markers is also increased on the bioinspired multifunctional nanomatrix, suggesting that it directs the differentiation of EPCs toward an endothelial phenotype. The bioinspired nanomatrix therefore provides a novel biomaterial-based platform for capturing as well as directing EPC behavior. Therefore, this study has the potential to positively impact the patency of cardiovascular devices such as stents and vascular grafts as well as enhanced angiogenesis for ischemic or engineered tissues.
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1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
Yonsei Authors
Yoon, Young Sup(윤영섭)
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